FlyBase Gene Report: Dmel\Vmat

General Information

Symbol

Dmel\Vmat

Species

D. melanogaster

Name

Vesicular monoamine transporter

Annotation Symbol

CG33528

Feature Type

protein_coding_gene

FlyBase ID

FBgn0260964

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

Vesicular monoamine transporter (Vmat) encodes a protein responsible for packaging the neurotransmitters dopamine, serotonin and octopamine into secretory vesicles. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dVMAT, DVMAT-A, l(2)SH0459

Key Links

Genomic Location

Cytogenetic map

50A14-50B1

Sequence location

2R:13,513,132..13,534,251 [-]

Recombination map

2-68

RefSeq locus

NT_033778 REGION:13513132..13534251

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables monoamine transmembrane transporter activity

inferred from direct assay

(Greer et al., 2005)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables serotonin:sodium:chloride symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000607052

(GO Reference Genome Project, 2011-)

enables transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR011701, InterPro:IPR020846

(InterPro Project Members, 2004-)

enables xenobiotic transmembrane transporter activity

inferred from electronic annotation with InterPro:IPR004734

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in aminergic neurotransmitter loading into synaptic vesicle

inferred from direct assay

(Greer et al., 2005)

involved_in dopamine transport

inferred from mutant phenotype

(Sang et al., 2007)

involved_in histamine transport

inferred from mutant phenotype

(Romero-Calderón et al., 2008)

involved_in monoamine transport

inferred from direct assay

(Greer et al., 2005)

involved_in positive regulation of dopaminergic neuron differentiation

inferred from mutant phenotype

(Lawal et al., 2010)

involved_in response to rotenone

inferred from mutant phenotype

(Lawal et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in aminergic neurotransmitter loading into synaptic vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000607052

(GO Reference Genome Project, 2011-)

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR011701

(InterPro Project Members, 2004-)

involved_in xenobiotic transport

inferred from electronic annotation with InterPro:IPR004734

(InterPro Project Members, 2004-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in neuromuscular junction

inferred from direct assay

(Greer et al., 2005)

located_in synaptic vesicle

inferred from direct assay

(Grygoruk et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR004734

(InterPro Project Members, 2004-)

is_active_in synaptic vesicle membrane

inferred from biological aspect of ancestor with PANTHER:PTN000607052

(GO Reference Genome Project, 2011-)

is_active_in terminal bouton

inferred from biological aspect of ancestor with PANTHER:PTN000607052

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC18 FAMILY OF VESICULAR NEUROTRANSMITTER TRANSPORTERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

serotonin:sodium:chloride symporter activity
RHEA 51196:

Summaries

Gene Snapshot

Vesicular monoamine transporter (Vmat) encodes a protein responsible for packaging the neurotransmitters dopamine, serotonin and octopamine into secretory vesicles. [Date last reviewed: 2019-03-21]

Gene Group (FlyBase)

SLC18 FAMILY OF VESICULAR NEUROTRANSMITTER TRANSPORTERS -: Solute carrier family 18 (SLC18) members are vesicular neurotransmitter transmembrane transporters for the biogenic amines and acetylcholine. (Adapted from FBrf0225369).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Vmat for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8IH57)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Vmat-RC

FBtr0091493

NM_001014524

2470

610

Vmat-RD

FBtr0091492

NM_001014525

2446

646

Vmat-RE

FBtr0091491

NM_001014526

7325

610

Vmat-RF

FBtr0310062

NM_001274027

7141

610

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Vmat-PC

FBpp0090963

65.6

610

4.82

FBpp0090962

68.7

646

4.74

FBpp0090961

65.6

610

4.82

FBpp0301763

65.6

610

4.82

Q86NW1

NP_001260956

AGB93488

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

610 aa isoforms: Vmat-PC, Vmat-PE, Vmat-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Vmat using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.80

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ventral midline of embryo

(Wheeler et al., 2009)

embryonic stage 13

larval VUM motor neuron

(Wheeler et al., 2006)

embryonic stage 14

larval H-cell

(Stagg et al., 2011)

larval VUM motor neuron

(Stagg et al., 2011)

embryonic stage 17

ventral midline of embryo | restricted

(Greer et al., 2005)

larval ventral nerve cord | dorsal | restricted

(Greer et al., 2005)

larval VUM motor neuron

(Wheeler et al., 2006)

larval H-cell

(Wheeler et al., 2006)

adult stage

fenestrated glial cell

(Romero-Calderón et al., 2008)

lamina | distal

(Romero-Calderón et al., 2008)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

organism

(Greer et al., 2005)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

Comment: Transcripts encoding both Vmat protein isoforms are expressed: isoform A (Vmat-RC,RE and RF) and B (Vmat-RD).

