FlyBase Allele Report: Dmel\nkd[3]

General Information

Symbol

Dmel\nkd³

Species

D. melanogaster

Name

FlyBase ID

FBal0013025

Feature type

allele

Associated gene

Dmel\nkd

Associated Insertion(s)

Carried in Construct

Also Known As

nkd^7H16

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q60term.

(Zeng et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,010,945..19,010,945 [-]

Nucleotide change:

C19010945T

Amino acid change:

Q60term | nkd-PA; Q60term | nkd-PB; Q60term | nkd-PC

Reported amino acid change:

Q60term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Zeng et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Zeng et al., 2000)

some die during embryonic stage | recessive

(Zeng et al., 2000)

visible | somatic clone

(Rousset et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant embryos secrete a uniform naked cuticle.

(Chan et al., 2007)

nkd²/nkd³ embryos have a strong cuticle phenotype with a severe head involution defect and widely split posterior spiracles.

(Waldrop et al., 2006)

nkd²/nkd³ embryos have a strong cuticle phenotype; the cuticles are less than 75% of wild-type length, have two or less complete denticle bands and have an exteriorized head skeleton and noneverted spiracles.

(Rousset et al., 2002)

Homozygous mutant nkd³ eyes, generated by somatic clone, are small and rough. Homozygous embryos lack denticle belts.

(Rousset et al., 2001)

Homozygous embryos have a strong cuticle phenotype; they secrete cuticle with a fully exteriorised head skeleton, widely split filzkorper and residual denticle belts only in segment A3 and/or A5 (or no denticle belts at all), and are typically less than 75% of wild-type length.

(Zeng et al., 2000)

ptc parasegmental boundaries are shifted in nkd embryos forming posterior to each en domain: expression domain is reduced.

(Hidalgo and Ingham, 1990)

nkd mutant embryos have Keilin's organs, but these often have four or more sensory hairs rather than the normal three. Occasionally extra, discrete organs are seen, so that three organs appear in one segment, usually accompanied by the loss of an organ from an adjacent segment. nkd³/nkd² embryos grew in in vivo culture, producing implants containing imaginal discs and all larval tissues. The discs tended to grow as a merged sheet of material. Metamorphosed implants produced adult cuticular structures derived from the eye-antenna discs, leg and wing discs.

(Simcox et al., 1989)

strong allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

hry¹, nkd³ has visible | somatic clone phenotype

(Zeng et al., 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

nkd³ has embryonic/first instar larval cuticle phenotype, suppressible by Scer\GAL4^ptc-559.1/pan^ΔN.UAS

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, suppressible by Scer\GAL4^ptc-559.1/pan^ΔN.UAS

(Chan et al., 2007)

nkd³ has embryonic/first instar larval cuticle phenotype, suppressible by Scer\GAL4^en-e16E/pan^ΔN.UAS

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, suppressible by Scer\GAL4^en-e16E/pan^ΔN.UAS

(Chan et al., 2007)

nkd³ has embryonic/first instar larval cuticle phenotype, suppressible by nkd::Mmus\Nkd1^{fNf30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, suppressible by nkd::Mmus\Nkd1^{fNf30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³/nkd² has embryonic/first instar larval cuticle phenotype, suppressible | partially by nkd::Btau\recoverin^{rEF.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² has embryonic/first instar larval cuticle phenotype, suppressible | partially by nkd::Hsap\NKD2^{hEFX2.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

NOT suppressed by

Statement

Reference

nkd³ has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{m1NΔN.UAS.GFP}

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, non-suppressible by Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{m1NΔN.UAS.GFP}

(Chan et al., 2007)

nkd³ has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, non-suppressible by Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}

(Chan et al., 2007)

nkd³ has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/Mmus\Nkd1^UAS.EGFP

(Chan et al., 2007)

nkd³ has ventral denticle belt phenotype, non-suppressible by Scer\GAL4^da.G32/Mmus\Nkd1^UAS.EGFP

(Chan et al., 2007)

nkd³/nkd² has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/Mmus\Nkd1^UAS.EGFP

(Waldrop et al., 2006)

nkd³/nkd² has embryonic/first instar larval cuticle phenotype, non-suppressible by Scer\GAL4^da.G32/nkd::Mmus\Nkd1^{f30aa.UAS.EGFP}

(Waldrop et al., 2006)

NOT Enhancer of

Statement

Reference

nkd³ is a non-enhancer of phenotype of dsh³

(Rousset et al., 2001)

nkd³ is a non-enhancer of phenotype of arm⁴

(Rousset et al., 2001)

