FlyBase Allele Report: Dmel\foi[C887]

General Information

Symbol

Dmel\foi^C887

Species

D. melanogaster

Name

FlyBase ID

FBal0319773

Feature type

allele

Associated gene

Dmel\foi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C2503T.

(Carrasco-Rando et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,532,800..8,532,800 [+]

Nucleotide change:

C8532800T

Reported nucleotide change:

C2614T

Amino acid change:

Q628term | foi-PD; Q628term | foi-PC; Q628term | foi-PA; Q628term | foi-PB

Comment:

Reported as a C to T change at nucleotide 2503 of the foi cDNA, assumed to be RE41071. Mutation mapped to position 2504 of the cDNA, which corresponds to the C of a Glutamine codon.

(Carrasco-Rando et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic midgut constriction

(Carrasco-Rando et al., 2016)

embryonic somatic muscle cell

(Carrasco-Rando et al., 2016)

embryonic somatic muscle cell (with Df(3L)BSC13)

(Carrasco-Rando et al., 2016)

fusion competent cell

(Carrasco-Rando et al., 2016)

gastric caecum primordium

(Carrasco-Rando et al., 2016)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Carrasco-Rando et al., 2016)

muscle cell of dorsal acute muscle 1 | embryonic stage

(Carrasco-Rando et al., 2016)

visceral mesoderm

(Carrasco-Rando et al., 2016)

Detailed Description

Statement

Reference

foi^C887/foi^C887 and foi^C887/Df(3L)BSC13 mutant embryos display defective muscle patterning with missing muscles, morphological defects in dorsal, lateral and ventral groups of muscles, and a fusion defect shown by reduced size of the remaining muscles. In foi^C887/foi^C887 mutant embryos, the visceral mesoderm is defective, with loss of the gastric caeca and anterior gut constriction, misplaced middle constriction, in many cases a delay in the formation or failure to complete the posterior gut constriction, and the DA1 muscle displays myoblast fusion defects, as compared to wild type. Mutants also display failure of the SNb nerve branch to defasciculate from the dorsal ISN leaving ventral muscles without innervation, as compared with controls. Founder cell segregation is unaffected in foi^C887/foi^C887 mutants, but there appears to be failed specification and/or segregation of fusion-competent myoblasts (FCMs), and an increase in the number of apoptotic FCMs.

(Carrasco-Rando et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

foi^C887 has abnormal cell death | embryonic stage phenotype, suppressible by Df(3L)H99/Df(3L)H99

(Carrasco-Rando et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

foi^C887 has fusion competent cell phenotype, suppressible by Zip71B^UAS.cCa/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

foi^C887 has fusion competent cell phenotype, suppressible by Zip42C.1^UAS.EGFP/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

foi^C887 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype, suppressible | partially by Zip42C.1^UAS.EGFP/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

NOT suppressed by

Statement

Reference

foi^C887 has fusion competent cell phenotype, non-suppressible by Df(3L)H99/Df(3L)H99

(Carrasco-Rando et al., 2016)

foi^C887 has fusion competent cell phenotype, non-suppressible by Scer\GAL4^Mef2.PU/ZnT63C^UAS.cWa

(Carrasco-Rando et al., 2016)

foi^C887 has fusion competent cell phenotype, non-suppressible by lmd^UAS.cRa/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

foi^C887 has fusion competent cell phenotype, non-suppressible by Scer\GAL4^Mef2.PU/Catsup^UAS.cCa

(Carrasco-Rando et al., 2016)

foi^C887 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.PU/Catsup^UAS.cCa

(Carrasco-Rando et al., 2016)

foi^C887 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype, non-suppressible by Zip71B^UAS.cCa/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

foi^C887 has fusion competent cell phenotype, non-suppressible by Scer\GAL4^Mef2.PU/Hsap\SLC39A6^UAS.cCa

(Carrasco-Rando et al., 2016)

foi^C887 has muscle cell of dorsal acute muscle 1 | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.PU/Hsap\SLC39A6^UAS.cCa

(Carrasco-Rando et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

foi^C887/foi^C887, Df(3L)H99/Df(3L)H99 double mutants do not exhibit apoptosis of fusion-competent myoblasts (FCMs), but otherwise still exhibit defective differentiation of FCMs and do not appear phenotypically different to foi^C887/foi^C887 mutants.

Expression of ZnT63C^Scer\UAS.cWa or lmd^Scer\UAS.cRa under the control of Scer\GAL4^Mef2.PU fails to rescue the faulty specification of fusion-competent myoblasts in foi^C887/foi^C887 mutants.

Expression of Catsup^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PU fails to rescue the faulty specification of fusion-competent myoblasts nor the DA1 myoblast fusion defect in foi^C887/foi^C887 mutants.

Expression of Zip42C.1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mef2.PU significantly rescues the faulty specification of fusion-competent myoblasts, and partially rescues the DA1 fusion defects of foi^C887/foi^C887 mutants.

Expression of Zip71B^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PU significantly rescues the faulty specification of fusion-competent myoblasts, but does not rescue the myoblast fusion defects, including in the DA1 muscle, of foi^C887/foi^C887 mutants.

(Carrasco-Rando et al., 2016)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\SLC39A6^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PU fails to rescue the faulty specification of fusion-competent myoblasts nor the DA1 myoblast fusion defect in foi^C887/foi^C887 mutants.

(Carrasco-Rando et al., 2016)

Complementation and Rescue Data

Rescued by

foi^C887 is rescued by foi^UAS.cCa/Scer\GAL4^Mef2.PU

(Carrasco-Rando et al., 2016)

Partially rescued by

foi^C887 is partially rescued by Scer\GAL4^sns.PU/foi^UAS.cCa

(Carrasco-Rando et al., 2016)

foi^C887 is partially rescued by foi^UAS.cCa/Scer\GAL4^kirre.PU

(Carrasco-Rando et al., 2016)

foi^C887 is partially rescued by foi^UAS.cCa

(Carrasco-Rando et al., 2016)

Comments

Expression of foi^Scer\UAS.cCa under the control of Scer\GAL4^Mef2.PU fully rescues the muscle patterning and fusion, visceral mesoderm, and defective fusion-competent myoblast differentiation phenotypes of foi^C887/foi^C887 mutants.

Expression of foi^Scer\UAS.cCa under the control of Scer\GAL4^sns.PU partially rescues the defective fusion-competent myoblast differentiation and DA1 fusion phenotypes of foi^C887/foi^C887 mutants.

Expression of foi^Scer\UAS.cCa under the control of Scer\GAL4^kirre.PU fails to rescue the defective fusion-competent myoblast differentiation, but does rescue the muscle patterning defects of foi^C887/foi^C887 mutants.

Expression of foi^Scer\UAS.cCa with no GAL4 driver is sufficient to reduce the defective fusion-competent myoblast differentiation phenotype, and partially rescue the DA1 fusion phenotype of foi^C887/foi^C887 mutants.

(Carrasco-Rando et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

foi^C887

(Carrasco-Rando et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close