FlyBase Allele Report: Mmus\Cul1[UAS.cHa]

General Information

Symbol

Mmus\Cul1^UAS.cHa

Species

M. musculus

Name

FlyBase ID

FBal0299005

Feature type

allele

Associated gene

Mmus\Cul1

Associated Insertion(s)

Carried in Construct

P{UAS-mCul1.H}

Transgenic product class

wild_type

(Heriche et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Heriche et al., 2003)

Mutagen

in vitro construct

(Heriche et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-mCul1.H}

Cytology

Description

UASt regulatory sequences drive expression of Mmus\Cul1 coding sequence.

(Heriche et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Cul1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

wing hair | ectopic, with Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Detailed Description

Statement

Reference

Expression of Mmus\Cul1^Scer\UAS.cHa in the third instar larval wing imaginal disc, under the control of Scer\GAL4^Bx-MS1096 leads to a strong reduction in the size of the wing without any gross patterning defects. This is more severe in males than females, due to dosage compensation of Scer\GAL4^Bx-MS1096 levels. Increasing transgene copy reduces the wing to a rudimentary stump. Reduced wings occasionally show a multiple wing hair phenotype in which several trichomes instead of just one are produced by individual intervein cells, particularly in the region near the anterior crossvein.

Wing hair density is unchanged by expression of Mmus\Cul1^Scer\UAS.cHa under the control of Scer\GAL4^Bx-MS1096, even when there is a dramatic effect on wing size.

(Heriche et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by slmb^e4-1

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by slmb^P245

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Cul1^k01207/Cul1[+]

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Df(2R)NCX8/+

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Prosβ2¹

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Prosβ6¹

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing | heat sensitive phenotype, enhanceable by E2f1^hs.PD

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, enhanceable by Df(3R)e-R1

(Heriche et al., 2003)

Suppressed by

Statement

Reference

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096, slmb^e4-1 has wing phenotype, suppressible by E2f1⁰⁷¹⁷²

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096, slmb^P245 has wing phenotype, suppressible by E2f1⁰⁷¹⁷²

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing hair | ectopic phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by T(1;Y)B106

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible | partially by SkpA^UAS.cHa, Scer\GAL4^Bx-MS1096

(Heriche et al., 2003)

Mmus\Cul1^UAS.cHa, Scer\GAL4^Bx-MS1096 has wing phenotype, suppressible by E2f1[+]/E2f1⁹¹

(Heriche et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa rescues the reduction in wing size seen in Mmus\Cul1^Scer\UAS.cHa Scer\GAL4^Bx-MS1096, indicating that Mmus\Cul1^Scer\UAS.cHa induces elevated apoptosis. BacA\p35^Scer\UAS.cHa expression also suppresses the multiple wing hair phenotype.

Co-expression of Cul1^Scer\UAS.cHa with Mmus\Cul1^Scer\UAS.cHa in the third instar larval wing imaginal disc, under the control of Scer\GAL4^Bx-MS1096 suppresses the wing reduction phenotype induced by Mmus\Cul1^Scer\UAS.cHa overexpression alone.

Introduction of a single copy of Cul1^k01207 or Df(2R)NCX8 dominantly enhances the wing reduction phenotype in Mmus\Cul1^Scer\UAS.cHa Scer\GAL4^Bx-MS1096 mutants.

A T(1;Y)B106 background (where SkpA and Roc1a are deleted) strongly enhances the reduction in wing size seen in Scer\GAL4^Bx-MS1096/Mmus\Cul1^Scer\UAS.cHa mutants.

Co-expression of SkpA^Scer\UAS.cHa with Scer\GAL4^Bx-MS1096/Mmus\Cul1^Scer\UAS.cHa partially restores wing size.

Reducing proteasome function by introduction of Prosβ2¹ to Scer\GAL4^Bx-MS1096/Mmus\Cul1^Scer\UAS.cHa flies enhances the wing size phenotype.

Reducing proteasome function by introduction of Prosβ6¹ to Scer\GAL4^Bx-MS1096/Mmus\Cul1^Scer\UAS.cHa flies enhances the wing size phenotype.

A E2f1⁹¹/+ background rescues wing size to wild-type in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants.

Increased levels of E2f1 through expression of E2f1^hs.PD enhances the small wing phenotype in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants. This enhancement is stronger and completely penetrant when exposed to heat shock treatment every 24 hours during third instar and early pupal development.

A Df(3R)e-R1 background enhances the small wing phenotype found in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants.

A slmb^e4-1 background enhances the small wing phenotype found in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants. The presence of E2f1⁰⁷¹⁷² as well as slmb^e4-1 reverses the enhancement of Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 by a slmb^e4-1 background.

A slmb^P245 background enhances the small wing phenotype found in Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 over-expression mutants. The presence of E2f1⁰⁷¹⁷² as well as slmb^P245 reverses the enhancement of Mmus\Cul1^Scer\UAS.cHa/Scer\GAL4^Bx-MS1096 by a slmb^P245 background.

(Heriche et al., 2003)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Cul1^Scer\UAS.cHa

Mmus\Cul1^UAS.cHa

Name Synonyms

Secondary FlyBase IDs

References (1)

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