FB2024_03 , released June 25, 2024
Allele: Scer\GAL4Gr66a.1.8
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General Information
Symbol
Scer\GAL4Gr66a.1.8
Species
S. cerevisiae
Name
FlyBase ID
FBal0249510
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Gr66a-GAL4.1.8}
Also Known As
Gr66a-GAL4, Gr66a
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Weiss et al., 2011)
Progenitor genotype
Carried in construct
P{Gr66a-GAL4.1.8}
Cytology
Description

A 1.8kb Gr66a promoter fragment is fused upstream of sequence encoding a GAL4 driver.

(Weiss et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
Gr66a
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      gustatory receptor neuron of terminal organ
      (Kwon et al., 2011)
      gustatory receptor neuron of terminal organ dorsolateral group
      (Kwon et al., 2011)
      gustatory receptor neuron of the epiphysis
      (Kwon et al., 2011)
      larval hypopharyngeal organ gustatory receptor neuron
      (Kwon et al., 2011)
      gustatory receptor neuron of the hypophysis
      (Kwon et al., 2011)
      larval gnathal ganglion
      (Kwon et al., 2011)
      larval gustatory receptor neuron of the epiphysis DP1
      (Choi et al., 2016)
      larval gustatory receptor neuron of the epiphysis DP2
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypophysis VP1
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypophysis VP2
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP1
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP2
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP3
      (Choi et al., 2016)
      larval gustatory receptor neuron of the hypopharyngeal organ PP4
      (Choi et al., 2016)
      adult stage
      high salt/bitter-sensing neuron | subset of prothoracic tarsal segment 5
      (Ling et al., 2014)
      gustatory receptor neuron | subset of short labellar taste bristle | subset
      (Weiss et al., 2011)
      gustatory receptor neuron | subset of intermediate labellar taste bristle | subset
      (Weiss et al., 2011)
      high salt/bitter-sensing neuron of adult labellum sensillum
      (Weiss et al., 2011)
      bitter-sensitive labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a
      (Weiss et al., 2011)
      intermediate labellar taste bristle
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b
      (Weiss et al., 2011)
      short labellar taste bristle S-b
      (Weiss et al., 2011)
      short labellar taste bristle S-a | intense
      (Weiss et al., 2011)
      bitter-sensitive labellar taste bristle | intense
      (Weiss et al., 2011)
      short labellar taste bristle S-b | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-a | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle | intense
      (Weiss et al., 2011)
      intermediate labellar taste bristle I-b | intense
      (Weiss et al., 2011)
      adult abdominal multidendritic neuron | subset
      (Park and Kwon, 2011)
      short labellar taste bristle S-a
      (Weiss et al., 2011)
      bitter-sensing neuron | subset
      (Israel et al., 2022)
      Additional Information
      Statement
      Reference

      Drives expression in bitter-sensing neurons.

      (FlyBase Curators, 2017)

      ScerGAL4Gr66a.1.8 drives expression in the following series of neurons. Larval gustatory receptor neuron of the epiphysis DP1, where it is coexpressed with ScerGAL4Gr33a.4.7, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr93a.5, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr9a.6.3, ScerGAL4Gr22d.3.1, ScerGAL4Gr22e.2.5, ScerGAL4Gr28b.a.3, ScerGAL4Gr39a.b.1, ScerGAL4Gr58b.1, and ScerGAL4Gr59d.4.1. Larval gustatory receptor neuron of the epiphysis DP2 where it is coexpressed with ScerGAL4Gr33a.4.7, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr77a.4 and ScerGAL4Gr93b.5.3. Larval gustatory receptor neuron of the hypophysis VP1 and VP2 where it is coexpressed with ScerGAL4Gr33a.4.7 and ScerGAL4Gr68a.PB. Larval gustatory receptor neuron of the hypopharyngeal organ PP1 and PP2 where it is coexpressed with ScerGAL4Gr33a.4.7, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4 Larval gustatory receptor neuron of the hypopharyngeal organ PP3 where it is coexpressed with ScerGAL4Gr33a.4.7, ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr28a.PT, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4. Larval gustatory receptor neuron of the hypopharyngeal organ PP4 where it is coexpressed with ScerGAL4Gr33a.4.7, ScerGAL4Gr28a.PT, ScerGAL4Gr22b.5.8, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39b.4.4, ScerGAL4Gr39a.d.1.7, ScerGAL4Gr93c.3.8 and ScerGAL4Gr93d.2.4.

