FB2024_03 , released June 25, 2024
Allele: Dmel\Prosβ61.UAS
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General Information
Symbol
Dmel\Prosβ61.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0101587
Feature type
allele
Associated gene
Dmel\Prosβ6
Associated Insertion(s)
Carried in Construct
P{UAS-DTS5.S}
Also Known As
UAS-DTS5, UAS-DTS5-11
Key Links
Transgenic product class
missense_variant
(Schweisguth, 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Schweisguth, 1999)
Mutagen
in vitro construct
(Schweisguth, 1999)
Progenitor genotype
Prosβ61
(Schweisguth, 1999)
Carried in construct
P{UAS-DTS5.S}
Cytology
Description

Expression of a 1.2kb BamHI-NheI genomic fragment including the Pros261 allele is governed by UAS regulatory sequences.

(Schweisguth, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Prosβ6
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Pros261.Scer\UAS using Scer\GAL4GMR.PF at 29[o]C results in considerable disorganization of ommatidial units in larval eye discs.

      (Mallik and Lakhotia, 2010)

      Expression of Pros261.Scer\UAS under the control of Scer\GAL4GMR.PF does not result in a rough eye phenotype.

      (Shulman and Feany, 2003)

      Expression of Pros261.Scer\UAS in the eye with Scer\GAL4GMR.PF causes a mild rough eye phenotype ad occasional loss of pigmentation in approximately 20% of flies.

      (Chan et al., 2002)

      When expression is driven by two copies of Scer\GAL4sca-537.4, temperature sensitive lethality results. Flies die as pharate adults showing a strong multiple socket phenotype. When expression is driven by one copy of Scer\GAL4sca-537.4 at 29oC from 0 to 24hr APF the majority of sense organs appear to be wild type. Those sense organs that are mutant show double socket or sheath cells.

      (Schweisguth, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Prosβ21 shows synthetic non-conditional lethality with Pros261.Scer\UAS, Scer\GAL4sca-537.4.

      (Schweisguth, 1999)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression.

      Co-expression of Pros261.Scer\UAS via Scer\GAL4elav.PU does not significantly affect the Hsp60DdsRNA.Sym.Scer\UAS-mediated partial suppression of the eye degeneration phenotype caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression.

      The eye degeneration caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is enhanced by co-expression of Pros261.Scer\UAS.

      The eye degeneration caused by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 overexpression via Scer\GAL4GMR.PU is enhanced by co-expression of Pros261.Scer\UAS.

      (Arya et al., 2010)

      Co-expression of Pros261.Scer\UAS suppresses the ability of Hsap\MJDfl.Q27.Scer\UAS.T:Hsap\MYC to restore the eye and retina phenotypes towards normal in flies expressing Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF, such that the triple mutant flies have a reduction in eye pigmentation, show collapse of the eye and loss of internal retinal tissue.

      (Warrick et al., 2005)

      The rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4GMR.PF is not modified if the flies are also carrying Pros261.Scer\UAS.

      (Shulman and Feany, 2003)

      Co-expression of Pros261.Scer\UAS with Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 (both under the control of Scer\GAL4GMR.PF) enhances the eye degeneration found with expression of Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 alone. Analysis of protein extracts reveals that the enhancement of degneration is associated with a change in the solubility properties of the Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 protein.

      Co-expression of Pros261.Scer\UAS and Hsap\HSPA1LScer\UAS.cWa, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) suppress the eye phenotype.

      Co-expression of Pros261.Scer\UAS and Hsc70-4K71S.Scer\UAS, together with the pathogenic Hsap\ARQ112.Scer\UAS.T:Ivir\HA1 lines (all under the control of the Scer\GAL4GMR.PF driver) enhances the eye phenotype.

      (Chan et al., 2002)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pros261.Scer\UAS
      Prosβ61.Scer\UAS
      Prosβ61.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (10)