dDis3, Tazman
Please see the JBrowse view of Dmel\Dis3 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dis3 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: 0-12 hr AEL
Dis3 transcript can be detected by Northern analysis from 0-12 hrs AEL, but not from 12-20 hrs AEL; expression peaks at 6-8 hrs AEL. Dis3 transcript is also detected during larval, pupal, and adult stages, with a second peak during the first larval instar. Dis3 transcript is detected by in situ hybridization starting at oogenesis stage 3, and is abundant in nurse cells. Maternally deposited transcript is abundant in embryos through cellularization. Dis3 transcript is ubiquituously expressed during gastrulation, and particularly abundant in mesoderm. Expression is less instance by germ band retraction.
Dis3 protein can be detected by Western analysis from 0 hrs AEL through the third larval instar; expression peaks at 6-8 hr AEL, drops sharply at 12-24 hr AEL, and is barely detectable at larval stages. Expression is also detected in female but not male adults. Dis3 protein distribution in embyros is similar to the distribution of Dis3 transcipt; ubiquitous immunoreactivity is observed through germ band retraction, and is more pronounced in mesoderm.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Dis3 in JBrowse3-84
3-83.0
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
New stable cell line derived from S2-unspecified : Stable S2 cell lines were created expressing copper-inducible epitope-tagged exosome subunits to examine the subcellular distribution of exosome complex components. Stable lines were created containing the following exosome subunits; Dis3, Rrp6, Mtr3, Rrp42, Rrp4, Ski6, Rrp46, Rrp40, and Csl4.
Identification: as a protein that copurifies with Spt6 protein.
Source for identity of Dis3 CG6413 was sequence comparison ( date:010401 ).
Source for identity of: Dis3 CG6413
