FB2024_03 , released June 25, 2024
Allele: Dmel\lncRNA:let7CKO2
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General Information
Symbol
Dmel\lncRNA:let7CKO2
Species
D. melanogaster
Name
FlyBase ID
FBal0297476
Feature type
allele
Associated gene
Dmel\lncRNA:let7C
Associated Insertion(s)
TI{TI}lncRNA:let7CKO2
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Wu et al., 2012)
Mutagen
FLPase
(Wu et al., 2012)
I-SceI
(Wu et al., 2012)
ends-out gene targeting
(Wu et al., 2012)
Progenitor genotype
Associated Insertion(s)
TI{TI}lncRNA:let7CKO2
Caused by aberration
Df(2L)let-7-CKO2
Cytology
Description

The endogenous let-7-C locus is replaced with w, resulting in deletion of mir-100, let-7 and mir-125.

(Wu et al., 2012)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal locomotor behavior | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-125

      (Chawla et al., 2016)

      abnormal locomotor behavior | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-let7

      (Chawla et al., 2016)

      abnormal locomotor behavior | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmiRNAs, lncRNA:let7C3.3

      (Chawla et al., 2016)

      abnormal neuroanatomy (with lncRNA:let7CKO1)

      (Wu et al., 2012)

      abnormal neuroanatomy | adult stage (with lncRNA:let7CKO1), with lncRNA:let7CΔmir-125

      (Chawla et al., 2016)

      abnormal neuroanatomy | adult stage (with lncRNA:let7CKO1), with lncRNA:let7CΔmir-let7

      (Chawla et al., 2016)

      abnormal neuroanatomy | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7C+tCa, lncRNA:let7C3.3

      (Chawla et al., 2016)

      abnormal neuroanatomy | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-125

      (Chawla et al., 2016)

      abnormal neuroanatomy | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-let7

      (Chawla et al., 2016)

      abnormal neuroanatomy | adult stage | progressive (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmiRNAs, lncRNA:let7C3.3

      (Chawla et al., 2016)

      abnormal neuroanatomy | dominant

      (Wu et al., 2012)

      abnormal neuroanatomy | somatic clone

      (Wu et al., 2012)

      decreased cell number | adult stage (with lncRNA:let7CKO1), with lncRNA:let7CΔmir-125

      (Chawla et al., 2016)

      decreased cell number | adult stage (with lncRNA:let7CKO1), with lncRNA:let7CΔmir-let7

      (Chawla et al., 2016)

      short lived (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-125

      (Chawla et al., 2016)

      short lived (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmir-let7

      (Chawla et al., 2016)

      short lived (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmiRNAs

      (Chawla et al., 2016)

      short lived (with Df(2L)let-7-CGKI), with lncRNA:let7CΔmiRNAs, lncRNA:let7C3.3

      (Chawla et al., 2016)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The following genotypes results in short lived adults that exhibit a progressive reduction in climbing ability and a progressive brain vacuolization: lncRNA:let7CKO2/Df(2L)let-7-CGKI, lncRNA:let7CΔmiRNAs, lncRNA:let7C3.3; lncRNA:let7CKO2/Df(2L)let-7-CGKI, lncRNA:let7CΔmir-let7; lncRNA:let7CKO2/Df(2L)let-7-CGKI, lncRNA:let7CΔmir-125.

      Adults transheterozygous for lncRNA:let7CKO2/Df(2L)let-7-CGKI and also bearing lncRNA:let7C+tCa and lncRNA:let7C3.3 exhibit a progressive brain vacuolization.

      Adults transheterozygous for lncRNA:let7CKO2/lncRNA:let7CKO1 and also bearing lncRNA:let7CΔmir-let7 or lncRNA:let7CΔmir-125 exhibit a decrease in the number of pioneer α/β mushroom body neurons.

      (Chawla et al., 2016)

      let-7-CKO2 mutant neurons display delays in the α'/β' to p.α/β and p.α/β to α/β transitions in mushroom body neuron identities during neurogenesis, compared to wild type. Consistent with this, let-7-CKO1/let-7-CKO2 adults show an increased number of earlier born α'/&bgr' neurons, and a decreased number of later born p.α/β neurons. While the morphology of mutant mushroom bodies is grossly normal, some p.α/β and α/β axons inappropriately cross the midline.

      let-7-CKO2/+ adults show a reduction in p.α/β mushroom body neurons.

      (Wu et al., 2012)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      NOT Enhancer of
      Statement
      Reference

      lncRNA:let7CKO2 is a non-enhancer of abnormal neuroanatomy | somatic clone phenotype of chinmo1

      (Wu et al., 2012)
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Df(2L)let-7-CGKI/lncRNA:let7CKO2, lncRNA:let7CΔmir-let7 has adult brain phenotype, non-suppressible by chinmo1

      (Chawla et al., 2016)
      NOT Enhancer of
      Statement
      Reference

      lncRNA:let7CKO2 is a non-enhancer of mushroom body | somatic clone phenotype of chinmo1

      (Wu et al., 2012)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduction in p.α/β mushroom body neurons in let-7-CKO2/+ adults is restored to normal numbers by chinmo1/+.

      chinmo1; let-7-CKO2 double mutants neurons aberrantly acquire late-type neurite trajectories, as displayed by chinmo1 single mutant neurons. Mushroom bodies generated from double mutant neuroblasts appear identical to those generated from chinmo1 single mutants.

      (Wu et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer

      Generated in parallel with Df(2L)let-7-CGKI and is identical to it except that the endogenous let-7-C locus is replaced with w rather than w and Scer\GAL4.

      (Wu et al., 2012)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      let-7-CKO2
      (Chawla et al., 2016)
      lncRNA:let7CKO2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)