NOT Suppressor of

Statement

Reference

nkd³ is a non-suppressor of phenotype of arm⁴

(Rousset et al., 2001)

nkd³ is a non-suppressor of phenotype of dsh³

(Rousset et al., 2001)

Other

Statement

Reference

hry¹, nkd³/nkd¹³ has ventral denticle belt | embryonic stage phenotype

(Gammons et al., 2020)

hry¹, nkd³/nkd² has ventral denticle belt | embryonic stage phenotype

(Gammons et al., 2020)

hry¹, nkd³ has ventral denticle belt | embryonic stage | absent phenotype

(Gammons et al., 2020)

hry¹, nkd³ has ommatidium | somatic clone phenotype

(Zeng et al., 2000)

hry¹, nkd³ has eye | somatic clone phenotype

(Zeng et al., 2000)

hry¹, nkd³ has wing margin bristle | somatic clone phenotype

(Zeng et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

h¹ nkd³ double homozygous, h¹/h¹ nkd³/nkd¹³ double mutant or h¹/h¹ nkd²/nkd³ double mutant embryos exhibit defective denticle belts.

(Gammons et al., 2020)

The naked cuticle phenotype of nkd³ embryos is partially rescued by expression of pan^ΔN.Scer\UAS under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^en-e16E, with more complete rescue being seen when Scer\GAL4^ptc-559.1 is used as the driver.

(Chan et al., 2007)

nkd³ h¹ clones give rise to a rough eye phenotype and loss of wing margin bristles.

(Zeng et al., 2000)

Xenogenetic Interactions

Statement

Reference

Expression of nkd::Mmus\Nkd1^{m1NΔN.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^da.G32 results in little or no rescue of the nkd³ cuticle phenotype.

Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 does not rescue the nkd³ cuticle phenotype.

Expression of Mmus\Nkd1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 does not rescue the nkd³ cuticle phenotype.

Expression of nkd::Mmus\Nkd1^{fNf30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 partially rescues the nkd³ cuticle phenotype.

(Chan et al., 2007)

Expression of nkd::Btau\recoverin^{rEF.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^da.G32 largely rescues the cuticle defects of nkd²/nkd³ embryos and adult viability is restored.

Expression of nkd::Hsap\NKD2^{hEFX2.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^da.G32 largely rescues the cuticle defects of nkd²/nkd³ embryos and adult viability is restored.

Expression of Mmus\Nkd1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 fails to rescue the cuticle defects of nkd²/nkd³ embryos.

Expression of nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^da.G32 fails to rescue the cuticle defects of nkd²/nkd³ embryos.

(Waldrop et al., 2006)

When Xlae\Gsk3β^cRa RNA is injected into nkd³ flies a suppression of the naked cuticle phenotype is seen leading to the production of some denticle belts.

(Rousset et al., 2001)

Complementation and Rescue Data

Fails to complement

nkd^04869a

(Zeng et al., 2000)

Rescued by

nkd³ is rescued by nkd^UAS.EGFP/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is rescued by nkd^UAS.EGFP/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³/nkd² is rescued by Scer\GAL4^da.G32/nkd^UAS.cZa

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^{ΔEFX.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^NBg.UAS.EGFP/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^NGA.UAS.EGFP/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by Scer\GAL4^da.G32/nkd^{NIN4.UAS.EGFP}

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^{ΔRB.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^UAS.EGFP/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by nkd^UAS.Tag:MYC/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³ is rescued by nkd^UAS.EGFP/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is rescued by Scer\GAL4^da.G32/nkd^UAS.cZa

(Rousset et al., 2002)

nkd³/nkd² is rescued by nkd^{D201A.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Rousset et al., 2002)

nkd³ is rescued by nkd^hs.Tag:MYC

(Zeng et al., 2000)

Partially rescued by

nkd³ is partially rescued by nkd^{ΔR1S.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{Δ30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by Scer\GAL4^da.G32/nkd^{Δ30aa.UAS.Tag:NLS(SV40-largeT),EGFP}

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{ΔR1SΔ30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{ΔD6.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{ΔR1SΔD6.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{R441A.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{K445A.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by nkd^{RAKA.UAS.EGFP}/Scer\GAL4^da.G32

