FlyBase Allele Report: Dmel\Sarm[896]

General Information

Symbol

Dmel\Sarm⁸⁹⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0277677

Feature type

allele

Associated gene

Dmel\Sarm

Associated Insertion(s)

Carried in Construct

Also Known As

dsarm⁸⁹⁶

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: Q603term.

(Osterloh et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:8,103,298..8,103,298 [+]

Nucleotide change:

C8103298T

Amino acid change:

Reported amino acid change:

Q603term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Osterloh et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | adult stage

(Neukomm et al., 2017)

axon | adult stage | somatic clone

(Hsu et al., 2021, Neukomm et al., 2017)

wing nerve | somatic clone

(Hsu et al., 2021)

wing vein L1 | somatic clone | conditional

(Neukomm et al., 2014)

Detailed Description

Statement

Reference

Flies carrying Ect4⁸⁹⁶ mutant somatic clones show defective injury-induced axon degeneration (severed sensory neuron axons in the adult wing remain intact for a full week after an axotomy, instead of being cleared away by day 5). After axotomy of mechanosensory neurons in the Johnston's organ, the persisting severed axons can still elicit grooming behavior upon optogenetic stimulation of the neurons.

(Neukomm et al., 2017)

Ect4⁸⁹⁶ has a strong protective effect on L1 vein neurons following axotomy. Whereas wild type axons undergo fragmentation, many severed but intact axons are observed.

(Neukomm et al., 2014)

The axons of homozygous olfactory receptor neurons remain intact 1 week after axon severing (in contrast to wild type). The mutant axons remain fully intact 30 days after axon severing, and a significant but reduced number remain intact 50 days after axon severing.

Pruning of axons and dendrites during metamorphosis occurs normally in homozygous mushroom body gamma neuron clones.

(Osterloh et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Sarm⁸⁹⁶ is a suppressor | partially of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

Sarm⁸⁹⁶ is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a non-suppressor of abnormal neuroanatomy | somatic clone phenotype of Hsap\TARDBP^{Q331K.UAS.cSa}, Scer\GAL4^VGlut-OK371

(Sreedharan et al., 2015)

Sarm⁸⁹⁶ is a non-suppressor of visible phenotype of hid^GMR.PG

(Osterloh et al., 2012)

Other

Statement

Reference

Sarm^{ΔARM.UAS.Tag:MYC}, Sarm⁸⁹⁶, Scer\GAL4^VGlut-OK371 has abnormal neuroanatomy | adult stage phenotype

(Neukomm et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Sarm⁸⁹⁶ is a suppressor | partially of axon | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a suppressor | partially of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^GD8082

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a suppressor | partially of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

Sarm⁸⁹⁶ is a non-suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a non-suppressor of axon | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a non-suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is a non-suppressor of leg | somatic clone phenotype of Hsap\TARDBP^{Q331K.UAS.cSa}, Scer\GAL4^VGlut-OK371

(Sreedharan et al., 2015)

Sarm⁸⁹⁶ is a non-suppressor of motor neuron | somatic clone phenotype of Hsap\TARDBP^{Q331K.UAS.cSa}, Scer\GAL4^VGlut-OK371

(Sreedharan et al., 2015)

Sarm⁸⁹⁶ is a non-suppressor of eye phenotype of hid^GMR.PG

(Osterloh et al., 2012)

Other

Statement

Reference

Sarm^{ΔARM.UAS.Tag:MYC}, Sarm⁸⁹⁶, Scer\GAL4^VGlut-OK371 has axon | adult stage phenotype

(Neukomm et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Ect4⁸⁹⁶ mutant MARCM clones in sensory neurons in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^VGlut-OK371 undergo spontaneous cell body and axon degeneration.

The axon and cell body degeneration in sensory neuron clones in the adult wing mutant for Nmnat^Δ4790-1 or expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 can be fully rescued by combination with axed²⁰⁹⁴ and Ect4⁸⁹⁶ together, but not - or only weakly - by combination with Ect4⁸⁹⁶ alone.

