FlyBase Allele Report: Dmel\chinmo[1]

General Information

Symbol

Dmel\chinmo¹

Species

D. melanogaster

Name

FlyBase ID

FBal0197456

Feature type

allele

Associated gene

Dmel\chinmo

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA with brat¹¹

(Narbonne-Reveau et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS

(Enomoto et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape | somatic clone

(Lin et al., 2012)

abnormal neuroanatomy | somatic clone

(Lin et al., 2012, Wu et al., 2012, Yu et al., 2009, Zhu et al., 2006)

abnormal size | adult stage | somatic clone - Minute background, with Scer\FLP1^ey.PU

(Flaherty et al., 2010)

decreased body size | embryonic stage (with Df(2L)Exel6005)

(Truman and Riddiford, 2022)

lethal (with chinmo^M33)

(Ma et al., 2014)

lethal (with Df(2L)ast2)

(Ma et al., 2014)

lethal (with Df(2L)BSC480)

(Ma et al., 2014)

lethal (with Df(2L)BSC521)

(Ma et al., 2014)

lethal (with Df(2L)dp-79b)

(Ma et al., 2014)

lethal (with Df(2L)ED7762)

(Ma et al., 2014)

lethal (with Df(2L)Exel6005)

(Ma et al., 2014)

lethal (with Df(2L)S2)

(Ma et al., 2014)

majority die during P-stage | somatic clone - Minute background, with Scer\FLP1^ey.PU

(Flaherty et al., 2010)

partially lethal - majority die | somatic clone - Minute background, with Scer\FLP1^ey.PU

(Flaherty et al., 2010)

some die during embryonic stage

(Truman and Riddiford, 2022)

some die during embryonic stage (with Df(2L)BSC521)

(Truman and Riddiford, 2022)

some die during embryonic stage (with Df(2L)Exel6005)

(Truman and Riddiford, 2022)

Phenotype Manifest In

adult antennal lobe local neuron lLN2 | somatic clone

(Lin et al., 2012)

adult antennal lobe projection neuron DM1 lPN | somatic clone

(Lin et al., 2012)

adult antennal lobe projection neuron DM2 lPN | somatic clone

(Lin et al., 2012)

adult antennal lobe projection neuron VC1 lPN | somatic clone

(Lin et al., 2012)

adult antennal lobe projection neuron VC2 lPN | somatic clone

(Lin et al., 2012)

adult head | somatic clone - Minute background, with Scer\FLP1^ey.PU

(Flaherty et al., 2010)

cyst progenitor cell | adult stage | somatic clone

(Flaherty et al., 2010)

larval mushroom body & neuroblast | somatic clone

(Zhu et al., 2006)

mushroom body | somatic clone

(Wu et al., 2012)

neuroblast | somatic clone

(Yu et al., 2009)

pioneer neuron | somatic clone

(Zhu et al., 2006)

somatic cell of testis | adult stage (with chinmo^KG05386)

(Ma et al., 2014)

somatic cell of testis | adult stage (with chinmo^ST)

(Ma et al., 2014)

testis | adult stage (with chinmo^KG05386)

(Ma et al., 2014)

testis | adult stage (with chinmo^ST)

(Ma et al., 2014)

Detailed Description

Statement

Reference

Arrested chinmo¹/Df(2L)Exel6005 embryos appear short along the anterior-posterior axis.

(Truman and Riddiford, 2022)

chinmo^M33/chinmo¹, chinmo¹/Df(2L)S2, chinmo¹/Df(2L)ast2, chinmo¹/Df(2L)dp-79b, chinmo¹/Df(2L)Exel6005, chinmo¹/Df(2L)ED7762, chinmo¹/Df(2L)BSC480, or chinmo¹/Df(2L)BSC521 mutants are lethal.

chinmo¹/chinmo¹ mutant somatic cyst stem cell clones are lost from the adult testis stem cell niche and testes do not exhibit any signs of sex transformation.

chinmo^ST/chinmo¹ or chinmo^KG05386/chinmo¹ mutant males exhibit somatic cells with a follicle cell-like phenotype.

