FlyBase Allele Report: Dmel\para[GD3392]

General Information

Symbol

Dmel\para^GD3392

Species

D. melanogaster

Name

FlyBase ID

FBal0210297

Feature type

allele

Associated gene

Dmel\para

Associated Insertion(s)

Carried in Construct

P{GD3392}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3392}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3392

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^Shu

(Kaas et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eclosion, with Scer\GAL4^elav.PU

(Kaas et al., 2016)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

lethal, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

lethal, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

lethal, with Scer\GAL4^elav-C155

(Parker et al., 2011)

paralytic | heat sensitive, with Scer\GAL4^insc-Mz1407

(Parker et al., 2011)

uncoordinated, with Scer\GAL4^109(2)80

(Tsubouchi et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

eye, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

terminal bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

Detailed Description

Statement

Reference

The expression of para^GD3392 under the control of Scer\GAL4^elav.PU alone leads to a significant decrease in climbing distance in negative geotaxis assays as compared to controls, whereas expression under the combined control of Scer\GAL4^elav.PU and Dicer-2 transgene (to enhance RNAi efficiency), results in lethality before adulthood.

results in defects at the neuromuscular junction of third instar larval abdominal muscles 6 and 7: expression under the control of Scer\GAL4^elav.PU alone results in a strong increase in the number of synaptic boutons of >=5micron diameter, despite a small but significant decrease in the total number of boutons, a strong increase in the number of 'ghost' boutons and a small but significant decrease in the number of branches, as compared to controls; expression under the control of Scer\GAL4^elav.PU results in a significant decrease in the frequency, but not amplitude, of miniature excitatory junction potentials and results in a significant increase in the latency period to stimuli, but not in the amplitude of corresponding excitatory junction potentials, as compared to controls.

(Xiao et al., 2017)

Expressing para^GD3392 under the control of Scer\GAL4^GMR.PU leads to a subtle rough eye phenotype.

(Iyer et al., 2016)

Individuals expressing para^GD3392 under the control of Scer\GAL4^elav.PU exhibit delayed eclosion

(Kaas et al., 2016)

Expression of para^GD3392 under the control of Scer\GAL4^elav.PLu decreases the penetrance of the bang sensitive phenotype seen in para^bss1/Y males ((from 100% to 65%) and para^bss1/+ females (from 87% to 1.6%).

Expression of two copies of para^GD3392 under the control of Scer\GAL4^elav-C155 results in complete lethality.

Expression of two copies of para^GD3392 under the control of Scer\GAL4^insc-Mz1407 results in flies being reversibly paralysed at 38[o]C.

(Parker et al., 2011)

Adults expressing para^GD3392 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3392

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, para^GD3392 has abnormal locomotor behavior | adult stage phenotype, enhanceable | female limited by Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, para^GD3392 has abnormal neurophysiology | third instar larval stage phenotype, non-enhanceable by Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav.PU/para^GD3392 is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of Cnx99A^KK108301

(Xiao et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/para^GD3392 is a suppressor | partially of abnormal neurophysiology | third instar larval stage phenotype of Cnx99A^KK108301

(Xiao et al., 2017)

para^GD3392, Scer\GAL4^ppk.1.9 is a suppressor of abnormal stress response phenotype of Crei\ChR2^UAS.cHa.EYFP, Scer\GAL4^ppk.1.9

(Stewart et al., 2012)

Other

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU, para^GD3392 has lethal | male limited phenotype

(Xiao et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, para^GD3392 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, para^GD3392 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Scer\GAL4^elav.PU, para^GD3392 has terminal bouton | third instar larval stage phenotype, suppressible | partially by Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

NOT Enhancer of

Statement

Reference

para^GD3392, Scer\GAL4^elav.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Suppressor of

Statement

Reference

para^GD3392, Scer\GAL4^elav.PU is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

para^GD3392, Scer\GAL4^elav.PU is a suppressor | partially of terminal bouton | third instar larval stage phenotype of Cnx99A^KK108301, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of para^GD3392 and Cnx99A^KK108301 under the control of Scer\GAL4^elav.PU alone leads to male lethality and enhances the locomotor defects in negative geotaxis assays exhibited by females expressing para^GD3392 alone.

At the neuromuscular junction of third instar larval abdominal muscles 6 and 7, the co-expression of para^GD3392 and Cnx99A^KK108301 under the control of Scer\GAL4^elav.PU partially suppresses the increased total number of boutons induced by the expression of Cnx99A^KK108301 alone, and suppresses the increased 'ghost' bouton number, but not the increase in the number of synaptic boutons of >=5micron diameter, the decrease in the total number of boutons or the decrease in the number of branches, induced by the expression of para^GD3392 alone; the co-expression of para^GD3392 and Cnx99A^KK108301 under the control of Scer\GAL4^elav.PU does not significantly enhance the increased latency period to stimuli induced by the expression of either Cnx99A^KK108301 alone or para^GD3392 alone, but partially suppresses the decreased miniature excitatory junction potentials induced by the expression of either Cnx99A^KK108301 alone.

(Xiao et al., 2017)

Xenogenetic Interactions

Statement

Reference

Crei\ChR2^{Scer\UAS.cHa.T:Avic\GFP-EYFP} larvae expressing para^GD3392 under the control of Scer\GAL4^ppk.1.9 exhibit a reduction in nocifensive escape locomotion in response to blue-light stimulation.

(Stewart et al., 2012)

Complementation and Rescue Data

Comments

The expression of para^GD3392 under the control of Scer\GAL4^elav.PU, but not of Scer\GAL4^Mhc.PU, under 25[o]C temperature, leads to strong decreases in wing posture defects and in the movement time spent in the centre of a circular space exhibited by para^Shu heterozygous females.

(Kaas et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v6131

w¹¹¹⁸; P{GD3392}v6131

v6132

w¹¹¹⁸; P{GD3392}v6132/TM3

Notes on Origin

Discoverer