FB2024_03 , released June 25, 2024
Allele: Dmel\wun2H326K.UAS.Tag:MYC
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General Information
Symbol
Dmel\wun2H326K.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0122867
Feature type
allele
Associated gene
Dmel\wun2
Associated Insertion(s)
Carried in Construct
P{UAS-wun2.H326K}
Key Links
Transgenic product class
missense_variant
(Starz-Gaiano et al., 2001)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Starz-Gaiano et al., 2001)
Mutagen
in vitro construct
(Starz-Gaiano et al., 2001)
Progenitor genotype
wun2UAS.Tag:MYC
(Starz-Gaiano et al., 2001)
Carried in construct
P{UAS-wun2.H326K}
Cytology
Description

Construct: Amino acid replacement: H326K.

(Starz-Gaiano et al., 2001)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
wun2
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of both Scer\GAL4twi.PG and Scer\GAL4how-24B in embryos does not result in a germ cell migration defect (the germ cells enter the mesoderm as normal) and the number of germ cells is normal.

      (Starz-Gaiano et al., 2001)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4btl.PS fails to rescue the tracheal defects that are seen in embryos lacking both maternal and zygotic wun and wun2 function.

      (Ile et al., 2012)

      Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 is unable to rescue the germ cell death seen in embryos derived from females carrying homozygous wun49 wun2EP2650ex34 germline clones and wild type males.

      (Renault et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      wun2H326K.UAS.Tag:MYC/Scer\GAL4VP16.nanos.UTR fails to rescue wun2EP2650ex34/Df(2R)wun-GL

      (Renault et al., 2004)

      wun2H326K.UAS.Tag:MYC/Scer\GAL4VP16.nanos.UTR fails to rescue wun2αTub84B.Tag:MYC

      (Renault et al., 2004)
      Comments

      The germ cell death seen in embryos with a wun2+ soma which are derived from wun2EP2650ex34/Df(2R)wun-GL females is not rescued by expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16.

      Expression of wun2H326K.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the germ cells does not suppress the reduction in germ cell number seen in embryos expressing wun2αTub84B.T:Hsap\MYC in the soma.

      (Renault et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      wun2H326K.Scer\UAS.T:Hsap\MYC
      wun2H326K.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (5)