20xUAS drives expression of CsChrimson fused to Venus. Golgi and ER export motifs have also been added to the coding sequence. FlyBase curator comment: the original published version of FBrf0224260 erroneously stated that this construct contained Chrimson rather than CsChrimson; this has since been corrected in the online published version (for details see also the corrigendum FBrf0226484 and the addendum FBrf0227011).
In larvae expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in motor neurons (via Scer\GAL4VGlut-OK371), both 470-nm and 617-nm light triggers EJPs, even at short (1- to 2-ms) light-pulse durations and low intensities. In response to light-pulse durations over 2 ms, Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus expression triggers long-lasting barrages of EJPs. Long-wavelength red light (720 nm) also triggers EJPs, though high-intensity light and long light-pulse durations (40–160 ms) are required for robust activation of the NMJ.
In adults expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in sweet taste receptor cells (via Scer\GAL4Gr64f.9.7), proboscis extension reflexes are robustly elicited at very low 470 nm and 617 nm light intensities. Flies also respond to 720-nm light at an intensity substantially lower than that required in the larvae.
Adults expressing Zzzz\ChR1IVS.20xScer\UAS.T:Avic\GFP-YFP.Venus in PNv-1 antennal lobe neurons that innervate the V glomerulus (via Scer\GAL4VT31497) which induces an avoidance response to CO[[2]], reliably avoid quadrants in a circular arena lit by weak red light (617 nm).
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by rut1
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by dnc1
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAVP16.Mef2.247
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4, norpAP24 has abnormal locomotor behavior | adult stage | conditional phenotype, non-enhanceable by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAGMR38G08
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12 has abnormal locomotor behavior | larval stage | conditional phenotype, non-enhanceable by Ecol\lexAGMR27H06/Hsap\KCNJ2lexAop.cHa
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12 has abnormal locomotor behavior | larval stage | conditional phenotype, non-enhanceable by Ecol\lexAp65.Ilp7.DP/Hsap\KCNJ2lexAop.cHa
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4EL.eveRC has abnormal locomotor behavior | larval stage phenotype, suppressible by Df(2R)eve/Df(2R)eve/eveΔEL/eveΔEL
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by norpAP24
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Dop1R1f02676
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Dop2Rf06521
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by pleHMC06137, Scer\GAL40273-G4
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAGMR13F02
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4, norpAP24 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAGMR13F02
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4, norpAP24 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAVP16.Mef2.247
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | conditional | partially by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAGMR38G08
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Hsap\RELAAD.R23E07, Scer\GAL4DBD.VT002081 has abnormal locomotor behavior | larval stage | conditional phenotype, suppressible by VAChTGD3054, Hsap\RELAAD.R23E07, Scer\GAL4DBD.VT002081
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR23E10 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by Inx6GD3692, Scer\GAL4GMR23E10
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR23E10 has abnormal neurophysiology | adult stage | conditional phenotype, suppressible | partially by Inx6GD3692, Scer\GAL4GMR23E10
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR23E10 has abnormal sleep | adult stage | conditional phenotype, suppressible | partially by Inx6GD3692, Scer\GAL4GMR23E10
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4NPF.1 has abnormal locomotor behavior | adult stage | conditional phenotype, suppressible | partially by NPFRNAi.UAS, Scer\GAL4NPF.1
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by rut1
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by dnc1
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAVP16.Mef2.247
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL40273-G4, norpAP24 has abnormal locomotor behavior | adult stage | conditional phenotype, non-suppressible by Gthe\ACR1lexAop.d.EYFP/Ecol\lexAGMR38G08
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12 has abnormal locomotor behavior | larval stage | conditional phenotype, non-suppressible by Ecol\lexAGMR27H06/Hsap\KCNJ2lexAop.cHa
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12 has abnormal locomotor behavior | larval stage | conditional phenotype, non-suppressible by Ecol\lexAp65.Ilp7.DP/Hsap\KCNJ2lexAop.cHa
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus/Scer\GAL4Tdc2.PC is a suppressor | conditional of abnormal song | conditional phenotype of Crei\ChR1::Vcar\ChR1::Vcar\ChR2ReaChR.lexAop.FRT.mCherry.Citrine, Ecol\lexAVT040556.PU, Scer\FLP1fru
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR23E10 is a suppressor | conditional | partially of abnormal sleep | adult stage phenotype of Inx6GD3692, Scer\GAL4GMR23E10
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR23E10 has fan-shaped body | conditional phenotype, suppressible | partially by Inx6GD3692, Scer\GAL4GMR23E10
Scer\GAL4Gr66a.1.8/Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus is a suppressor of labellum | conditional phenotype of Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAp65.nompC