Larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP in class IV nociceptors under the control of Scer\GAL4ppk.1.9 exhibit robust nocifensive escape locomotion in response to blue-light illumination in 69% of animals.
Larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL41003.3 and fed yeast paste containing all-trans retinal show gentle touch-like behavioural responses upon blue light illumination.
Expression of Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL4tutl-GAL4 in the presence of all-trans retinal does not produce touch-like behavioural responses upon blue light illumination.
Third instar larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL4109(2)80 and fed yeast paste containing all-trans retinal (the chromophore for Crei\ChR2) show a behavioural response to blue light pulses: the larvae simultaneously contract the muscles of all body segments and scrunch up like a compressed accordion. Rarely, the larvae first roll with a motor pattern that appears to be quite similar to nocifensive rolling behaviour before undergoing the accordion-like muscle contraction.
Third instar larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of either Scer\GAL4smid-C161 or Scer\GAL41003.3 and fed yeast paste containing all-trans retinal show a high frequency of accordion-like muscle contraction in response to blue light, but never show rolling behaviour.
Third instar larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL4ppk.1.9 and fed yeast paste containing all-trans retinal show a high frequency of rolling behaviour in response to blue light which is qualitatively similar to nocifensive behaviour (although the initiation of the response is more rapid) , but never show an accordion-like muscle contraction.
Third instar larvae expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL42-21 and fed yeast paste containing all-trans retinal do not show either accordion-like muscle contraction or rolling behaviour in response to blue light.
Crei\ChR2UAS.cHa.EYFP, Scer\GAL4ppk.1.9 has abnormal stress response phenotype, suppressible by paraGD3392, Scer\GAL4ppk.1.9
Crei\ChR2UAS.cHa.EYFP, Scer\GAL4ppk.1.9 has abnormal stress response phenotype, suppressible by spasKK107786, Scer\GAL4ppk.1.9
Crei\ChR2UAS.cHa.EYFP, Scer\GAL4ppk.1.9 has abnormal stress response phenotype, suppressible by spas[+]/spas5.75
Crei\ChR2UAS.cHa.EYFP, Scer\GAL4ppk.1.9 has abnormal stress response phenotype, non-suppressible by kat-60L1GD7435, Scer\GAL4ppk.1.9
Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP larvae expressing paraGD3392 under the control of Scer\GAL4ppk.1.9 exhibit a reduction in nocifensive escape locomotion in response to blue-light stimulation.
Nocifensive escape locomotion in response to blue-light stimulation is unaffected in Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP flies expressing kat-60L1GD7435 under the control of Scer\GAL4ppk.1.9.
Expression of spasKK107786 in class IV nociceptors (under the control of Scer\GAL4ppk.1.9) results in a reduction of the nocifensive escape locomotion response to blue-light stimulation in Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP expressing flies.
A spas5.75 heterozygous background results in a reduction of the nocifensive escape locomotion response to blue-light stimulation in flies expressing Crei\ChR2Scer\UAS.cHa.T:Avic\GFP-EYFP under the control of Scer\GAL4ppk.1.9.