FlyBase Allele Report: Dmel\nkd[UAS.cZa]

General Information

Symbol

Dmel\nkd^UAS.cZa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Zeng

FlyBase ID

FBal0104471

Feature type

allele

Associated gene

Dmel\nkd

Associated Insertion(s)

Carried in Construct

P{UAS-nkd.Z}

Also Known As

UAS-Nkd

Key Links

Transgenic product class

wild_type

(Zeng et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zeng et al., 2000)

Mutagen

in vitro construct

(Zeng et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-nkd.Z}

Cytology

Description

UAS regulatory sequences drive nkd expression.

(Zeng et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

nkd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^B119

(Rousset et al., 2002, Zeng et al., 2000)

visible, with Scer\GAL4^{l(2)E105-E105}

(Zeng et al., 2000)

visible | adult stage, with Scer\GAL4^en.PU

(Wang et al., 2021)

Phenotype Manifest In

abdominal sternite bristle, with Scer\GAL4^B119

(Rousset et al., 2002)

abdominal sternite bristle, with Scer\GAL4^unspecified

(Zeng et al., 2000)

antenna, with Scer\GAL4^unspecified

(Zeng et al., 2000)

anterior crossvein | ectopic, with Scer\GAL4^unspecified

(Zeng et al., 2000)

eye | ventral, with Scer\GAL4^unspecified

(Zeng et al., 2000)

haltere, with Scer\GAL4^unspecified

(Zeng et al., 2000)

leg, with Scer\GAL4^B119

(Zeng et al., 2000)

leg, with Scer\GAL4^{l(2)E105-E105}

(Zeng et al., 2000)

leg, with Scer\GAL4^unspecified

(Zeng et al., 2000)

leg | ectopic, with Scer\GAL4^B119

(Zeng et al., 2000)

leg | ectopic, with Scer\GAL4^unspecified

(Zeng et al., 2000)

neuroblast NB7-3 | ectopic, with Scer\GAL4^en-e16E

(Deshpande et al., 2001)

scutum | ectopic, with Scer\GAL4^unspecified

(Zeng et al., 2000)

wing, with Scer\GAL4^ap-md544

(Jones et al., 2010)

wing, with Scer\GAL4^unspecified

(Zeng et al., 2000)

wing margin, with Scer\GAL4^en.PU

(Wang et al., 2021)

wing margin bristle, with Scer\GAL4^unspecified

(Zeng et al., 2000)

Detailed Description

Statement

Reference

Flies overexpressing nkd^Scer\UAS.cZa driven by Scer\GAL4^ap-md544 show wings that are reduced in size and blistered.

(Jones et al., 2010)

Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^B119 results in near total loss of sternite bristles with occasional residual laterally displaced bristles.

(Rousset et al., 2002)

A duplicated NB 7-3 is formed in 40% of hemisegments in embryos expressing nkd^Scer\UAS.cZa under the control of Scer\GAL4^en-e16E.

(Deshpande et al., 2001)

Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^{l(2)E105-E105} results in variable truncation of the legs. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^B119 results in variable duplication of the legs. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^unspecified results in a number of phenotypes including wing to notum transformations, leg truncations and duplications, loss, lateral displacement and disorientation of sternite bristles, haltere loss, ventral eye reduction, loss of wing margin, extra wing anterior crossveins and loss of antennae.

(Zeng et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by porⁱ⁸

(Zeng et al., 2000)

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by wg^l-12

(Zeng et al., 2000)

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by wg^l-17

(Zeng et al., 2000)

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by dsh⁴⁷⁷

(Zeng et al., 2000)

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by dsh³

(Zeng et al., 2000)

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, enhanceable by arm⁴

(Zeng et al., 2000)

Suppressed by

Statement

Reference

Scer\GAL4^unspecified, nkd^UAS.cZa has visible phenotype, suppressible by sgg^M11

(Zeng et al., 2000)

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/nkd^UAS.cZa is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, dsh^UAS.cAa

(Waldrop et al., 2006)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/nkd^UAS.cZa is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, dsh^ΔbPDZ.UAS

(Waldrop et al., 2006)

Scer\GAL4^e22c/nkd^UAS.cZa is a non-suppressor of lethal | recessive | embryonic stage phenotype of tum^AR2

(Jones and Bejsovec, 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF/nkd^UAS.cZa is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, dsh^UAS.cAa

(Waldrop et al., 2006)

Scer\GAL4^GMR.PF/nkd^UAS.cZa is a suppressor of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, Xlae\csnk1e^KD.UAS

(Zhang et al., 2006)

Scer\GAL4^e22c/nkd^UAS.cZa is a suppressor of embryonic/first instar larval cuticle phenotype of tum^AR2

(Jones and Bejsovec, 2005)

Scer\GAL4^e22c/nkd^UAS.cZa is a suppressor of denticle phenotype of tum^AR2

(Jones and Bejsovec, 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^arm.PS/nkd^UAS.cZa is a non-suppressor of abdominal ventral denticle belt phenotype of Scer\GAL4^arm.PS, arm^{S10.UAS.Tag:MYC}

(Jones et al., 2010)

Scer\GAL4^GMR.PF/nkd^UAS.cZa is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, dsh^ΔbPDZ.UAS

(Waldrop et al., 2006)

Scer\GAL4^e22c/nkd^UAS.cZa is a non-suppressor of embryonic epidermis & nucleus phenotype of tum^AR2

(Jones and Bejsovec, 2005)

Other

Statement

Reference

Scer\GAL4^e22c, nkd^UAS.cZa, wg[+]/wg^l-17 has embryonic/first instar larval cuticle phenotype

(Jones and Bejsovec, 2005)

Scer\GAL4^e22c, nkd^UAS.cZa, wg^l-17 has embryonic/first instar larval cuticle phenotype

(Jones and Bejsovec, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of nkd^Scer\UAS.cZa driven by Scer\GAL4^arm.PS is unable to block the cell-fate transformation induced by arm^{S10.Scer\UAS.T:Hsap\MYC} expression.

(Jones et al., 2010)

Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c in a wg^l-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50C^AR2; the triple mutant embryos have a wild-type cuticle pattern. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c significantly rescues the cuticle pattern defects seen in RacGAP50C^AR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50C^AR2 embryos is unaffected if the embryos are also expressing nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c.

(Jones and Bejsovec, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/nkd^UAS.cZa rescues nkd³/nkd²

(Waldrop et al., 2006)

Scer\GAL4^da.G32/nkd^UAS.cZa rescues nkd³/nkd²

(Rousset et al., 2002)

Partially rescues

Scer\GAL4^en-e16E/nkd^UAS.cZa partially rescues nkd³

(Chan et al., 2007)

Scer\GAL4^ptc-559.1/nkd^UAS.cZa partially rescues nkd³

(Chan et al., 2007)

Comments

The naked cuticle phenotype of nkd³ embryos is partially rescued by expression of nkd^Scer\UAS.cZa under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^en-e16E, with more complete rescue being seen when Scer\GAL4^ptc-559.1 is used as the driver.

(Chan et al., 2007)

Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^da.G32 rescues the cuticle defects of nkd²/nkd³ embryos.

(Waldrop et al., 2006)

Rescues the cuticle defects of nkd²/nkd³ embryos when expressed under the control of Scer\GAL4^da.G32.

(Rousset et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25382

w^*; P{UAS-nkd.Z}2; P{lwB}hh^H90/TM3, P{HB-lacZ}GS2, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

nkd^Scer\UAS.cZa

nkd^UAS.cZa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Zeng

(Zeng et al., 2000)

Secondary FlyBase IDs

References (11)

Report Sections

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