(Greer et al., 2005)

Additional Descriptive Data

Vmat is expressed in the H-cell and in three VUM neurons.

(Stagg et al., 2011)

An isoform of Vmat with a retained 3' intron is expressed in a layer between the retina and the lamina in the adult brain.

(Romero-Calderón et al., 2008)

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

Transcripts encoding both Vmat protein isoforms are detected in clusters of cells in the embryonic ventral nerve cord. These include unpaired neurons at the midline of each segment, bilateral cells dorsal and posterior to each of these unpaired neurons, and more dorsally located cells.

(Greer et al., 2005)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

ventral midline of embryo | restricted

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

larval ventral nerve cord | dorsal | restricted

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

third instar larval stage

larval serotonergic SP1 neuron

(Greer et al., 2005)

larval serotonergic SP2 neuron

(Greer et al., 2005)

larval ventral nerve cord | dorsal | restricted

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

ventral midline of embryo | restricted

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

embryonic/larval brain | restricted

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

dopaminergic neuron | subset of embryonic/larval brain

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

larval serotonergic LP1 neuron

(Greer et al., 2005)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

fenestrated glial cell

(Romero-Calderón et al., 2008)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

Comment: Antibody specific to Vmat isoform A (Vmat-PC,PE and PF).

(Greer et al., 2005)

Additional Descriptive Data

Vmat labels the fenestrated glia layer located between the lamina and the retina.

(Damulewicz and Pyza, 2011)

Vmat protein is present in a punctate pattern in the cell body, and in the synapses of the cell processes of neurons in the central brain.

(Wang et al., 2011)

An antibody directed against an isoform of Vmat containing a retained 3' intron detects expressed in a thin layer between the retina and the lamina in the adult brain.

(Romero-Calderón et al., 2008)

Vmat protein is detected in a subset of the neurons in the dorsomedial (DM) and dorsolateral (DL1) dopaminergic clusters. Expression is also detected in a large number of dopaminergic neurons in the medulla. Vmat protein localises to a lateral protocerebrum serotonergic cluster, that projects to the medulla, lobula and lamina. In the medulla, expression is detected in medulla layers M1, M2, M4, M8-M10; it does not colocalise with the terminals of the photoreceptors R7 and R8 or the monopolar cells L1 and L2. In the central brain, Vmat protein is expressed in the serotonergic neurons that innervate the fan-shaped body.

(Chang et al., 2006)

Vmat protein isoform A (Vmat-PC,PE and PF) is detected in clusters of cells in the embryonic and larval ventral nerve cord. These include unpaired neurons at the midline of each segment, bilateral cells dorsal and posterior to each of these unpaired neurons, and more dorsally located cells. Expression of Vmat-A isoform is also detected in clusters of serotonergic and dopaminergic neurons in the larval brain.

(Greer et al., 2005)

Marker for

fenestrated glial cell

(Damulewicz and Pyza, 2011)

Subcellular Localization

CV Term

Evidence

References

located_in neuromuscular junction

inferred from direct assay

(Greer et al., 2005)

located_in synaptic vesicle

inferred from direct assay

(Grygoruk et al., 2010)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.0}Vmat^{MI07680-TG4.0}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult aminergic neuron

(Konstantinides et al., 2018)

adult octopaminergic neuron | subset

(Allen et al., 2020)

adult dopaminergic neuron | subset

(Allen et al., 2020)

adult serotonergic neuron | subset

(Allen et al., 2020)

adult VNC perineurial glial cell

(Allen et al., 2020)

Reporter: P{GMR76F01-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PPL1 neuron | subset

(Xie et al., 2018)

dopaminergic PPM3 neuron | subset

(Xie et al., 2018)

Reporter: P{GMR76F02-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PAL neuron | subset

(Xie et al., 2018)

dopaminergic PPL1 neuron | subset

(Xie et al., 2018)

dopaminergic PPL2ab neuron | subset

(Xie et al., 2018)

Reporter: P{GMR76F05-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

interneuron

(Clark et al., 2016)

third instar larval stage

larval mushroom body dopaminergic neuron f1

(Saumweber et al., 2018)

larval mushroom body dopaminergic neuron h1

(Saumweber et al., 2018)

larval mushroom body dopaminergic neuron i1

(Saumweber et al., 2018)

larval mushroom body dopaminergic neuron j1

(Saumweber et al., 2018)

larval mushroom body dopaminergic neuron k1

(Saumweber et al., 2018)

larval mushroom body input neuron m1

(Saumweber et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Vmat in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Vmat

View exonic expression by tissue for Dmel\Vmat

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346192/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260964

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259816

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260964

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33528

Flygut - An atlas of the Drosophila adult midgut

FBgn0260964

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 22 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Vmat

Transgenic constructs containing regulatory region of Vmat

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)CX1

(Simon et al., 2009)

Df(2R)MK2

(Simon et al., 2009)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Tub.PU

Vmat^KK112993

lethal, with Scer\GAL4^Ubi.PU

Vmat^KK112993

lethal (with Df(2R)CX1)

lethal | recessive

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Vmat^GD1982

viable, with Scer\GAL4^Mef2.PR

Vmat^GD1982

viable, with Scer\GAL4^pnr-MD237

Vmat^GD1982

viable, with Scer\GAL4^tin.CΔ4

Vmat^GD1982

Sterility

Allele

female semi-sterile, with Scer\GAL4^elav.PLu

Vmat^GD1982

female sterile

Vmat^Δ14

female sterile (with Df(2R)CX1)

female sterile (with Df(2R)MK2)

Vmat^SH0459

female sterile | recessive

Vmat^SH0459

male sterile

Vmat^Δ14

male sterile (with Df(2R)CX1)

male sterile (with Df(2R)MK2)

Vmat^SH0459

male sterile | recessive

Vmat^SH0459

Other Phenotypes

Allele

abnormal grooming behavior, with Scer\GAL4^Ddc.PL

Vmat^{UAS.Ex.Tag:HA}

abnormal grooming behavior, with Scer\GAL4^elav.PLu

Vmat^{UAS.Ex.Tag:HA}

abnormal locomotor behavior | adult stage

Vmat^SH0459

abnormal locomotor behavior | adult stage, with Scer\GAL4^Ddc.PL

Vmat^{UAS.Ex.Tag:HA}

abnormal locomotor behavior | larval stage

Vmat^SH0459

abnormal neurophysiology | larval stage

Vmat^SH0459

abnormal phototaxis | recessive

Vmat^SH0459

abnormal song, with Scer\GAL4^Ddc.PL

Vmat^{UAS.Ex.Tag:HA}

abnormal touch response | larval stage | recessive

Vmat^SH0459

short lived | dominant | female

Vmat^SH0459

short lived | male | recessive

Vmat^SH0459

Phenotype manifest in

Allele

dopaminergic PAL neuron | adult stage

Vmat^SH0459

dopaminergic PPL1 neuron | adult stage

Vmat^SH0459

embryonic/larval neuromuscular junction

Vmat^SH0459

oocyte

Vmat^SH0459

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (12)

Hsap\SLC18A1

13 of 14

Yes

Hsap\SLC18A2

12 of 14

Yes

5 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Slc18a1

13 of 14

Yes

Rnor\Slc18a2

13 of 14

Yes

5 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Slc18a2

13 of 14

Yes

Mmus\Slc18a1

12 of 14

Yes

Mmus\Slc18a3

6 of 14

Mmus\Slc18b1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\LOC733714

10 of 13

Yes

Xtro\slc18a1

10 of 13

Yes

Xtro\slc18a2

10 of 13

Yes

Xtro\LOC100495571

7 of 13

Xtro\LOC100496053

7 of 13

Yes

Xtro\LOC101732756

6 of 13

Yes

Xtro\LOC101731380

5 of 13

Xtro\slc18a3

4 of 13

Xtro\LOC100487393

1 of 13

Yes

Xtro\LOC100495897

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (18)

