FB2024_03 , released June 25, 2024
Allele: Zzzz\CTG250.UAS.DsRed2
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General Information
Symbol
Zzzz\CTG250.UAS.DsRed2
Species
a. artificial
Name
FlyBase ID
FBal0319642
Feature type
allele
Associated gene
Zzzz\CTG
Associated Insertion(s)
Carried in Construct
P{UAS-DsRed-CTG250}
Transgenic product class
trinucleotide_repeat_expansion
(Yu et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
trinucleotide_repeat_expansion
(Yu et al., 2011)
Mutagen
in vitro construct
(Yu et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-DsRed-CTG250}
Cytology
Description

UASt regulatory sequences drive expression of DsRed2 coding sequences, with a CTG sequence repeated an uninterrupted 250 times in the 3'UTR.

(Yu et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Zzzz\CTG
Tagged with
DsRed2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Yu et al., 2011)
      DMPK:n.3_prime_UTR[250CUG]
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      DMPK, (CTG)n REPEAT EXPANSION, 3-PRIME UTR
      DMPK:n.3_prime_UTR[250CTG]
      Associated human disease model(s)
      External database links
      ClinVar: 5049
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Disc\RFP(CTG)250.Scer\UAS in the muscle (Scer\GAL4how-24B) results in some lethality. Expression either ubiquitously (Scer\GAL4da.G32) or in the nervous system (Scer\GAL4elav-C155) results in larval lethality.

      Adult flies with a 30 minute heat shock induction of Disc\RFPCAG250.DsRed2.Scer\UAS (under the control of Scer\GAL4hs.PB) are viable and show no ill effects.

      Adult flies with a 30 minute heat shock induction of Disc\RFP(CTG)250.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (both under the control of Scer\GAL4hs.PB) start to day within 24 hours, with approximately 90% of flies dead after 50 hours.

      (Yu et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      A Dcr-2L811fsX homozygous mutant background suppresses the organismal lethality associated with 30 minute heat-shock induced expression of Disc\RFPCAG250.DsRed2.Scer\UAS and Disc\RFP(CTG)250.Scer\UAS (under the control of Scer\GAL4hs.PB).

      A Dcr-2L811fsX homozygous mutant background has minimal effect on the toxicity associated with expression of Disc\RFP(CTG)250.Scer\UAS in the muscle (under the control of Scer\GAL4how-24B).

      (Yu et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Disc\RFP(CTG)250.Scer\UAS
      Zzzz\CTG250.Scer\UAS.T:Disc\RFP
      Zzzz\CTG250.UAS.DsRed2
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0319587
      • FBal0285128
      References (3)