(Ma et al., 2014)

Some earlier-born lateral antennal lobe uniglomerular projection neurons (VC2, VC1, DM1 and DM2) of chinmo¹ homozygous antennal lobe lateral neuroblast clones adopt later-born morphologies and chinmo¹/chinmo¹ LN2 local neurons adopt the next-born, LN3, fate.

(Lin et al., 2012)

chinmo¹ mushroom body neurons aberrantly acquire late-type neurite trajectories.

(Wu et al., 2012)

Few escapers with eye-antennal disc chinmo¹ clones (in the Minute background) exhibit often only a rudimentary head when compared to controls.

At 2 days post-clone induction (pci), chinmo¹ germline stem cell (GSC, Vasa staining) clones are in contact with the Hub (a group of somatic cells in testis) and cyst stem cell (CySC, Zfh1 or Tj staining) clones envelope germ line cells similar to control MARCM and flip-out clones. At 7 days pci, unlike controls, there isn't any CySc clones in contact with the Hub but there are Tj+ somatic clones outside of the Hub while there are numerous GSC clones in contact with the Hub similar to controls.

(Flaherty et al., 2010)

Knocking out chinmo using chinmo¹ somatic clones in the GMC of the central complex sublineage causes the third sibling to exhibit a temporal cell fate similar to that of the fourth neuron in the wild-type lineage. Neurons derived from chinmo¹ mutant third GMCs, as determined by associated wild-type neuroblast clones containing three cells, acquire projections that are characteristic of wild-type four siblings. They innvervate the middle subcompartment of the noduli with the wild-type sibling of the neuroblast clone. In addition, mutant third siblings, similar to wild-type fourth-born neurons, acquite a much more diffuse pattern of neurite elaboration in the lateral accessory lobe than wild-type third-born neurons.

chinmo¹ mutant neuroblast clones, induced during production of the second GMC, do not produce detectable axons in the ventral subcompartment of the noduli.

(Yu et al., 2009)

Mushroom body neuroblast clones homozygous for chinmo¹ generated at newly hatched larval stage contains only one vertical and one horizontal axon bundle despite presence of many cell bodies. Mutant neuroblast clones also contain ectopic bundles of axons that exhibit a high affinity for the mAb Fas2, suggesting that most of the axons in chinmo¹ mutant neuroblasts likely differentiate as α/β type of mushroom body projections.

There appear to be far more projections of the pioneer α/β type in chinmo¹ mutant neuroblast clones, with qualitatively fewer γ type neurons.

Many chinmo¹ mutant mushroom body single cell clones, despite being derived during early larval development, aberrantly acquire late-type neurite trajectories. For example, following induction of mitotic recombination at two days after larval hatching, γ-type neurons are obtained in only one-third of the single-cell mushroom body clones (as oppose to 100% in wild-type), while many more single-cell mushroom body clones that have both vertical and horizontal projections, and that appear to be either α'/β' or pioneer α/β neurons, are obtained. Furthermore, only α'/β and pioneer α/β neurons are detected among the single-cell clones that are induced at three days after larval hatching.

chinmo¹ heterozygous larvae exhibit α'/β' neurons in chinmo¹ single-cell/two-cell clones induced at as early as three days after larval hatching and exhibit a higher percentage of α'/β neurons (approximately 45%) at the 3.25 day after larval hatching induction than the wild-type control (approximately 36%) at the 3.5 day after larval hatching induction. This indicates that, as compared to wild-type, α'/β' neurons are generated at least 0.25 days earlier in the chinmo¹/+ heterozygous animals. In addition, pioneer α/β neurons are also born precociously in a chinmo¹/+ heterozygous background, as evidenced by the phenomenon that 47% (compared to 4% in wild-type) of the single-cell/two-cell clones, induced within 12-6hr before pupal formation, develop into pioneer α/β neurons in chinmo¹/+ heterozygotes.

Approximately 80 pioneer α/β neurons are present in chinmo¹/+ adult flies, compared to an average of 60 in wild-type.

Labeled chinmo¹ single-cell/two-cell clones, induced in newly hatched larvae, innervate the D, instead of DL1, glomeruli. They also exhibit an axon arborization pattern resembling that of wild-type D-targeting projection neurons. Thus, chinmo¹ mutant projection neurons, born in newly hatched larvae, apparently develop into the D-targeting projection neurons that also belong to the anterodorsal lineage, but are normally not generated until about two days after larval hatching. In contrast, chinmo¹ is dispensable for specification of late-born cell fates, with both chinmo¹ mutant, and wild-type clones, sharing the same morphological features of late-born projection neurons: all clones elaborated dendrites in both DM6 and VA1lm glomeruli.

