FB2024_03 , released June 25, 2024
Recombinant Construct: PBac{ple-p65.AD}
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General Information
Symbol
PBac{ple-p65.AD}
FlyBase ID
FBtp0117250
Feature type
transgenic_transposable_element
Component Allele(s)
Hsap\RELAAD.ple
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele Hsap\RELAAD.ple
Transgenic product class
Component
Use(s)
Encoded product / tool
p65(AD)::Zip+
Description

The ple promoter region is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
larval mushroom body dopaminergic neuron c1

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

when combined with P{R72G06-GAL4.DBD} (combination referred to as 'MB586B')

(Truman et al., 2023, Eschbach et al., 2020)
larval mushroom body dopaminergic neuron f1

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

(Truman et al., 2023, Eschbach et al., 2020)
adult stage
MB-alpha neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
MB-alpha' neuron of the dopaminergic PPL1 cluster | faint

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

(Aso et al., 2014)
mushroom body medial-vertical lobe arborizing neuron 1

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

when combined with P{R73F07-GAL4.DBD} (combination referred to as 'MB099C')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
mushroom body vertical lobe arborizing neuron 1

Comment: when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

when combined with P{R73F07-GAL4.DBD} (combination referred to as 'MB099C')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
MB-alpha neuron of the dopaminergic PPL1 cluster | faint

Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

(Aso et al., 2014)
MB-alpha' neuron of the dopaminergic PPL1 cluster

Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

when combined with P{R72B05-GAL4.DBD} (combination referred to as 'MB065B')

(Zhang et al., 2022, Aso and Rubin, 2016, Aso et al., 2014)
MB-alpha' neuron of the dopaminergic PPL1 cluster | intense

Comment: when combined with P{R26F01-GAL4.DBD} (combination referred to as 'MB308B')

(Aso et al., 2014)
mushroom body pedunculus-medial lobe arborizing neuron 1

Comment: when combined with P{R22B12-GAL4.DBD} (combination referred to as 'MB320C')

(Zhang et al., 2022, Feng et al., 2021, Aso and Rubin, 2016)
dopaminergic PPL2ab neuron

Comment: when combined with P{R42H01-GAL4.DBD} (combination referred to as 'LH2454')

(Dolan et al., 2019)
EB neuron of the dopaminergic PPM3 cluster

Comment: when combined with P{Ddc-GAL4.DBD}

(Gao et al., 2019)
adult dopaminergic VUM neuron of the gnathal ganglion

Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

(Sterne et al., 2021)
adult ascending neuron | subset

Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

(Sterne et al., 2021)
dopaminergic PAM neuron | subset

Comment: when combined with P{VT064569-GAL4.DBD} (combination referred to as 'SS46889')

(Sterne et al., 2021)
dopaminergic PPL1 fan-shaped body layer 5 neuron

Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

(Hulse et al., 2021)
dopaminergic PPL1 fan-shaped body layer 6 neuron

Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

(Hulse et al., 2021)
dopaminergic PPL1 fan-shaped body layer 7 neuron

Comment: when combined with P{VT025720-GAL4.DBD} (combination referred to as 'SS56699')

(Hulse et al., 2021)
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
ple
(Aso and Rubin, 2016)
Reporter construct used in assay
Images
Virtual Fly Brain - A database to explore neuroanatomy, connectivity and gene expression
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (4)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
PBac{ple-p65.AD}
(Rubin, 2017.6.14, FlyBase, 1992-)
TH-p65ADZp
(Jia et al., 2021)
Secondary FlyBase IDs
    References (21)