Drer\slc18a1

13 of 14

Yes

Drer\slc18a2

13 of 14

Yes

5 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\cat-1

13 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP004476

12 of 12

Yes

5 of 12

5 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\AT5G42210

1 of 13

Yes

Atha\G3Pp1

1 of 13

Yes

Atha\G3Pp2

1 of 13

Yes

Atha\PHT4;2

1 of 13

Yes

Atha\PHT4;3

1 of 13

Yes

Atha\STP4

1 of 13

Yes

Atha\ZIF1

1 of 13

Yes

Atha\ZIFL1

1 of 13

Yes

Atha\ZIFL2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\ESBP6

1 of 13

Yes

Scer\TPO1

1 of 13

Yes

Scer\TPO2

1 of 13

Yes

Scer\TPO3

1 of 13

Yes

Scer\VBA1

1 of 13

Yes

Scer\YCR023C

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\SPCC18.02

7 of 12

Yes

Spom\SPBC16A3.17c

1 of 12

Yes

Escherichia coli (enterobacterium) (9)

Ecol\garP

1 of 11

Yes

Ecol\hsrA

1 of 11

Yes

Ecol\lgoT

1 of 11

Yes

Ecol\mdfA

1 of 11

Yes

Ecol\mdtG

1 of 11

Yes

Ecol\mdtH

1 of 11

Yes

Ecol\shiA

1 of 11

Yes

Ecol\yajR

1 of 11

Yes

Ecol\ydeE

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Vmat. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (9)

VAChT

9 of 13

prt

7 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SLC18A2; solute carrier family 18 member A2

model of infantile parkinsonism-dystonia 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Vmat^{UAS.Ex.Tag:HA}

ameliorates Parkinson's disease 2

modeled by Hsap\PRKN^{T240R.UAS.Tag:MYC}

(Sang et al., 2007)

Vmat^RNAi.UAS

exacerbates Parkinson's disease 2

modeled by Hsap\PRKN^{T240R.UAS.Tag:MYC}

(Sang et al., 2007)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC18A1; solute carrier family 18 member A1

13 of 14

193002

SLC18A2; solute carrier family 18 member A2

12 of 14

193001

PARKINSONISM-DYSTONIA 2, INFANTILE-ONSET; PKDYS2

infantile parkinsonism-dystonia 2

SLC18A3; solute carrier family 18 member A3

5 of 14

600336

MYASTHENIC SYNDROME, CONGENITAL, 21, PRESYNAPTIC; CMS21

congenital myasthenic syndrome 21

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

MIST Molecular Interaction Search Tool

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

77910

DroID - A comprehensive database of gene and protein interactions.

FBgn0260964

MIST (protein-protein) - An integrated Molecular Interaction Database

3346192

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-68

Cytogenetic map

50A14-50B1

Sequence location

2R:13,513,132..13,534,251 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50A14-50B1

Limits computationally determined from genome sequence between P{lacW}drk^k13809&P{EP}mip120^EP606 and P{lacW}l(2)03105^k16702&P{lacW}fl(2)d^k16105

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

50B2-50B3

(Oh et al., 2003)

Experimentally Determined Recombination Data

Location

2-68

(FlyBase, 2016)

2-72.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24587

w¹¹¹⁸; Mi{ET1}Vmat^MB04557

31257

y¹ v¹; P{TRiP.JF01198}attP2

80342

y¹ v¹; P{TKO.GS03308}attP40

44471

y¹ v¹; P{TRiP.HMC02346}attP2

23020

y¹ w^67c23; Mi{ET1}Vmat^MB01214

33172

y¹ w^*; Mi{MIC}Vmat^MI02179/SM6a

FlyORF

F001377

M{UAS-Vmat.ORF}ZH-86Fb

F000952

M{UAS-Vmat.ORF.3xHA}ZH-86Fb

NIG-Fly

HMC02346

y¹ v¹; P{TRiP.HMC02346}attP2

VDRC

v104072

P{KK112993}VIE-260B

v4856

w¹¹¹⁸; P{GD1982}v4856

v318275

PBac{fTRG00922.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (62)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI07245

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0260964

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0260964

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Banerjee et al., 2021)

polyclonal

(Borycz et al., 2012, Romero-Calderón et al., 2008, Greer et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

1 allele of l(2)SH0459 recovered in a P-insertion screen.

