FlyBase Gene Report: Dmel\Aplip1

General Information

Symbol

Dmel\Aplip1

Species

D. melanogaster

Name

APPL-interacting protein 1

Annotation Symbol

CG1200

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040281

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

APPL-interacting protein 1 (Aplip1) encodes a scaffolding protein that can bind to several proteins such as Klc, Appl, and hep. Aplip1 can link Khc to vesicles and has roles in fast axonal transport that are important for neuron structure and function. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

E(Khc)ek4, SP512, Aplip, dp1

Key Links

Genomic Location

Cytogenetic map

61F3-61F3

Sequence location

3L:1,196,317..1,199,339 [+]

Recombination map

3-0

RefSeq locus

NT_037436 REGION:1196317..1199339

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables kinesin binding

inferred from physical interaction with FLYBASE:Klc; FB:FBgn0010235

(Horiuchi et al., 2005, Taru et al., 2002)

enables MAP-kinase scaffold activity

inferred from physical interaction with UniProtKB:Q23977

(Taru et al., 2002)

enables protein binding

inferred from physical interaction with FLYBASE:Appl; FB:FBgn0000108

(Taru et al., 2002)

enables protein kinase binding

inferred from physical interaction with FLYBASE:hep; FB:FBgn0010303

(Taru et al., 2002)

NOT enables JUN kinase binding

inferred from physical interaction with FLYBASE:bsk; FB:FBgn0000229

(Taru et al., 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

NOT enables JUN kinase binding

inferred from sequence alignment with MGI:MGI:1309464

(Taru et al., 2002)

inferred from sequence alignment with MGI:MGI:1926555

(Taru et al., 2002)

Biological Process (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in anterograde synaptic vesicle transport

inferred from mutant phenotype

(Horiuchi et al., 2005)

involved_in axonal transport of mitochondrion

inferred from mutant phenotype

(Horiuchi et al., 2005)

involved_in regulation of JNK cascade

inferred from physical interaction with FLYBASE:hep; FB:FBgn0010303

(Taru et al., 2002)

involved_in regulation of terminal button organization

inferred from mutant phenotype

(Klinedinst et al., 2013)

involved_in retrograde synaptic vesicle transport

inferred from mutant phenotype

(Horiuchi et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in JNK cascade

inferred from sequence alignment with MGI:MGI:1309464

(Taru et al., 2002)

inferred from sequence alignment with MGI:MGI:1926555

(Taru et al., 2002)

involved_in regulation of JNK cascade

inferred from electronic annotation with InterPro:IPR047178

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q9WVI9

(Gene Ontology Curators, 2002-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Horiuchi et al., 2005)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the JIP scaffold family. (Q9W0K0)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity).

(UniProt, Q9W0K0)

Summary (Interactive Fly)

JNK scaffolding scaffolding protein that is part of motor-cargo linkage complexes for both kinesin-1 and dynein - acts downstream of the JNK pathway to affect axonal transport - binds APP family proteins

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Aplip1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W0K0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

4Z88

Comments on Gene Model

Gene model reviewed during 5.48

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Aplip1-RA

FBtr0072626

NM_167857

2390

483

Aplip1-RB

FBtr0072627

NM_167856

2411

490

Additional Transcript Data and Comments

Reported size (kB)

2.4 (northern blot)

(Taru et al., 2002)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Aplip1-PA

FBpp0072522

53.1

483

5.77

NP_728574

AAF47446

Aplip1-PB

FBpp0072523

53.8

490

5.86

Q9W0K0

NP_728573

AAN11462

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

490 (aa)

(Taru et al., 2002)

Comments

External Data

Subunit Structure (UniProtKB)

Forms homo- and heterooligomeric complexes. Binds Hep, a dual specificity protein kinase in the JNK pathway, but not its downstream target bsk. The C-terminal region interacts with the kinesin light chain protein, Klc, and the C-terminal PTY motif of amyloid-beta protein precursor-like protein, Appl.