(Neukomm et al., 2017)

Ect4⁸⁹⁶ does not suppress the degeneration seen in leg motor neuron clones expressing Hsap\TARDBP^{Q331K.Scer\UAS.cSa} under the control of Scer\GAL4^VGlut-OK371.

(Sreedharan et al., 2015)

Homozygous Ect4⁸⁹⁶ clones fail to suppress the cell death in the eye caused by W^GMR.PG.

(Osterloh et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

axed⁰⁰¹¹

(Neukomm et al., 2017)

axed²⁰⁹⁴

(Neukomm et al., 2017)

Rescued by

Sarm⁸⁹⁶ is rescued by Sarm^+tOa

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is rescued by Sarm^{ΔTIR.UAS.EGFP}/Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Df(3L)BSC795/Sarm⁸⁹⁶ is rescued by Sarm^+tOa

(Osterloh et al., 2012)

Sarm⁴⁶²¹/Sarm⁸⁹⁶ is rescued by Sarm^+tOa

(Osterloh et al., 2012)

Sarm⁸⁹⁶ is rescued by Scer\GAL4^Or22a.7.717/Sarm^UAS.cOa

(Osterloh et al., 2012)

Partially rescued by

Sarm⁸⁹⁶ is partially rescued by Scer\GAL4^VGlut-OK371/Sarm^UAS.cOa

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is partially rescued by Sarm^UAS.EGFP/Scer\GAL4^VGlut-OK371

(Neukomm et al., 2017)

Not rescued by

Sarm⁸⁹⁶ is not rescued by Scer\GAL4^VGlut-OK371/Sarm^{ΔSAM.UAS.EGFP}

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is not rescued by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.ΔSAM.UAS.EGFP}

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is not rescued by Scer\GAL4^VGlut-OK371/Sarm^{ΔARM.ΔTIR.UAS.Tag:HA}

(Neukomm et al., 2017)

Sarm⁸⁹⁶ is not rescued by Scer\GAL4^VGlut-OK371/Sarm^{ΔSAM.ΔTIR.UAS.EGFP}

(Neukomm et al., 2017)

Comments

Ect4⁸⁹⁶ complements the lethality of either axed²⁰⁹⁴ or axed⁰⁰¹¹ mutants.

The axon death defect (severed sensory neuron axons in the adult wing persist after an axotomy, instead of being cleared away) observed in flies carrying Ect4⁸⁹⁶ neuronal mutant clones can be rescued by combination with Ect4^+tOa or by Scer\GAL4^VGlut-OK371-driven expression of either Ect4^{ΔARM.ΔTIR.Scer\UAS.T:Ivir\HA1}, and partially rescued by expression of Ect4^Scer\UAS.cOa or Ect4^{Scer\UAS.T:Avic\GFP-EGFP} but cannot be rescued by expression of any of the following: Ect4^{ΔSAM.Scer\UAS.T:Avic\GFP-EGFP}, Ect4^{ΔTIR.Scer\UAS.T:Avic\GFP-EGFP}, Ect4^{ΔARM.ΔSAM.Scer\UAS.T:Avic\GFP-EGFP} or Ect4^{ΔSAM.ΔTIR.Scer\UAS.T:Avic\GFP-EGFP} in the mutant clones.

(Neukomm et al., 2017)

Expression of Ect4^Scer\UAS.cOa under the control of Scer\GAL4^Or22a.7.717 reverts the suppression of axonal degeneration after axon severing which is seen in Ect4⁸⁹⁶ mutant neurons.

Ect4^+tOa rescues the lethality and reverts the suppression of axonal degeneration after axon severing which is seen in Ect4⁸⁹⁶/Df(3L)BSC795 and Ect4⁸⁹⁶/Ect4⁴⁶²¹ animals.

(Osterloh et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Ect4⁸⁹⁶

Sarm⁸⁹⁶

dSARM⁸⁹⁶

(Herrmann et al., 2022)

dSarm⁸⁹⁶

(Farley et al., 2018)

dsarm⁸⁹⁶

(Llobet Rosell et al., 2022, Hsu et al., 2021, Neukomm et al., 2017, Sreedharan et al., 2015)

l(3)896

(Osterloh et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (8)

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