FlyBase Allele Report: Scer\GAL4[Ilp7.PY]

General Information

Symbol

Scer\GAL4^Ilp7.PY

Species

S. cerevisiae

Name

Insulin-like peptide 7 promoter construct of Yang

FlyBase ID

FBal0219112

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ilp7-GAL4.Y}

Also Known As

ILP7-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Yang et al., 2008)

Progenitor genotype

Carried in construct

P{Ilp7-GAL4.Y}

Cytology

Description

Ilp7 promoter sequences regulate expression of a GAL4 driver.

(Yang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

Ilp7

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval Ilp7 neuron

(Hu et al., 2017)

adult stage

subesophageal zone neuron | subset

(Yang et al., 2008)

neuron of adult ventral nerve cord | restricted

(Yang et al., 2008)

female reproductive system | restricted

(Yang et al., 2008)

Additional Information

Statement

Reference

Marker for

embryonic/larval Ilp7 neuron

(Hu et al., 2017)

Reflects expression of

Ilp7

(Hu et al., 2017, Yang et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 625 nm (red) light) of Scer\GAL4^Ilp7.PY cells in larvae significantly increases nociceptive movement responses following stimulation with a von Frey filament, but has no effect in the absence of pain stimuli, compared to controls.

Inhibition (using Hsap\KCNJ2^UAS.EGFP, but not Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Ilp7.PY cells in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; in the case of Hsap\KCNJ2^UAS.EGFP there is no effect on the response to gentle touch or locomotion speed, compared to controls; neither of these manipulations affect the latency of rolling in response to high temperature (local) stimulation, compared to controls.

(Hu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\KCNJ2^UAS.EGFP/Scer\GAL4^Ilp7.PY is a suppressor | partially of abnormal locomotor behavior | larval stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR27H06

(Hu et al., 2017)

Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Ilp7.PY, Scer\GAL4^GMR82E12 is a suppressor | partially of abnormal locomotor behavior | larval stage | conditional phenotype of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^GMR27H06