(Zhu et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

chinmo¹ has abnormal neuroanatomy | somatic clone phenotype, non-enhanceable by lncRNA:let7C^KO2

(Wu et al., 2012)

Suppressor of

Statement

Reference

chinmo¹ is a suppressor | partially of short lived phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-let7

(Chawla et al., 2016)

chinmo¹ is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-let7

(Chawla et al., 2016)

chinmo¹ is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-125

(Chawla et al., 2016)

chinmo¹ is a suppressor | partially of short lived phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-125

(Chawla et al., 2016)

chinmo¹ is a suppressor | partially of abnormal locomotor behavior | adult stage | progressive phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-125

(Chawla et al., 2016)

chinmo[+]/chinmo¹ is a suppressor of abnormal neuroanatomy | dominant phenotype of lncRNA:let7C^KO1

(Wu et al., 2012)

chinmo[+]/chinmo¹ is a suppressor of abnormal neuroanatomy | dominant phenotype of lncRNA:let7C^KO2

(Wu et al., 2012)

NOT Suppressor of

Statement

Reference

chinmo¹ is a non-suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-let7

(Chawla et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

chinmo¹ has mushroom body | somatic clone phenotype, non-enhanceable by lncRNA:let7C^KO2

(Wu et al., 2012)

NOT suppressed by

Statement

Reference

chinmo¹ has cyst progenitor cell | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/BacA\p35^UAS.cHa

(Flaherty et al., 2010)

chinmo¹ has cyst progenitor cell | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/zfh1^UAS.cPa

(Flaherty et al., 2010)

Suppressor of

Statement

Reference

chinmo¹ is a suppressor of lipid droplet | adult stage phenotype of mir-125^Δ.cSa

(Pandey et al., 2021)

chinmo¹ is a suppressor of adult fat body | adult stage phenotype of mir-125^Δ.cSa

(Pandey et al., 2021)

chinmo¹ is a suppressor of adult brain phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-125

(Chawla et al., 2016)

chinmo[+]/chinmo¹ is a suppressor of mushroom body phenotype of lncRNA:let7C^KO1

(Wu et al., 2012)

chinmo[+]/chinmo¹ is a suppressor of mushroom body phenotype of lncRNA:let7C^KO2

(Wu et al., 2012)

NOT Suppressor of

Statement

Reference

chinmo¹ is a non-suppressor of adult brain phenotype of Df(2L)let-7-C^GKI/lncRNA:let7C^KO2, lncRNA:let7C^Δmir-let7

(Chawla et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in p.α/β mushroom body neurons in let-7-C^KO1/+ or let-7-C^KO2/+ adults is restored to normal numbers by chinmo¹/+.

chinmo¹; let-7-C^KO2 double mutants neurons aberrantly acquire late-type neurite trajectories, as displayed by chinmo¹ single mutant neurons. Mushroom bodies generated from double mutant neuroblasts appear identical to those generated from chinmo¹ single mutants.

(Wu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

chinmo¹ is rescued by chinmo^UAS.fl/Scer\GAL4^ey-OK107

(Zhu et al., 2006)

Comments

Expression of chinmo^Scer\UAS.fl, under the control of Scer\GAL4^OK107 grossly rescues the phenotypes characteristic of chinmo¹ mutant mushroom body neuroblast clones.

(Zhu et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59969

w^*; chinmo¹ P{neoFRT}40A/CyO, y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Chinmo¹

(Lin et al., 2012)

chinmo¹

(Truman and Riddiford, 2022, Enomoto et al., 2021, Pandey et al., 2021, Wu et al., 2020, Liu et al., 2019, Marchetti and Tavosanis, 2017, Ren et al., 2017, Syed et al., 2017, Chawla et al., 2016, Narbonne-Reveau et al., 2016, Ma et al., 2014, Kao et al., 2012, Wu et al., 2012, Flaherty et al., 2010, Yu et al., 2009, Zhu et al., 2006)

l(2L)MB523

(Zhu et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (17)

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