FlyBase Allele Report: Scer\GAL4[DBD.R72B05]

General Information

Symbol

Scer\GAL4^DBD.R72B05

Species

S. cerevisiae

Name

FlyBase ID

FBal0304478

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R72B05-GAL4.DBD}

Also Known As

R72B05

Transgenic product class

Mutagen

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct - coding region fusion

(Aso et al., 2014)

in vitro construct - regulatory fusion

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R72B05-GAL4.DBD}

Cytology

Description

The R72B05 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR72B05

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval mushroom body dopaminergic neuron f1

Comment: when combined with P{R15B01-p65.AD} (combination referred to as 'MB145')

when combined with P{R15B01-p65.AD} (combination referred to as 'MB145B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Truman et al., 2023, Eschbach et al., 2020, Eichler et al., 2017)

larval mushroom body dopaminergic neuron c1

Comment: when combined with P{R15B01-p65.AD} (combination referred to as 'MB145')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Truman et al., 2023, Eschbach et al., 2020)

adult stage

MB-alpha neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')

when combined with PBac{ple-p65.AD} (combination referred to as 'MB065B')

when combined with P{VT026773-p65.AD} (combination referred to as 'MB630B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Feng et al., 2021, Aso and Rubin, 2016, Aso et al., 2014)

MB-alpha' neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')

when combined with PBac{ple-p65.AD} (combination referred to as 'MB065B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Aso and Rubin, 2016)

mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')

when combined with PBac{ple-p65.AD} (combination referred to as 'MB065B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)

mushroom body vertical lobe arborizing neuron 1

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')

when combined with PBac{ple-p65.AD} (combination referred to as 'MB065B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)

mushroom body alpha lobe slice 3

Comment: when combined with P{VT026773-p65.AD} (combination referred to as 'MB630B')

(Aso and Rubin, 2016)

MB-alpha' neuron of the dopaminergic PPL1 cluster | faint

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')

when combined with P{ple-p65.AD} (combination referred to as 'MB065B')

(Aso et al., 2014)

mushroom body medial-vertical lobe arborizing neuron 1 | intense

Comment: when combined with P{R82C10-p65.AD} (combination referred to as 'MB060B')). 190219.

(Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.ple

(Takemura et al., 2017)

abnormal behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.VT026773

(Takemura et al., 2017)

abnormal memory | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.VT026773

(Takemura et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72B05 and Hsap\RELA^AD.VT026773 in adult females, in combination with a paired odor, results in a long-term aversive olfactory memory for this odor; avoidance is significantly greater when these neurons are not also activated during the testing period.

Adult females prefer optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72B05 and Hsap\RELA^AD.VT026773, or the split-GAL4 pair of Scer\GAL4^DBD.R72B05 and Hsap\RELA^AD.ple, to darkness with no activation; this preference is significantly greater than that of controls for red light compared to darkness.

(Takemura et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions