FlyBase Allele Report: Dmel\nkd[hs.Tag:MYC]

General Information

Symbol

Dmel\nkd^hs.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0104470

Feature type

allele

Associated gene

Dmel\nkd

Associated Insertion(s)

Carried in Construct

P{hs-nkd.myc}

Key Links

Transgenic product class

cDNA

(Zeng et al., 2000)

wild_type

(Zeng et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zeng et al., 2000)

wild_type

(Zeng et al., 2000)

Mutagen

in vitro construct

(Zeng et al., 2000)

Progenitor genotype

Carried in construct

P{hs-nkd.myc}

Cytology

Description

An Hsp70 promoter drives expression of a nkd cDNA fragment (that lacks 5' and 3' untranslated regions) that is tagged at the carboxy-terminal end with Tag:MYC. An Hsp70 3' untranslated region is also present.

(Zeng et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

nkd

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Zeng et al., 2000)

Phenotype Manifest In

ventral denticle belt

(Zeng et al., 2000)

Detailed Description

Statement

Reference

Expression of nkd^{hs.T:Hsap\MYC} in otherwise wild-type embryos using heat shock occasionally produces weak denticle belt fusion phenotypes.

(Zeng et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

nkd^hs.Tag:MYC, wg^l-12/wg[+] has ventral denticle belt phenotype

(Zeng et al., 2000)

nkd^hs.Tag:MYC, wg^l-12/wg[+] has denticle row 5 | ectopic phenotype

(Zeng et al., 2000)

nkd^hs.Tag:MYC, wg^l-12 has ventral denticle belt phenotype

(Zeng et al., 2000)

nkd^hs.Tag:MYC, wg^l-12 has denticle row 5 | ectopic phenotype

(Zeng et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygous wg^l-12 embryos kept at the restrictive temperature are patterned normally. Heterozygous wg^l-12 embryos also expressing nkd^{hs.T:Hsap\MYC} (using heat shock) secrete cuticles with denticle belt fusions and an excess of type 5 denticles. Homozygous wg^l-12 embryos kept at the restrictive temperature have a uniform lawn of type 5 denticles and are unaffected by expression of nkd^{hs.T:Hsap\MYC}.

(Zeng et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

nkd^hs.Tag:MYC rescues nkd²

(Zeng et al., 2000)

nkd^hs.Tag:MYC rescues nkd³

(Zeng et al., 2000)

Comments

Rescues the nkd³ cuticle phenotype when expressed using heat shock. The strongest rescue is seen when 2-4 hour embryos are heat shocked for 15 minutes at 37^oC.

(Zeng et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25381

y¹ w^*; P{hs-nkd.myc}9

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nkd^{hs.T:Hsap\MYC}

nkd^hs.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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