FlyBase Allele Report: Dmel\mir-8[Δ]

General Information

Symbol

Dmel\mir-8^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0297315

Feature type

allele

Associated gene

Dmel\mir-8

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lu et al., 2014)

Mutagen

FLPase

(Lu et al., 2014)

Progenitor genotype

P{XP}d01682

(Lu et al., 2014)

PBac{WH}f05125

(Lu et al., 2014)

Cytology

Description

FLP-mediated recombination between the two progenitor insertions P{XP}d01682 and PBac{WH}f05125 has deleted the sequence between them, removing the mir-8 miRNA.

(Lu et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage | semidominant

(Lu et al., 2014)

abnormal neuroanatomy | second instar larval stage

(Loya et al., 2014)

abnormal neuroanatomy | third instar larval stage

(Loya et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous larvae show a decrease in the number of type 1b synaptic boutons and the number of axonal branches at the neuromuscular junction compared to controls at the second and third instar stages. There is a significant reduction in subsynaptic reticulum thickness, area and fold complexity compared to controls, while presynaptic bouton area is normal.

(Loya et al., 2014)

In both wild type and mir-8^Δ/mir-8^Δ embryos, the intersegmental nerve branch ISNb motor axons show normal trajectories to reach ventral muscle targets; subsequent to target recognition, mir-8^Δ/mir-8^Δ embryos show an innervation defect (innervation of the cleft between m6 and m7 is undetectable or reduced). mir-8^Δ/+ embryos show an ISNb defect at an intermediate frequency. mir-8^Δ/mir-8^Δ embryos show significant decreases in synaptic contact at m6/m7, compared to controls. RP3 axon terminals in 15h old mutant embryos show abnormalities: around 50% of RP3 motor axons show sprouting of filopodia significantly increases (with less well-defined elaboration of the neuromuscular junction between m6/m7), and RP3 axon terminals frequently extend to and form varicosities on neighboring non-target muscle (m13).

(Lu et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-8^Δ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Lmon\actA^{FP4mito.UAS.EGFP}/Scer\GAL4^how-24B

(Loya et al., 2014)

mir-8^Δ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^elav.PU

(Lu et al., 2014)

mir-8^Δ has abnormal neuroanatomy | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^how-24B

(Lu et al., 2014)

NOT suppressed by

Statement

Reference

mir-8^Δ has abnormal neuroanatomy | semidominant | embryonic stage phenotype, non-suppressible by ena²³/ena[+]

(Lu et al., 2014)

mir-8^Δ has abnormal neuroanatomy | semidominant | embryonic stage phenotype, non-suppressible by ena[+]/ena²¹⁰

(Lu et al., 2014)

Other

Statement

Reference

cpb[+]/cpb^F44, mir-8^Δ has abnormal neuroanatomy | dominant | third instar larval stage phenotype

(Loya et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-8^Δ has subsynaptic reticulum | third instar larval stage phenotype, suppressible by Lmon\actA^{FP4mito.UAS.EGFP}/Scer\GAL4^how-24B

(Loya et al., 2014)

mir-8^Δ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^elav.PU

(Lu et al., 2014)

mir-8^Δ has axon | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^elav.PU

(Lu et al., 2014)

mir-8^Δ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^how-24B

(Lu et al., 2014)

mir-8^Δ has axon | embryonic stage phenotype, suppressible | partially by Nrg^180.I.UAS/Scer\GAL4^how-24B

(Lu et al., 2014)

NOT suppressed by

Statement

Reference

mir-8^Δ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by ena²³/ena[+]

(Lu et al., 2014)

mir-8^Δ has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, non-suppressible by ena[+]/ena²¹⁰

(Lu et al., 2014)

mir-8^Δ has axon | embryonic stage phenotype, non-suppressible by ena²³/ena[+]

(Lu et al., 2014)

mir-8^Δ has axon | embryonic stage phenotype, non-suppressible by ena[+]/ena²¹⁰

(Lu et al., 2014)

Other

Statement

Reference

cpb[+]/cpb^F44, mir-8^Δ has NMJ bouton | third instar larval stage phenotype

(Loya et al., 2014)

cpb[+]/cpb^F44, mir-8^Δ has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Loya et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

mir-8^Δ/+ ; cpb^F44/+ double transheterozygous larvae show a decrease in the number of boutons and in presynaptic arbor expansion at the neuromuscular junction (normalised to muscle area) compared to controls.

(Loya et al., 2014)

ena²³/+ or ena²¹⁰/+ does not rescue the neuromuscular junction formation phenotype (intersegmental nerve branch ISNb motor axon innervation defects at m6/7) seen in mir-8^Δ/+ embryos.

Expression of Nrg^{180.I.Scer\UAS} driven by Scer\GAL4^elav.PU significantly partially suppresses the intersegmental nerve branch ISNb motor axon innervation defects (at m6/7) seen in mir-8^Δ/mir-8^Δ embryos. Expression of Nrg^{180.I.Scer\UAS} driven by Scer\GAL4^how-24B significantly partially (only 10%) suppresses the intersegmental nerve branch ISNb motor axon innervation defects (of m6/7) seen in mir-8^Δ/mir-8^Δ embryos.

(Lu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^how-24B rescues the reduction in subsynaptic reticulum area seen at the neuromuscular junction in homozygous mir-8^Δ larvae.

(Loya et al., 2014)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

miR-8^Δ

(Loya et al., 2014, Lu et al., 2014)

mir-8^Δ

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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