FlyBase Allele Report: Dmel\fz[25]

General Information

Symbol

Dmel\fz²⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0004941

Feature type

allele

Associated gene

Dmel\fz

Associated Insertion(s)

Carried in Construct

Also Known As

fz^R54

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Polytene chromosomes normal.

Description

Amino acid replacement: M469R. Amino acid replacement: W500R.

(Povelones et al., 2005)

Amino acid replacement: M469R. Amino acid replacement: W500R. Nucleotide substitution: G for T. Nucleotide substitution: C for T.

(Jones et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:14,366,940..14,366,940 [+]

Nucleotide change:

T14366940G

Reported nucleotide change:

T?G

Amino acid change:

M469R | fz-PA; M469R | fz-PC

Reported amino acid change:

M469R

Comment:

One of two amino acid substitutions in mutant.

(Povelones et al., 2005, Jones et al., 1996)

point_mutation

3L:14,367,032..14,367,032 [+]

Nucleotide change:

T14367032C

Reported nucleotide change:

T?C

Amino acid change:

W500R | fz-PA; W500R | fz-PC

Reported amino acid change:

W500R

Comment:

One of two amino acid substitutions in mutant.

(Povelones et al., 2005, Jones et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

external sensory organ precursor cell I

(Gho and Schweisguth, 1998)

eye photoreceptor cell | somatic clone

(Strutt et al., 2002)

leg

(Strutt et al., 2012)

leg joint | ectopic

(Strutt et al., 2012)

ommatidium | somatic clone

(Strutt et al., 2002)

sensory mother cell

(Roegiers et al., 2001)

spindle

(Roegiers et al., 2001)

wing & microchaeta

(Adler et al., 1997)

wing | cell non-autonomous | somatic clone

(Adler et al., 2000)

wing hair

(Taylor et al., 1998, Turner and Adler, 1995)

wing hair (with fz¹⁵)

(Strutt and Strutt, 2002)

wing hair | cell non-autonomous | somatic clone

(Strutt and Strutt, 2002)

wing hair | increased number (with fz³⁰)

(Povelones et al., 2005)

wing hair | somatic clone

(Strutt and Strutt, 2002)

Detailed Description

Statement

Reference

Mutant adults have an average of 2.8 +/- 0.4 extra tarsal joints in the leg compared to wild type.

(Strutt et al., 2012)

When mutant clones are made in the developing eye, no R-cell precursor nuclear migration phenotype was seen.

(Houalla et al., 2005)

fz²⁵ has a moderate multiple wing hair phenotype in combination with fz³⁰.

(Povelones et al., 2005)

The wings of fz¹⁵/fz²⁵ animals have a trichome swirling pattern that is distinct from that due to ds^33k/ds^UAO71. In cells surrounding somatic clones of fz²⁵ in the wing, trichomes point inwards, towards the clone, and form on cell edges. Ommatidia in the eyes of fz¹⁵/fz²⁵ flies have randomised dorsal-ventral and anterior-posterior polarity, and often exhibit randomised degrees of rotation.

(Strutt and Strutt, 2002)

26.8% of ommatidia in homozygous mutant somatic clones in the eye are normal. 28.6% have rotated ommatidia, 37% have chirality defects, 7.2% are achiral (0.3% unscorable).

(Strutt et al., 2002)

The pI division of sensory organ precursor cells in the pupal notum has a random orientation with respect to the anterior-posterior axis, in contrast to wild type. In 90% of cases the mitotic spindle positions itself perpendicular to the crescent of pon protein in these cells, regardless of the relationship of the crescent to the anterior-posterior axis. The spindles have an abnormal curved morphology throughout mitosis. There is no effect on the proper apical-basal orientation of the pIIb division.

(Roegiers et al., 2001)

Homozygous somatic clones in the wing cause domineering non-autonomy.

(Adler et al., 2000)

Dendritic development in homozygous mutants is indistinguishable from wild-type.

(Gao et al., 2000)

Mutants exhibit randomly oriented pI divisions in the epithelial plane. The socket, shaft and pIIb cells are always localised in the region of the cell in contact with pIIb. The socket cells always occupies an eccentric position.

(Gho and Schweisguth, 1998)

Clones in adult wings cause wild type hairs distal to and in part anterior and posterior to the clone displaying abnormal polarity have a strong bias to point towards, rather than away from the clone. Clones in differentiating pupal wings show the same phenotype. This is referred to as an 'attractive' non-cell-autonomous effect. Wild type hairs at the proximal edge of the clone are usually rescued.