(UniProt, Q9W0K0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

4Z88

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W0K0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Aplip1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Aplip1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.79

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 7 -- 8

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa primordium

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

late embryonic stage

embryonic/larval brain

(Taru et al., 2002)

embryonic/larval central nervous system

(Taru et al., 2002)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

organism

(Taru et al., 2002)

larval stage -- adult stage

(Taru et al., 2002)

adult stage

head

(Taru et al., 2002)

Additional Descriptive Data

Aplip1 transcripts are abundant in late embryonic through adult stages and are weakly expressed in 6-12 hour embryos as observed on northern blots. Transcripts are observed in adult heads but not bodies by RT-PCR.

(Taru et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Horiuchi et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Aplip1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Aplip1

View exonic expression by tissue for Dmel\Aplip1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040281

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53472/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040281

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040281

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040281

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1200

Flygut - An atlas of the Drosophila adult midgut

FBgn0040281

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Aplip1

Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Aplip1

Transgenic constructs containing regulatory region of Aplip1

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(3L)Ar14-8

(Horiuchi et al., 2005)

Df(3L)bab-PG

(Horiuchi et al., 2005)

Gene not disrupted in

Df(3L)Fpa1

(Horiuchi et al., 2005)

Df(3L)Fpa2

(Horiuchi et al., 2005)

Df(3L)ru-22

(Horiuchi et al., 2005)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | larval stage, with Scer\GAL4^Toll-6-D42

Aplip1^UAS.Tag:FLAG

lethal | pupal stage, with Scer\GAL4^Toll-6-D42

Aplip1^UAS.Tag:FLAG

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Aplip1^GD7456

viable

viable, with Scer\GAL4^pnr-MD237

Aplip1^GD7456

viable, with Scer\GAL4^tin.CΔ4

Aplip1^GD7456

viable (with Df(3L)Fpa2)

Aplip1^ex

Sterility

Allele

fertile

Aplip1^EK4

Other Phenotypes

Allele

abnormal neuroanatomy

Aplip1^EK4

abnormal neuroanatomy, with Scer\GAL4^Toll-6-D42

Aplip1^UAS.Tag:FLAG

abnormal neuroanatomy (with Df(3L)Fpa2)

Aplip1^EK4

abnormal neuroanatomy | larval stage, with Scer\GAL4^BG380

Aplip1^UAS.cKa

abnormal neuroanatomy | larval stage (with Df(3L)Fpa2)

Aplip1^ex

abnormal neuroanatomy | second instar larval stage

Aplip1^ex

abnormal neuroanatomy | third instar larval stage

Aplip1^ex

paralytic

Aplip1^EK4

paralytic | larval stage | posterior

Aplip1^EK4

paralytic | larval stage | posterior, with Scer\GAL4^Toll-6-D42

Aplip1^UAS.Tag:FLAG

paralytic | larval stage | posterior (with Df(3L)Fpa2)

Aplip1^EK4

Phenotype manifest in

Allele

axon

Aplip1^EK4

axon, with Scer\GAL4^Toll-6-D42

axon (with Df(3L)Fpa2)

Aplip1^EK4

mitochondrion

Aplip1^EK4

NMJ bouton | larval stage, with Scer\GAL4^BG380

Aplip1^UAS.cKa

NMJ bouton | larval stage (with Df(3L)Fpa2)

Aplip1^ex

NMJ bouton | second instar larval stage

Aplip1^ex

NMJ bouton | third instar larval stage

Aplip1^ex

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (30)

Hsap\MAPK8IP1

9 of 14

Yes

Hsap\MAPK8IP2

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\APBB2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC112267931

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Mapk8ip1

8 of 14

Yes

Rnor\Mapk8ip2

7 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (17)

Mmus\Mapk8ip1

9 of 14

Yes

Mmus\Mapk8ip2

8 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\mapk8ip1

6 of 13

Yes

Xtro\mapk8ip2

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\entpd2

1 of 13

Xtro\f2r

1 of 13

Yes

Xtro\kif1a

1 of 13

Xtro\ldlrap1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (22)