(Chan et al., 2008)

nkd³ is partially rescued by Scer\GAL4^prd.RG1/nkd^UAS.EGFP

(Chan et al., 2007)

nkd³ is partially rescued by Scer\GAL4^en-e16E/nkd^UAS.cZa

(Chan et al., 2007)

nkd³ is partially rescued by Scer\GAL4^ptc-559.1/nkd^UAS.cZa

(Chan et al., 2007)

nkd³ is partially rescued by nkd^{GFPX.UAS.EGFP,Tag:MYC}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ is partially rescued by nkd^NBg.UAS.EGFP/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ is partially rescued by nkd^{NBg.UAS.Tag:Myr(Src64B),EGFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³/nkd² is partially rescued by nkd^{D201A.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is partially rescued by nkd^{NIN3.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is partially rescued by nkd^{ΔYS.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is partially rescued by nkd^{ΔR1S.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is partially rescued by nkd^{Δ30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³ is partially rescued by nkd^{ΔR1S.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³ is partially rescued by nkd^{Δ30aa.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³ is partially rescued by Scer\GAL4^da.G32/nkd^{Δ30aa.UAS.Tag:NLS(SV40-largeT),EGFP}

(Waldrop et al., 2006)

nkd³/nkd² is partially rescued by nkd^{NIN3.UAS.EGFP}/Scer\GAL4^da.G32

(Rousset et al., 2002)

Not rescued by

nkd³ is not rescued by Scer\GAL4^da.G32/nkd^{ΔR1SΔ30aaNLS.UAS.Tag:NLS(SV40-largeT),EGFP}

(Chan et al., 2008)

nkd³ is not rescued by Scer\GAL4^da.G32/nkd^{ΔR1SΔD6Δ30aa.UAS.EGFP}

(Chan et al., 2008)

nkd³ is not rescued by nkd^{GFPN.UAS.EGFP,Tag:MYC}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ is not rescued by nkd^{ΔN.GFPC.UAS.GFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ is not rescued by nkd^{NBg.UAS.SrcGTA,EGFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³ is not rescued by nkd^{SrcRBg.UAS.Tag:Myr(Src64B),EGFP}/Scer\GAL4^da.G32

(Chan et al., 2007)

nkd³/nkd² is not rescued by nkd^{NBam.UAS.EGFP}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by Scer\GAL4^da.G32/nkd^{NR1.UAS.Tag:MYC}

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by Scer\GAL4^da.G32/nkd^{ΔBBg.UAS.EGFP}

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by Scer\GAL4^da.G32/nkd^{ΔRBg.UAS.EGFP}

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by nkd^7H.UAS.EGFP/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by nkd^{7H.UAS.Tag:MYC}/Scer\GAL4^da.G32

(Waldrop et al., 2006)

nkd³/nkd² is not rescued by nkd^EFX.UAS.EGFP/Scer\GAL4^da.G32

(Rousset et al., 2002)

nkd³/nkd² is not rescued by Scer\GAL4^da.G32/nkd^{R1S.UAS.Tag:MYC}

(Rousset et al., 2002)

nkd³/nkd² is not rescued by nkd^{NBam.UAS.EGFP}/Scer\GAL4^da.G32

(Rousset et al., 2002)

nkd³/nkd² is not rescued by Scer\GAL4^da.G32/nkd^{NR1.UAS.Tag:MYC}

(Rousset et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (4)

Bloomington

25112

w^*; hry¹ nkd³ P{neoFRT}80B/TM6B, Tb⁺

25383

w^*; P{UAS-nkd.myc}3-2; nkd³/TM3, P{HB-lacZ}GS2, Sb¹

26254

w^*; P{UAS-nkd.ΔN.GFPC}2; nkd³/TM3, P{HB-lacZ}GS2, Sb¹

26255

w^*; P{UAS-nkd.ΔD6.GFPC}2; nkd³/TM3, P{HB-lacZ}GS2, Sb¹

Notes on Origin

Discoverer

Comments

The expression of l(1)sc is unaltered in early homozygous mutant embryos.

(Martin-Bermudo et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nkd³

(Abdou et al., 2011)

nkd^7H16

(Gammons et al., 2020, Chan et al., 2008, Chan et al., 2007, Waldrop et al., 2006, Rousset et al., 2002, Rousset et al., 2001, Zeng et al., 2000, Jagla et al., 1997, Irvine and Wieschaus, 1994, Li and Noll, 1993, van den Heuvel et al., 1993, Hidalgo, 1991, Martin-Bermudo et al., 1991, Hidalgo and Ingham, 1990, Simcox et al., 1989)

nkd^7H

(Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (18)

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