FB2024_03 , released June 25, 2024
Allele: Dmel\wun2αTub84B.Tag:MYC
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General Information
Symbol
Dmel\wun2αTub84B.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0212954
Feature type
allele
Associated gene
Dmel\wun2
Associated Insertion(s)
Carried in Construct
P{αTub84B(-FRT)wun2.myc}
Key Links
Transgenic product class
wild_type
(Renault et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Renault et al., 2004)
Mutagen
FLPase
(Renault et al., 2004)
Progenitor genotype
wun2FRT.αTub84B.Tag:MYC
(Renault et al., 2004)
Carried in construct
P{αTub84B(-FRT)wun2.myc}
Cytology
Description

The FRT cassette has been excised, juxtaposing the αTub84B promoter with the wun2 open reading frame (tagged with Tag:MYC).

(Renault et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
wun2
Tagged with
Tag:MYC
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Zygotic expression of wun2alphaTub84B.T:Hsap\MYC causes 6% of embryos to degenerate.

      (Steinhauer et al., 2009)

      Late stage embryos expressing wun2αTub84B.T:Hsap\MYC in the soma show a reduction in germ cell number compared to wild-type embryos.

      (Renault et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Enhancer of
      Statement
      Reference

      wun2αTub84B.Tag:MYC is an enhancer of embryo phenotype of Scer\GAL4Tub.PU, nesEY22898

      (Steinhauer et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Maternal overexpression of both nesEY22898 and wun2alphaTub84B.T:Hsap\MYC under the control of Scer\GAL4tub causes both a quantitative and a qualitative enhancement of the nesEY22898 embryo degeneration phenotype.

      (Steinhauer et al., 2009)

      Expression of wunScer\UAS.cZa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      (Renault et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of Mmus\Ppap2aScer\UAS.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells does not suppress the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      Expression of Hsap\PPAP2BScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      (Renault et al., 2004)
      Complementation and Rescue Data
      Partially rescued by

      wun2αTub84B.Tag:MYC is partially rescued by Scer\GAL4VP16.nanos.UTR/wun2UAS.Tag:MYC

      (Renault et al., 2004)

      wun2αTub84B.Tag:MYC is partially rescued by Scer\GAL4VP16.nanos.UTR/wun2Y225W.UAS.Tag:MYC

      (Renault et al., 2004)
      Not rescued by

      wun2αTub84B.Tag:MYC is not rescued by wun2H326K.UAS.Tag:MYC/Scer\GAL4VP16.nanos.UTR

      (Renault et al., 2004)
      Comments

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      Expression of wun2Y225W.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells does not suppress the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      (Renault et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wun2αTub84B.T:Hsap\MYC
      wun2αTub84B.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (4)