FlyBase Allele Report: Dmel\CycG[RNAi.UAS]

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 increases total development time by 24 hours at 25[o]C. Mean cell size in the wing is increased in adult escapers.

44% of adult male escapers expressing CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 have over-rotated genitalia.

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PFa does not result in an eye defect.

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PH results in a weak increase in the number of ommatidia that is significant only in females. The ommatidia of mutant males are significantly larger in size than their control siblings.

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E does not change the posterior compartment area.

(Faradji et al., 2011)

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act.NP3121 results in complete male lethality at the late third instar larval stage 86% of females die, mostly as late pharates. Expression under the control of Scer\GAL4^da.G32 also results in a high percentage of lethality: 56% in males and 31% in females. Emerging flies show defects in the abdominal cuticle restricted to the posterior tergites A4 to A6. Abdominal bristles are disoriented and the tergites exhibit unsclerotised patches of variable size. Under the control of Scer\GAL4^da.G32 males are more strongly affected than females and also frequently exhibit rotated genitalia.

Unlike in wild type flies, the anterior and posterior dorsal histoblast nests of segment A4 to A6 remain small and unfused when CycG^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^Act.NP3121. The development of these flies is not delayed.

(Salvaing et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^da.G32/CycG^RNAi.UAS is an enhancer of homeotic | dominant phenotype of Abd-B^Fab7-1

(Salvaing et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 4 phenotype, enhanceable | male limited by corto⁴²⁰

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 5 phenotype, enhanceable | male limited by corto⁴²⁰

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 6 phenotype, enhanceable | male limited by corto⁴²⁰

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has male genitalia phenotype, enhanceable by corto⁴²⁰

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 4 phenotype, enhanceable by corto^07128b

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 5 phenotype, enhanceable by corto^07128b

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 6 phenotype, enhanceable by corto^07128b

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has male genitalia phenotype, enhanceable by corto^07128b

(Salvaing et al., 2008)

NOT Enhanced by

Statement

Reference

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 6 | female limited phenotype, non-enhanceable by corto^07128b/corto[+]

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 4 | female limited phenotype, non-enhanceable by corto^07128b/corto[+]

(Salvaing et al., 2008)

CycG^RNAi.UAS, Scer\GAL4^da.G32 has abdominal tergite 5 | female limited phenotype, non-enhanceable by corto^07128b/corto[+]

(Salvaing et al., 2008)

Enhancer of

Statement

Reference

Scer\GAL4^da.G32/CycG^RNAi.UAS is an enhancer of adult abdominal segment 6 phenotype of Abd-B^Fab7-1

(Salvaing et al., 2008)

Scer\GAL4^da.G32/CycG^RNAi.UAS is an enhancer of adult abdominal segment 7 | ectopic phenotype of Abd-B^Fab7-1

(Salvaing et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

corto⁴²⁰ enhances the frequency of cuticle defects and rotated genitalia seen in males when CycG^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^da.G32. No enhancement of the female cuticle defects is seen.

corto^07128b enhances the frequency of cuticle defects and rotated genitalia seen in males when CycG^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^da.G32. The frequency of female cuticle defects is also increased. The cuticle defects seen in females expressing CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act.NP3121 are not enhanced in the presence of one copy of corto^07128b.

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 slightly enhances the homeotic transformation from A7 to A6 seen in Abd-B^Fab7-1 male heterozygotes. The expressivity is slightly enhanced in 86% of flies but at least a thin A6 segment always persists. No cuticular defects are seen in either abdominal segment A5 or the remaining A6 tergites.

Expression of CycG^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Act.NP3121 and one copy of corto^07128b enhance the A7 to A6 homeotic transformation seen in the few Abd-B^Fab7-1/+ male escapers.

(Salvaing et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CycG^RNAi.UAS is rescued by Scer\GAL4^da.G32/CycG^UAS.cSa

(Salvaing et al., 2008)

CycG^RNAi.UAS is rescued by Scer\GAL4^Act.PU/CycG^UAS.cSa

(Salvaing et al., 2008)

Comments

Co-expression of CycG^Scer\UAS.cSa suppresses the lethality caused by expression of CycG^{dsRNA.Scer\UAS} under the control of either Scer\GAL4^da.G32 or Scer\GAL4^Act.

(Salvaing et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycG^RNAi.UAS

CycG^{dsRNA.Scer\UAS}

CycG^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

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