(Oh et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Vmat CG33528

(Greer et al., 2005)

Source for merge of: Vmat l(2)SH0459

(Simon et al., 2009)

Source for merge of: CG6119 CG6139

(FlyBase Genome Annotators, 2005)

Additional comments

Annotations CG6119 and CG6139 merged as CG33528 in release 4.1 of the genome annotation.

(FlyBase Genome Annotators, 2005)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG33528

(National Institute of Genetics Fly Stocks, 2022-, Frenkel et al., 2017, Schertel et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Simon et al., 2009, Ni et al., 2008, Thimgan et al., 2006, Wheeler et al., 2006)

CG6119

(Simon et al., 2009, Romero-Calderón et al., 2008, Manh et al., 2005, Giot et al., 2003, Coulier et al., 2000)

CG6139

(Giot et al., 2003, Stempfl et al., 2002)

DVMAT

(Grygoruk et al., 2014, Curran and Chalasani, 2012, Damulewicz and Pyza, 2011, Fei et al., 2007, Sang et al., 2007, Middleton et al., 2006)

DVMAT-A

(Grygoruk et al., 2010, Chang et al., 2006)

DVMAT-B

(Borycz et al., 2012)

VMAT

(Naz and Siddique, 2021, Carvajal-Oliveros and Campusano, 2020, Jakšić et al., 2020, Sherer et al., 2020, Ni et al., 2019, Shiba-Fukushima et al., 2014, Lenz et al., 2013, Wu et al., 2013, Kaun et al., 2012, Muñoz-Soriano and Paricio, 2011, Wang et al., 2011, Seugnet et al., 2009, Romero-Calderón et al., 2008, Park et al., 2006, Wheeler et al., 2006)

Vmat

(Anthoney et al., 2023, Bossen et al., 2023, Rosikon et al., 2023, Voutyraki et al., 2023, Ceder and Fredriksson, 2022, Corrales et al., 2022, Velten et al., 2022, Baker et al., 2021, Ceder et al., 2021, Dulac et al., 2021, Jin et al., 2021, Zarubin et al., 2021, Aboukilila et al., 2020, Lathen et al., 2020, Brunet Avalos et al., 2019, Chu et al., 2019, Su, 2019, Tao et al., 2019, Croset et al., 2018, Transgenic RNAi Project members, 2017-, Crocker et al., 2016, Gene Disruption Project members, 2016-, Sarov et al., 2016, Williams et al., 2016, Dembeck et al., 2015, Gene Disruption Project members, 2015-, Gnerer et al., 2015, Bussell et al., 2014, DeSalvo et al., 2014, Williams et al., 2014, Harbison et al., 2013, Hong et al., 2012, Rodriguez et al., 2012, Stagg et al., 2011, Wheeler et al., 2009)

Vmt

(Subaraja et al., 2020)

l(2)SH0459

(Simon et al., 2009)

l(2)SH2 0459

(Oh et al., 2002.3.22)

vmat

(Babski et al., 2019, Kong et al., 2010)

Name Synonyms

CG33528

Drosophila vesicular monoamine transporter

(Simon et al., 2008)

Vesicular monoamine transporter

(Harbison et al., 2013)

Vmat

lethal (2) SH0459

vesicle transporter

(Daubert et al., 2010)

vesicular monoamine transporter

(Kaun et al., 2012, Muñoz-Soriano and Paricio, 2011, Seugnet et al., 2009, Park et al., 2007)

Secondary FlyBase IDs

FBgn0259816
FBgn0085016
FBgn0053528
FBgn0033849
FBgn0033852
FBtr0087689
FBpp0086808
FBtr0087690
FBpp0086809
FBtr0087691
FBpp0086810
FBtr0087692
FBpp0086811
FBgn0065773

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 65 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3346192

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8IH57

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8IH57

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3346192/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0260964

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0260964

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG33528

MARRVEL_MODEL - MARRVEL (model organism gene)

3346192

Linkouts

BioGRID - A database of protein and genetic interactions.

77910

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0260964

DroID - A comprehensive database of gene and protein interactions.

FBgn0260964

DRSC - Results frm RNAi screens

FBgn0260964

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0259816

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0260964

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG33528

Flygut - An atlas of the Drosophila adult midgut

FBgn0260964

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0260964

MIST (protein-protein) - An integrated Molecular Interaction Database

3346192

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (164)

The following external sites may use different assemblies or annotations than FlyBase.

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