      (Choi et al., 2016)

      ScerGAL4Gr66a.1.8 drives expression in adult abdominal multidendritic neurons with with dorsal processes extending along the left-right axis, as well as in multidendritic neurons with lateral processes extending anteroposteriorly; these major processes have several protruding dendrites. Additionally, expression is driven in laterally localized multidendritic neurons with extensive arborization. Expression is also observed in projections to the abdominal ganglion.

      (Park and Kwon, 2011)

      ScerGAL4Gr66a.1.8 is expressed in gustatory neurons of the bitter-sensitive short labellar taste bristles, and in gustatory neurons of all of the intermediate labellar taste bristles.

      (Weiss et al., 2011)

      ScerGAL4Gr66a.1.8 is strongly expressed in almost all of the short and intermediate taste sensilla of the labellum.

      (Weiss et al., 2011, Weiss et al., 2011)

      ScerGAL4Gr66a.1.8 drives expression in 6 gustatory receptor neurons of the larval terminal organ, two of these having its cell body in the dorsal organ ganglion (TO-dorsolateral group). All 6 neurons co-express ScerGAL4Gr33a.4.7. One of the neurons of the TO-dorsolateral group also expresses ScerGAL4Gr10a.3.7 and ScerGAL4Gr32a.3.7.PW. The other terminal organ neurons express different combinations of drivers. The first neuron expresses ScerGAL4Gr32a.3.7.PW, ScerGAL4Gr28b.e.4.245, ScerGAL4Gr39a.a.3.4, ScerGAL4Gr39a.b.1, ScerGAL4Gr59d.4.1, ScerGAL4Gr9a.6.3, ScerGAL4Gr22e.2.5, ScerGAL4Gr22a.4.2, ScerGAL4Gr28b.a.3, ScerGAL4Gr36b.0.9, ScerGAL4Gr36c.2.5, ScerGAL4Gr58b.1, ScerGAL4Gr59a.1.5, ScerGAL4Gr59c.5.5 and ScerGAL4Gr93b.5.3. The second neuron co-expresses ScerGAL4Gr39a.a.3.4, ScerGAL4Gr57a.6, ScerGAL4Gr59d.4.1, ScerGAL4Gr47b.2 and ScerGAL4Gr94a.3.7. The third neuron co-expresses ScerGAL4Gr57a.6 and ScerGAL4Gr97a.1. The fourth neuron expresses ScerGAL4Gr39a.b.1 and ScerGAL4Gr59d.4.1. Expression of ScerGAL4Gr66a.1.8 is also found in two neurons of the dorsal pharyngeal sense organ (DPS or epiphysis), two neurons of the posterior pharyngeal sense organ (PPS or hypopharyngeal sense organ) and in two neurons of the ventral pharyngeal sense organ (VPS or hypophysis).

      (Kwon et al., 2011)
       
      Marker for
      bitter-sensing neuron
      (Israel et al., 2022)
      Reflects expression of
      Gr66a
      (Israel et al., 2022, Ling et al., 2014, Kwon et al., 2011, Park and Kwon, 2011, Weiss et al., 2011, Weiss et al., 2011)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of Scer\GAL4Gr66a.1.8 cells in adult females does not affect preference for softer 100 mM sucrose-containing agarose for egg laying, compared to controls.

      (Wu et al., 2019)

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 617 nm (red) light) of Scer\GAL4Gr66a.1.8 cells, or the combination of Scer\GAL4Gr66a.1.8 and Scer\GAL4nompC.PC cells, in adults results in induction of proboscis retraction due to stimulation during feeding, compared to controls.

      (Zhou et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4Gr66a.1.8
      Name Synonyms
      Secondary FlyBase IDs
        References (28)