FlyBase Allele Report: Zzzz\CAG[250.UAS.DsRed2]

General Information

Symbol

Zzzz\CAG^{250.UAS.DsRed2}

Species

a. artificial

Name

FlyBase ID

FBal0319635

Feature type

allele

Associated gene

Zzzz\CAG

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed2.CAG250}

Transgenic product class

trinucleotide_repeat_expansion

(Li et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Li et al., 2008)

Mutagen

in vitro construct

(Li et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-DsRed2.CAG250}

Cytology

Description

UASt regulatory sequences drive expression of DsRed2 followed by a 3'UTR containing untranslated CAG repeats (repeat length of 250).

(Li et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\CAG

Tagged with

DsRed2

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Peng et al., 2021, Zhang et al., 2018, Shieh and Bonini, 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of neurodegenerative disease

is ameliorated by wech^E546

(Shieh and Bonini, 2011)

is ameliorated by Hsap\HSPA1L^UAS.cWa

(Shieh and Bonini, 2011)

is ameliorated by Tpr2^EB7-1A

(Shieh and Bonini, 2011)

is ameliorated by orb2^B8-S

(Shieh and Bonini, 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Yu et al., 2011)

eye, with Scer\GAL4^GMR.PF, Zzzz\CTG^{200.UAS.DsRed2}

(Yu et al., 2011)

eye, with Scer\GAL4^GMR.PF, Zzzz\CTG^{250.UAS.DsRed2}

(Yu et al., 2011)

eye, with Scer\GAL4^GMR.PF, Zzzz\CTG^{270.UAS.DsRed2}

(Yu et al., 2011)

retina, with Scer\GAL4^GMR.PF

(Yu et al., 2011)

retina, with Scer\GAL4^GMR.PF, Zzzz\CTG^{200.UAS.DsRed2}

(Yu et al., 2011)

retina, with Scer\GAL4^GMR.PF, Zzzz\CTG^{250.UAS.DsRed2}

(Yu et al., 2011)

retina, with Scer\GAL4^GMR.PF, Zzzz\CTG^{270.UAS.DsRed2}

(Yu et al., 2011)

wing, with Scer\GAL4^da.PU

(Shieh and Bonini, 2011)

Detailed Description

Statement

Reference

Expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} under the control of Scer\GAL4^da.PU results in compromised climbing ability at day one that progressively deteriorates over 14 days.

70% of flies expressing Disc\RFP^{CAG250.DsRed2.Scer\UAS} under the control of Scer\GAL4^da.PU display an abnormal wing posture, holding one or both wings up.

(Shieh and Bonini, 2011)

Expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} in the developing eye, under the control of Scer\GAL4^GMR.PF gives a weak rough eye phenotype, with some abnormal eye pigmentation and disruption of retinal integrity.

Co-expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} and Disc\RFP^{(CTG)250.Scer\UAS} in the eye, under the control of Scer\GAL4^GMR.PF results in late developmental stage lethality. Dissection of animals from the pupal case reveals severely disrupted eye morphology.

Co-expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} and Disc\RFP^{(CTG)200.Scer\UAS} in the eye, under the control of Scer\GAL4^GMR.PF, results in dramatic toxicity: the eye becomes severely rough with abnormal pigmentation and a severe loss of retinal integrity. This effect is synergistic as expression of either two copies of Disc\RFP^{CAG250.DsRed2.Scer\UAS} or Disc\RFP^{(CTG)200.Scer\UAS} alone has limited or no effect.

Co-expression of Disc\RFP^{(CTG)270.Scer\UAS} with Disc\RFP^{CAG250.DsRed2.Scer\UAS} in the developing eye under the control of Scer\GAL4^GMR.PF leads to a disrupted eye externally, with severe loss of retinal integrity internally.

Adult flies with a 30 minute heat shock induction of Disc\RFP^{CAG250.DsRed2.Scer\UAS} (under the control of Scer\GAL4^hs.PB) are viable and show no ill effects.

Adult flies with a 30 minute heat shock induction of Disc\RFP^{CAG250.DsRed2.Scer\UAS} and Disc\RFP^{(CTG)250.Scer\UAS} (both under the control of Scer\GAL4^hs.PB) start to day within 24 hours, with approximately 90% of flies dead after 50 hours.

(Yu et al., 2011)

Flies expressing Disc\RFP^{CAG250.DsRed2.Scer\UAS} in neurons under the control of Scer\GAL4^elav-C155 die early with progressive loss of climbing ability compared to controls.

(Li et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, Zzzz\CAG^{250.UAS.DsRed2} has short lived phenotype, enhanceable by Hsap\MBNL1^UAS.cGCa, Scer\GAL4^elav-C155

(Li et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{250.UAS.DsRed2}, Zzzz\CTG^{250.UAS.DsRed2} has increased mortality during development phenotype, suppressible by Dcr-2^L811fsX

(Yu et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Zzzz\CAG^{250.UAS.DsRed2}, Zzzz\CTG^{200.UAS.DsRed2} has eye phenotype, suppressible by AGO2⁴¹⁴

(Yu et al., 2011)

Scer\GAL4^GMR.PF, Zzzz\CAG^{250.UAS.DsRed2}, Zzzz\CTG^{200.UAS.DsRed2} has retina phenotype, suppressible by AGO2⁴¹⁴

(Yu et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\HSPA1L^Scer\UAS.cWa suppresses the climbing defects seen when Disc\RFP^{CAG250.DsRed2.Scer\UAS} is expressed under the control of Scer\GAL4^da.PU.

Expression of Tpr2^EB7-1A partially suppresses the wing posture and climbing defects seen when Disc\RFP^{CAG250.DsRed2.Scer\UAS} is expressed under the control of Scer\GAL4^da.PU.

Expression of wech^E546 partially suppresses the wing posture and climbing defects seen when Disc\RFP^{CAG250.DsRed2.Scer\UAS} is expressed under the control of Scer\GAL4^da.PU.

Expression of orb2^B8-S partially suppresses the wing posture and climbing defects seen when Disc\RFP^{CAG250.DsRed2.Scer\UAS} is expressed under the control of Scer\GAL4^da.PU.

(Shieh and Bonini, 2011)

A Dcr-2^L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} and Disc\RFP^{(CTG)250.Scer\UAS} (under the control of Scer\GAL4^hs.PB).

A Dcr-2^L811fsX homozygous mutant background has minimal effect on the toxicity associated with expression of Disc\RFP^{CAG250.DsRed2.Scer\UAS} in the muscle (under the control of Scer\GAL4^how-24B).

An AGO2⁴¹⁴ homozygous background dramatically mitigates the toxicity found upon co-expression of Disc\RFP^{(CTG)200.Scer\UAS} and Disc\RFP^{CAG250.DsRed2.Scer\UAS} in the developing eye, under the control of Scer\GAL4^GMR.PF.

(Yu et al., 2011)

Co-expression of Hsap\MBNL1^{Scer\UAS.cGCa} further shortens the lifespan of flies expressing Disc\RFP^{CAG250.DsRed2.Scer\UAS} pan-neuronally under the control of Scer\GAL4^elav-C155.

(Li et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Disc\RFP^{CAG250.DsRed2.Scer\UAS}

Zzzz\CAG^{250.Scer\UAS.T:Disc\RFP-DsRed2}

Zzzz\CAG^{250.UAS.DsRed2}

Name Synonyms

Secondary FlyBase IDs

FBal0319580
FBal0283320

References (6)

Report Sections

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