Nucleotide substitution: G2007A.
Amino acid replacement: W462term.
G16085069A
G2007A
W462term | Dg-PA; W379term | Dg-PB; W644term | Dg-PC; W727term | Dg-PD
W462term
TGG to TGA nonsense mutation in codon W462. Reported coordinates appear to be relative to GB:GH09323.
The Dg[-] progeny of DgO38/DgO86 females mated to DgO43/+ males are viable when grown without competition from heterozygous larvae.
The Dg[-] progeny of DgO43/DgO86 females mated to DgO55/+ males are viable when grown without competition from heterozygous larvae.
DgO86/DgO43 animals show reduced viability under normal culture conditions.
67% of eggs laid by DgO43/DgO86 females mated to wild-type males hatch.
85% of eggs laid by DgO38/DgO86 females mated to DgO43/+ males hatch.
79% of eggs laid by DgO43/DgO86 females mated to DgO55/+ males hatch.
Females carrying homozygous germline clones produce eggs that are normal in shape.
DgO43/DgO86 follicle cells show a defect in the organisation of the actin stress fibres; the fibres are still properly aligned in mutant follicle cells, but they do not align along the dorsal-ventral axis, and they sometimes show a mixed orientation within a single cell.
Under normal food conditions, DgO43 mutant follicle cell clones have normal apical-basal polarity.
Homozygotes show a posterior crossvein phenotype with 100% penetrance. More than 90% of wings show a "detached" phenotype (the crossvein is detached from both L4 and L5). A small fraction of wings with a "gapped" phenotype (the crossvein is detached from either vein L4 or L5, but not both) are seen.
DgO43/Df(2R)ED2457 animals show 63% viability compared to controls.