UASt regulatory sequences drive expression of Mmus\Plpp1 tagged at the C-terminal end with GFP.
Embryos expressing Mmus\Ppap2aScer\UAS.T:Avic\GFP under the control of Scer\GAL4twi.PG show no apparent perturbation or loss of primordial germ cells.
Scer\GAL4VP16.nanos.UTR/Mmus\Plpp1UAS.GFP is a non-suppressor of germline cell | maternal effect phenotype of wun2EP2650ex34/Df(2R)wun-GL
Scer\GAL4VP16.nanos.UTR/Mmus\Plpp1UAS.GFP is a non-suppressor of germline cell phenotype of wun2αTub84B.Tag:MYC
The germ cell death seen in embryos with a wun2+ soma which are derived from wun2EP2650ex34/Df(2R)wun-GL females is not rescued by expression of Mmus\Ppap2aScer\UAS.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.
Expression of Mmus\Ppap2aScer\UAS.T:Avic\GFP under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells does not suppress the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.
Carried in plasmid "Mouse Lpp1-GFP", transfected into S2 cells and used to study the catalytic activity of the protein produced.