FlyBase Allele Report: Dmel\Marf[JF01650]

The expression of Marf^JF01650 under the control of Scer\GAL4^dome-PG14 leads to the blood progenitors in the third instar larva lymph gland to show a decreased mitochondria footprint, with mitochondria displaying fewer, shorter branches and fewer junctions. The lymph gland shows an increase in prohemocyte number in the tertiary lobe, an increase in the number of plasmatocytes in the secondary lobe, and an increase in crystal cells in the secondary and tertiary lobes.

Expression under the control of Scer\GAL4^e33C leads to an increase in crystal cells in the primary lobe of the third instar larval lymph gland.

(Ray et al., 2021)

Expressing Marf^JF01650 under the control of Scer\GAL4^Cg.PA leads to fragmented mitochondria in the third instar larval fat body (as assessed by MitoGFP).

(Zhou et al., 2019)

Wild-type flies upon infection with Serratia marcescens display thinning of intestinal epithelium due to cytoplasm extrusion and accompanied by accumulation of megamitochondria, however in flies expressing Marf^JF01650 under the control of Scer\GAL4^NP3084 (together with tub-Gal80[ts] to limit the time of expression) that do not form megamitochondria the epithelium thinning is still observed.

(Lee et al., 2016)

Expression of Marf^JF01650 driven by Scer\GAL4^Cg.PA results in fragmented, swollen mitochondria that lose most of their cristae.

(Zhang et al., 2016)

Flies expressing Marf^JF01650 under the control of Scer\GAL4^GMR.PU do not exhibit any discernible eye phenotype, as compared to controls.

(Hwang et al., 2014)

Expression of Marf^JF01650 under the control of Scer\GAL4^Bx-MS1096 does not result in a wing phenotype.

(Ratnaparkhi, 2013)

Expression of Marf^JF01650 under the control of Scer\GAL4^elav.PU has no effect on mitochondrial length in adult neurons. The amount of cell death and cell cycle activation are similar to controls, as are levels of superoxide production.

(Duboff et al., 2012)

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Marf^JF01650, Scer\GAL4^e33C has increased cell number | third instar larval stage phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 has increased cell number | third instar larval stage phenotype, enhanceable by asrij^GD2167, Scer\GAL4^dome-PG14

(Ray et al., 2021)

Enhancer of

Statement

Reference

Marf^JF01650, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is an enhancer of increased cell number | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Scer\GAL4^e33C/Marf^JF01650 is an enhancer of increased cell number | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^Cg.PA is an enhancer of abnormal size | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Marf^JF01650, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Marf^JF01650, Scer\GAL4^hs.2sev is an enhancer of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Suppressor of

Statement

Reference

Marf^JF01650, Scer\GAL4^elav.PU is a suppressor of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^JF01650, Scer\GAL4^elav.PU is a suppressor of abnormal cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^JF01650, Scer\GAL4^elav.PU is a suppressor of abnormal cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev, BacA\p35^GMR.PH/Marf^JF01650 is a non-suppressor of increased cell death phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Marf^JF01650, Scer\GAL4^dome-PG14 has embryonic/larval crystal cell | third instar larval stage | increased number phenotype, enhanceable by asrij^GD2167, Scer\GAL4^dome-PG14

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 has embryonic/larval lymph gland | third instar larval stage phenotype, enhanceable by asrij^GD2167, Scer\GAL4^dome-PG14

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^e33C has embryonic/larval crystal cell | third instar larval stage | increased number phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^e33C has embryonic/larval lymph gland | third instar larval stage phenotype, enhanceable by asrij⁹/asrij⁹

(Ray et al., 2021)

Suppressed by

Statement

Reference

Marf^JF01650, Scer\GAL4^Cg.PA has mitochondrion phenotype, suppressible | partially by Miga^UAS.RFP(Unk), Scer\GAL4^Cg.PA

(Zhang et al., 2016)

Enhancer of

Statement

Reference

Marf^JF01650, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is an enhancer of embryonic/larval crystal cell | third instar larval stage | increased number phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Scer\GAL4^e33C/Marf^JF01650 is an enhancer of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Marf^JF01650 is an enhancer of primary lobe of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^Cg.PA is an enhancer of mitochondrion | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Marf^JF01650, Scer\GAL4^Cg.PA is an enhancer of embryonic/larval fat body | third instar larval stage phenotype of Dhpr^HMC05508, Scer\GAL4^Cg.PA

(Zhou et al., 2019)

Marf^JF01650, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Marf^JF01650, Scer\GAL4^Bx-MS1096 is an enhancer of wing | male limited phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Marf^JF01650, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, egr^UAS.cIa

(Yang et al., 2013)

Marf^JF01650, Scer\GAL4^hs.2sev is an enhancer of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Suppressor of

Statement

Reference

Scer\GAL4^e33C/Marf^JF01650 is a suppressor | partially of mitochondrion | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Marf^JF01650 is a suppressor | partially of prohemocyte | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Scer\GAL4^e33C/Marf^JF01650 is a suppressor | partially of embryonic/larval lymph gland | third instar larval stage phenotype of asrij⁹

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is a suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is a suppressor of prohemocyte | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^dome-PG14 is a suppressor of embryonic/larval lymph gland | third instar larval stage phenotype of Scer\GAL4^dome-PG14, asrij^GD2167

(Ray et al., 2021)

Marf^JF01650, Scer\GAL4^elav.PU is a suppressor of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Marf^JF01650, Scer\GAL4^elav.PU is a suppressor of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^hs.2sev, BacA\p35^GMR.PH/Marf^JF01650 is a non-suppressor of eye phenotype of Mmus\Gria1^Lc.UAS, Scer\GAL4^hs.2sev

(Yang et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Miga^{Scer\UAS.T:Disc\RFP} partially suppresses mitochondrial defects seen in fat body cells in flies with expression of Marf^JF01650 driven by Scer\GAL4^Cg.PA.

(Zhang et al., 2016)

Expression of Marf^JF01650 under the control of Scer\GAL4^Bx-MS1096, leads to a clear enhancement of the wing phenotype found in flies expressing fog^Scer\UAS.cRa, with complete penetrance: in males, the wings appear small or stub-like, similar to parental males, and all females show short and abnormal wings, indicating that inhibiting mitochondrial fusion through down-regulation of Marf potentiates fog signalling.

(Ratnaparkhi, 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\UCP2^Scer\UAS.cFa and Marf^JF01650 under the control of Scer\GAL4^GMR.PU does not result in a rough eye phenotype.

(Hwang et al., 2014)

The presence of Marf^JF01650 strongly enhances the reduced adult eye size phenotype found in Scer\GAL4^hs.2sev->Mmus\Gria1^Lc.Scer\UAS flies.

A BacA\p35^GMR.PH/+ background fails to suppress the adult eye size defect found upon co-expression of Mmus\Gria1^Lc.Scer\UAS and Marf^JF01650 under the control of Scer\GAL4^hs.2sev.

(Yang et al., 2013)

Expression of Marf^JF01650 significantly suppresses the increase in mitochondria length seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU. The increases in neurotoxicity and cell cycle activation are also significantly rescued.

Expression of Marf^JF01650 suppresses the increase in superoxide production seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU.

(Duboff et al., 2012)

Complementation and Rescue Data

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Mutant

Wild-type

Stocks (1)

Bloomington

31157

y¹ v¹; P{TRiP.JF01650}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Marf^JF01650

Name Synonyms

Secondary FlyBase IDs

References (15)

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