Amino acid replacement: G170R.
G15000907A
G945A
G169S | Prosbeta2-PA
G170R
Difference between reported amino acid substitution and FlyBase curator due to differences in strain sequence. Authors sequence has G170 encoded by GGA while FlyBase sequence is GGT.
Brains of heterozygous larvae raised at the restrictive temperature of 29oC show an increase in the incidence of tetraploid and aneuploid metaphase figures, although more than 95% of mitotic spreads are normal. A few mitotic figures show precocious chromatid separation. The mean mitotic index is somewhat reduced compared to controls, probably reflecting the slower developmental rate of the mutant larvae.
Heterozygotes shifted to the restrictive temperature for 48 hours during the third larval instar survive to adulthood and have a mild extra vein phenotype in the wing.
Heterozygous pupae heat pulsed between 7 and 23 hour APF give rise to pharate adults that do not emerge but show specific bristle defects. A small number of double socket sense organs appear on the notum, abdomen and wing margin. Sheath cells are occasionally duplicated. The shaft cell is occasionally transformed into a second socket cell. pros expression indicates that microchaetae occasionally show a neuron to sheath transformation. Longer heat pulses cause earlier lethality.
Heterozygotes show no apparent phenotype when raised at 25oC. At 29oC, heterozygous larvae develop slowly and many of them fail to shorten at pupariation, resulting in elongated and sometimes curved pupae. Metamorphosis is abnormal; the imaginal disc derivatives are grossly underdeveloped and there is a complete lack of adult abdomen structures. Homozygotes die as larvae when raised at temperatures of 18 to 29oC. Hemizygotes usually die soon after hatching, although at 18oC some survive to the late larval or early pupal stage.
Dies at 29oC, viable at room temperature.
Females are almost sterile at 22oC. Has a dominant Minute phenotype. Heterozygotes die in the puparium at 29oC. Dead late pupae which have completed the development of the imago are seen in addition to early pupae in which extensive histolysis has occurred. The temperature-sensitive period immediately precedes the lethal phase, and is in the late third instar to pupal stage. Prosβ21 Pros261 flies do not survive at 17 or 22oC.
Lethal phase is during the pupal stage at 29oC.
Prosβ21/Prosbeta2[+], Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]
Prosβ21, Pros26[+]/Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]
Prosβ21 has lethal | dominant | heat sensitive phenotype, suppressible by Prosα2SuDTS/Su(DTS)-1[+]
Prosβ21 has lethal | dominant | heat sensitive phenotype, suppressible by Df(3R)T-32/+
Prosβ21, Prosβ61 has lethal | dominant | larval stage phenotype, suppressible | partially by Prosα2SuDTS/Su(DTS)-1[+]
Prosβ21/Prosbeta2[+] is a non-suppressor of lethal | recessive phenotype of Prosα2SuDTS
Prosα2SuDTS/Su(DTS)-1[+], Prosβ21 has viable phenotype
Prosβ21, Pros26[+]/Prosβ61 has lethal | dominant | larval stage phenotype
Prosβ21/Prosbeta2[+], Prosβ61 has lethal | dominant | larval stage phenotype
Prosβ21, Prosβ61.UAS, Scer\GAL4sca-537.4 has lethal phenotype
Prosβ21, Prosβ61 has lethal | dominant | larval stage phenotype
Prosβ21 has macrochaeta phenotype, enhanceable by H[+]/HE31
Prosβ21 has microchaeta phenotype, enhanceable by H[+]/HE31
Prosβ21 is an enhancer of wing phenotype of Mmus\Cul1UAS.cHa, Scer\GAL4Bx-MS1096
Prosβ21/Prosbeta2[+] is an enhancer of macrochaeta phenotype of HE31
Prosβ21/Prosbeta2[+] is an enhancer of microchaeta phenotype of HE31
Prosβ21 is an enhancer of macrochaeta phenotype of Nicd.hs
Prosβ21 is an enhancer of tormogen cell | ectopic phenotype of Nicd.hs
Pros26rv10e shows no synthetic lethal interaction with Prosβ21.
Prosβ21 Pros25SuDTS double heterozygotes are viable at 29oC, in contrast to Prosβ21 single heterozygotes.
Pros261 Prosβ21 double heterozygotes show early larval lethality at 25oC, which is partially suppressed by Pros25SuDTS/+.
The constitutive extra vein phenotype in the wing caused by the P{UAS-vn.S}1.1 insertion in the absence of an Scer\GAL4 driver is enhanced in combination with Prosβ21 and following a shift to the restrictive temperature.
Prosβ21 shows synthetic non-conditional lethality with Pros261.Scer\UAS, Scer\GAL4sca-537.4. Double heterozygotes of HE31 with Pros261 or Prosβ21 show strong synergistic enhancement of the double socket bristle and microchaete phenotypes. The mild double socket phenotype of Nicd.hs is enhanced by Pros261 and Prosβ21.
The Prosβ21 dominant temperature sensitive lethal effect is dominantly suppressed by Su(DTS)-11. Pros261 and Prosβ21 are lethal in transheterozygous condition, even at normally permissive temperatures. The synthetic lethal period occurs during early larval development, in contrast to the dominant temperature sensitive lethal period of either allele which occurs during the late pupal stage. This synthetic lethal effect is partially suppressed by Su(DTS)-11; a small fraction of + Pros261 Su(DTS)-11/Prosβ21 + + animals survive to adulthood at 18oC.
Reducing proteasome function by introduction of Prosβ21 to Scer\GAL4Bx-MS1096/Mmus\Cul1Scer\UAS.cHa flies enhances the wing size phenotype.
Prosβ21 is rescued by Prosβ2+t2.7