(Adler et al., 1997)

Strong fz phenotype.

(Jones et al., 1996)

Dissected pupal wings can be cultured in vitro. Wings develop an adult pattern over the same time course as wild-type wings. Prehairs typically form in the central regions of the wing cells, mimicking what is seen for wings in situ.

(Turner and Adler, 1995)

strong thoracic bristle phenotype strong wing-hair disorientation

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

fz²⁵ has abnormal planar polarity | cell non-autonomous phenotype, enhanceable by pk^D

(Adler et al., 2000)

Other

Statement

Reference

fz2^C1, fz²⁵ has visible | somatic clone phenotype

(Strutt et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

fz²⁵ has wing | cell non-autonomous | somatic clone phenotype, enhanceable by pk^D

(Adler et al., 2000)

Enhancer of

Statement

Reference

fz²⁵ is an enhancer of wing hair phenotype of sha^VB13

(Ren et al., 2006)

fz³⁰/fz²⁵ is an enhancer of wing hair phenotype of fy¹/fy⁶

(Lee and Adler, 2002)

NOT Enhancer of

Statement

Reference

fz²⁵ is a non-enhancer of ommatidium phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz²⁵ is a non-enhancer of photoreceptor cell R3 | ectopic phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz²⁵ is a non-enhancer of photoreceptor cell R4 phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

Suppressor of

Statement

Reference

fz²⁵ is a suppressor of wing hair phenotype of Vang^A3

(Taylor et al., 1998)

fz²⁵ is a suppressor of wing hair phenotype of Vang^Tbs42

(Taylor et al., 1998)

NOT Suppressor of

Statement

Reference

fz[+]/fz²⁵ is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Fiehler and Wolff, 2008)

fz²⁵ is a non-suppressor of ommatidium phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz²⁵ is a non-suppressor of photoreceptor cell R3 | ectopic phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

fz²⁵ is a non-suppressor of photoreceptor cell R4 phenotype of Scer\GAL4^sev.PM181, stan^UAS.cUa

(Das et al., 2002)

Other

Statement

Reference

fz2^C1, fz²⁵ has wing margin bristle | somatic clone phenotype

(Strutt et al., 2012)

fz2^C1, fz²⁵ has wing margin | somatic clone phenotype

(Strutt et al., 2012)

fz2^e2, fz^GL31/fz²⁵ has embryonic/first instar larval cuticle | maternal effect phenotype

(Povelones et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

fz²⁵ fz2^C1 double homozygous clones in the wing result in notching of the wing margin and loss of margin bristles in the mutant tissue.

(Strutt et al., 2012)

Embryos that lack zygotic fz and fz2 function which are derived from fz²⁵/fz^GL31 fz2^e2 females mated to fz^GL31 fz2^e2/+ males are modestly shortened and have mild patterning defects in the cuticle.

(Povelones et al., 2005)

The proportion of symmetrical (R3/R3) ommatidia in the eyes of stan^Scer\UAS.cUa; Scer\GAL4^sev.PM181 flies is unaffected by heterozygosity for fz²⁵.

(Das et al., 2002)

fz²⁵ clones in a pk^D background show significantly stronger domineering non-autonomy than in a wild-type background.

(Adler et al., 2000)

In double mutant combinations between Vang^A3, Vang^A5 and Vang^Tbs42 with fz¹, fz²⁵ and fz³⁰, the fz-in type of polarity pattern was slightly less abnormal than in either single mutant. The domineering nonautonomy of Vang^A3 and Vang^Tbs42 clones can be suppressed by fz²⁵ and fz³⁰.

(Taylor et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

fz¹⁵/fz²⁵ is rescued by fz^Act5C.EYFP

(Strutt and Strutt, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Adler.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

(Weber et al., 2012)

R54

(Weber et al., 2012)

fz²⁵

fz^R54

(Strutt et al., 2012, Fiehler and Wolff, 2008, Ren et al., 2006, Povelones et al., 2005, Das et al., 2002, Lee and Adler, 2002, Schaefer et al., 2001, Adler et al., 2000, Cooper and Bray, 1999, Adler et al., 1998, Taylor et al., 1998, Adler et al., 1997, Jones et al., 1996, Turner and Adler, 1995, Adler, 1992)

fz^r54

(Roegiers et al., 2001, Gao et al., 2000, Lu et al., 1999, Gho and Schweisguth, 1998)

Name Synonyms

strong fz

(Strutt et al., 2002)

Secondary FlyBase IDs

References (27)

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