Drer\mapk8ip1a

8 of 14

Yes

Drer\mapk8ip2

8 of 14

Yes

Drer\mapk8ip1b

3 of 14

Yes

Drer\fam43a

2 of 14

Drer\fam43b

2 of 14

Drer\ldlrap1a

2 of 14

Yes

Drer\ldlrap1b

2 of 14

Yes

Drer\si:dkey-71h2.2

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:ch211-204c21.1

1 of 14

Drer\si:dkey-34e4.1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\jip-1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\lin-10

1 of 14

Cele\nck-1

1 of 14

Cele\num-1

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP006021

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\NBP2

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Aplip1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

ced-6

3 of 13

CG8312

3 of 13

CG42673

3 of 13

Dab

3 of 13

numb

3 of 13

CG14968

2 of 13

Exn

2 of 13

Abi

1 of 13

CAP

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Shc

1 of 13

X11Lβ

1 of 13

X11L

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MAPK8IP1; mitogen-activated protein kinase 8 interacting protein 1

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Aplip1^KK107996

ameliorates Parkinson's disease 8

modeled by Lrrk¹

(Inoshita et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAPK8IP1; mitogen-activated protein kinase 8 interacting protein 1

9 of 14

604641

TYPE 2 DIABETES MELLITUS; T2D

type 2 diabetes mellitus

MAPK8IP2; mitogen-activated protein kinase 8 interacting protein 2

8 of 14

607755

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Aplip1

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Horiuchi et al., 2005, Taru et al., 2002)

Aplip1 - AP-1μ

experimental knowledge based

(Guruharsha et al., 2011)

Aplip1 - Appl

pull down, western blot, anti tag coimmunoprecipitation, two hybrid, anti bait coimmunoprecipitation, anti tag western blot

(Taru et al., 2002)

Aplip1 - Klc

anti tag coimmunoprecipitation, anti tag western blot

(Horiuchi et al., 2007, Horiuchi et al., 2005, Taru et al., 2002)

Aplip1 - Rbp

two hybrid, isothermal titration calorimetry, predetermined participant, x-ray crystallography

(Petzoldt et al., 2020, Siebert et al., 2015)

Aplip1 - hep

pull down, anti tag western blot, anti tag coimmunoprecipitation

(Horiuchi et al., 2007, Taru et al., 2002)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Aplip1

suppressible

Dhc64C

(Horiuchi et al., 2005)

Aplip1

suppressible

futsch

(Klinedinst et al., 2013)

Aplip1

suppressible

hiw

(Klinedinst et al., 2013)

Aplip1

suppressible

Klp64D

(Horiuchi et al., 2005)

Aplip1

suppressible

wnd

(Klinedinst et al., 2013)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

hiw

suppressible

Aplip1

(Klinedinst et al., 2013)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9W0K0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

72744

DroID - A comprehensive database of gene and protein interactions.

FBgn0040281

MIST (genetic) - An integrated Molecular Interaction Database

53472

MIST (protein-protein) - An integrated Molecular Interaction Database

53472

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9W0K0

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-0

Cytogenetic map

61F3-61F3

Sequence location

3L:1,196,317..1,199,339 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

61F3-61F3

Limits computationally determined from genome sequence between P{PZ}l(3)05967⁰⁵⁹⁶⁷ and P{PZ}l(3)02640⁰²⁶⁴⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

61F1-61F2

(Serano, 2000.2.4)

61F-61F

(Yagi, 1999.3.8)

Located on the left arm of chromosome 3.

