FB2024_03 , released June 25, 2024
Allele: Dmel\wun2UAS.Tag:MYC
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General Information
Symbol
Dmel\wun2UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0122866
Feature type
allele
Associated gene
Dmel\wun2
Associated Insertion(s)
Carried in Construct
P{UAS-wun2.Myc}
Also Known As
UAS-wun2-myc, UAS wun2myc
Key Links
Transgenic product class
cDNA
(Starz-Gaiano et al., 2001)
wild_type
(Starz-Gaiano et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Starz-Gaiano et al., 2001)
wild_type
(Starz-Gaiano et al., 2001)
Mutagen
in vitro construct
(Starz-Gaiano et al., 2001)
Progenitor genotype
Carried in construct
P{UAS-wun2.Myc}
Cytology
Description

UASt regulatory sequences drive expression of wun2 tagged with Tag:MYC. β-globin 5' UTR sequences have also been added to the 5' end of the wun2 cDNA to enhance message stability and translation.

(Starz-Gaiano et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
wun2
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos expressing wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32 have reduced numbers of germ cells.

      (Ile et al., 2012)

      Expression of wun2Scer\UAS.T:Hsap\MYC in somatic cells under the control of Scer\GAL4twi.PG results in germ cell death in stage 15/16 embryos.

      (Renault et al., 2010)

      Expression of wun2Scer\UAS.T:Hsap\MYC in the primordial germ cells (under the control of Scer\GAL4nos.PG) does not increase apoptosis.

      (Sano et al., 2005)

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4twi.PG results in a reduction in the number of germ cells in the embryo.

      (Renault et al., 2004)

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B in embryos results in a germ cell migration phenotype. Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in embryos results in a germ cell migration phenotype.

      (Starz-Gaiano et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4btl.PS completely rescues the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of either Scer\GAL4how-24B or Scer\GAL4drm.7.1 fails to rescue the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.

      Very few animals which lack both maternal and zygotic wun and wun2 function (derived from females containing double mutant wun49 wun2EP2650ex34 germline clones mated to Df(2R)wun-GL/+ or Ab(2;?)wunCE/+ males) survive to adulthood. This lethality is only partially rescued by expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4btl.PS.

      (Ile et al., 2012)

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 rescues the germ cell death in embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones and wild type males.

      Homozygous srp2 suppresses the germ cell death seen at embryonic stage 15/16 when wun2Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4twi.PG.

      (Renault et al., 2010)

      Expression of wun2Scer\UAS.T:Hsap\MYC in the central nervous system, under the control of Scer\GAL49-201av restores bilateral sorting of primordial germ cells in a Df(2R)wun-GL (lacking both wun and wun2) background. Rescue is not complete however, as not all primordial germ cells reach a gonad.

      (Sano et al., 2005)

      Homozygosity for Df(3L)H99 does not suppress the reduction in germ cell number seen in embryos expressing wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4twi.PG.

      (Renault et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4VP16.nanos.UTR/wun2UAS.Tag:MYC rescues wun2EP2650ex34/Df(2R)wun-GL

      (Renault et al., 2004)
      Partially rescues

      Scer\GAL4VP16.nanos.UTR/wun2UAS.Tag:MYC partially rescues wun2αTub84B.Tag:MYC

      (Renault et al., 2004)
      Comments

      The germ cell death seen in embryos with a wun2+ soma which are derived from wun2EP2650ex34/Df(2R)wun-GL females is rescued by expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.

      Expression of wun2Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells partially suppresses the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      (Renault et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wun2Scer\UAS.T:Hsap\MYC
      wun2UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (10)