Expression of Disc\RFP(CTG)130.Scer\UAS in the muscle (Scer\GAL4how-24B) does not affect viability. However, expression in the nervous system (Scer\GAL4elav-C155) results in pupal lethality. Ubiquitous expression (Scer\GAL4da.G32) of Disc\RFP(CTG)130.Scer\UAS results in larval lethality.
Expression of Disc\RFP(CTG)200.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF gives a moderate rough eye phenotype, with abnormal eye pigmentation and disruption of retinal integrity.
Scer\GAL4GMR.PF, Zzzz\CAG250.UAS.DsRed2, Zzzz\CTG200.UAS.DsRed2 has eye phenotype, suppressible by AGO2414
Scer\GAL4GMR.PF, Zzzz\CAG250.UAS.DsRed2, Zzzz\CTG200.UAS.DsRed2 has retina phenotype, suppressible by AGO2414
An AGO2414 homozygous background dramatically mitigates the toxicity found upon co-expression of Disc\RFP(CTG)200.Scer\UAS and Disc\RFPCAG250.DsRed2.Scer\UAS in the developing eye, under the control of Scer\GAL4GMR.PF.