A 2571 base pair fragment from the flanking non-coding or intronic region of Dscam is fused upstream of a Drosophila core synthetic promoter (DSCP) followed by sequence encoding a lexA::p65 driver. The driver sequence has been optimized for Drosophila codon usage. An Hsp70 transcriptional terminator is present.
Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus with 625 nm (red) light) of Ecol\lexAGMR27H06 cells in larvae significantly increases rolling responses in the absence of pain stimuli, compared to controls.
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR27H06 has abnormal locomotor behavior | larval stage | conditional phenotype, suppressible | partially by Hsap\KCNJ2UAS.EGFP/Scer\GAL4Rab11.6.14.3
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR27H06 has abnormal locomotor behavior | larval stage | conditional phenotype, suppressible | partially by Hsap\KCNJ2UAS.EGFP/Scer\GAL4GMR82E12
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR27H06 has abnormal locomotor behavior | larval stage | conditional phenotype, suppressible | partially by Hsap\KCNJ2UAS.EGFP/Scer\GAL4Ilp7.PY
Cnoc\ChR1::Csub\ChRCsChrimson.IVS.13XLexAop2.Venus, Ecol\lexAGMR27H06 has abnormal locomotor behavior | larval stage | conditional phenotype, suppressible | partially by Hsap\KCNJ2UAS.EGFP/Scer\GAL4Ilp7.PY/Scer\GAL4GMR82E12
Ecol\lexAGMR27H06/Hsap\KCNJ2lexAop.cHa is a non-enhancer of abnormal locomotor behavior | larval stage | conditional phenotype of Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12
Ecol\lexAGMR27H06/Hsap\KCNJ2lexAop.cHa is a non-suppressor of abnormal locomotor behavior | larval stage | conditional phenotype of Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus, Scer\GAL4GMR82E12
Although alleles from the Janelia Farm lexA driver collection incorporate the same enhancer fragments used to create the Janelia Farm GAL4 driver alleles (GMR_Brain_exp_1), significant differences are frequently observed in the expression pattern of a given enhancer fragment inserted in the P{CaryP}attP40 or other docking site compared to the same fragment inserted in the P{CaryP}attP2 docking site. Generally the Ecol\lexA patterns are subsets of the Scer\GAL4 patterns.