(Gassman et al., 1997)

Experimentally Determined Recombination Data

Location

3-0

(FlyBase, 2016)

3-1.3

(Comeron et al., 2012)

3-0.0

(Gassman, 1999.3.11)

(Gassman et al., 1997)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24632

Aplip1^EK4

24634

w^*; P{UASp-Aplip1.EGFP}3

26024

y¹ v¹; P{TRiP.JF02049}attP2

79425

y¹ sc^* v¹ sev²¹; P{TOE.GS01172}attP40

95040

y¹ w^*; TI{CRIMIC.TG4.2}Aplip1^{CR70337-TG4.2}/TM3, Sb¹, Ser¹

FlyORF

F002870

M{UAS-Aplip1.ORF.3xHA.GW}ZH-86Fb

VDRC

v109501

P{KK107996}VIE-260B

v50007

w¹¹¹⁸; P{GD7456}v50007

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (52)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH14842

Other clones

DPiM_GH14842

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040281

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040281

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: as a protein that interacts with the Appl cytoplasmic domain in a yeast two-hybrid assay.

(Taru et al., 2002)

Identification: Protein that binds the cytoplasmic domain of Appl in a yeast two-hybrid screen.

(Yagi et al., 1999)

Identification: Dominant enhancer of Khc mutations.

(Gassman et al., 1997)

Mutations when homozygous or combined with either Khc or Klc causes organelle jams (axonal swellings filled with membrane-bounded organelles that are stalled in transit) and flipping (posterior muscle activity causing a rhythmic upward flipping of the tail during crawling). No effects are seen in combination with Dhc64C or Gl.

(Gassman et al., 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: SP512 CG1200

(Bayraktaroglu, 2000.8.7)

Source for merge of: Aplip1 dp1

(Suzuki, 2002.9.13)

Source for merge of: Aplip1 E(Khc)ek4

(Horiuchi et al., 2005)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Aplip1 SP512

(Huen, 2002.5.16)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (22)

Reported As

Symbol Synonym

APLIP1

(Bouleau and Tricoire, 2015, Horiuchi et al., 2005, Katz et al., 2003, Huen, 2002.5.16, Taru et al., 2002)

Aplip

(Turrel et al., 2022, Jiang et al., 2019)

Aplip-1

(Böhme et al., 2019)

CG1200

(Neuert et al., 2017, Llamusi et al., 2013, Howell et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Sepp et al., 2008, Katz et al., 2003, Huen, 2002.5.16)

E(Khc)ek4

(Gassman, 1999.3.11, Martin et al., 1999)

EK4

(Gassman et al., 1999)

Ek4

(Gassman et al., 1997)

JIP-1

(Katz et al., 2003)

JIP1

(Horiuchi et al., 2007)

Jip1

(Böhme et al., 2019)

Jip1/2

(Horiuchi et al., 2005)

SP512

(Katz et al., 2003, Huen, 2002.5.16)

aplilp1

(Bornstein et al., 2015)

aplip1

(Michaut et al., 2003)

dp1

(Yagi, 1999.3.8)

ek4

(Gassman et al., 2001)

jip1

(Klinedinst et al., 2013)

Name Synonyms

APP-like protein interacting protein 1

(Taru et al., 2002)

APPL-interacting protein 1

(Taru et al., 2002)

JNK interacting protein-1

(Klinedinst et al., 2013)

c-Jun N-terminal kinase (JNK)-interacting protein

(Horiuchi et al., 2007)

Secondary FlyBase IDs

FBgn0020447
FBgn0026251
FBgn0035188

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53472

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W0K0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W0K0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W0K0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0040281

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53472/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040281

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040281

FlyMine - An integrated database for Drosophila genomics

FBgn0040281

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1200

MARRVEL_MODEL - MARRVEL (model organism gene)

53472

PDB - An information portal to biological macromolecular structures

4Z88

Linkouts

BioGRID - A database of protein and genetic interactions.

72744

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040281

DroID - A comprehensive database of gene and protein interactions.

FBgn0040281

DRSC - Results frm RNAi screens

FBgn0040281

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Aplip1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040281

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040281

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1200

Flygut - An atlas of the Drosophila adult midgut

FBgn0040281

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002493

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

53472

MIST (protein-protein) - An integrated Molecular Interaction Database

53472

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9W0K0

References (74)

The following external sites may use different assemblies or annotations than FlyBase.

-0.79

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