FlyBase Allele Report: Dmel\kat-60L1[GD7435]

General Information

Symbol

Dmel\kat-60L1^GD7435

Species

D. melanogaster

Name

FlyBase ID

FBal0199623

Feature type

allele

Associated gene

Dmel\kat-60L1

Associated Insertion(s)

Carried in Construct

P{GD7435}

Also Known As

UAS-Katanin-p60L1-RNAi, kat-60L1 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD7435}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD7435

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response, with Scer\GAL4^ppk.1.9

(Stewart et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

abnormal neuroanatomy | P-stage, with Scer\GAL4^ppk.PU

(Tao et al., 2016)

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal stress response, with Scer\GAL4^ppk.1.9

(Stewart et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

dendrite, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

dendrite & dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL4^ppk.PG

(Lee et al., 2009)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Tao and Rolls, 2011)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of kat-60L1^GD7435 under the control of Scer\GAL4^ppk.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not lead to any significant defects in axon or dendrite clearance after injury, but does lead to defective dendrite pruning 18h after the onset of pupariation, as compared to controls.

(Tao et al., 2016)

Larval ddaE neurons expressing kat-60L1^GD7435 under the control of Scer\GAL4²²¹ (and Dicer-2, for a more efficient RNAi) do not show significant changes in the number of growing microtubules within the cell body 24h after axotomy, as compared to similarly injured control neurons.

(Chen et al., 2012)

Expression of kat-60L1^GD7435 in class IV da neurons, under the control of Scer\GAL4^ppk.1.9 results in a reduction in mechanical nocifensive response.

Class IV neuron-specific RNAi knockdown of kat-60L1 through expression of kat-60L1^GD7435 under the control of Scer\GAL4^ppk.1.9 results in a severe delay in larval response to a high-temperature probe of 46[o]C. This insensitivity is maintained at a lower threshold temperature of 42[o]C, indicating an overall failure to respond to noxious temperatures.

(Stewart et al., 2012)

A reduction in the levels of kat-60L1 through expression of kat-60L1^GD7435 in ddaC dendrites under the control of Scer\GAL4^ppk.1.9 results in a strong reduction in the level of dendritic pruning. However, dendrite degeneration due to dendrite severing proceeds as in wild-type.

(Tao and Rolls, 2011)

Adults expressing kat-60L1^GD7435 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD7435} insertion line used.

(Neely et al., 2010)

The proximal dendrites remain intact for many ddaC neurons at 16 hours after puparium formation (APF) in animals expressing kat-60L1^GD7435 under the control of Scer\GAL4^ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).

(Lee et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7435

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-enhancer of abnormal neuroanatomy | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-enhancer of abnormal cell shape | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

NOT Suppressor of

Statement

Reference

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-suppressor of abnormal neuroanatomy | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-suppressor of abnormal cell shape | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

kat-60L1^GD7435, Scer\GAL4^ppk.1.9 is a non-suppressor of abnormal stress response phenotype of Crei\ChR2^UAS.cHa.EYFP, Scer\GAL4^ppk.1.9

(Stewart et al., 2012)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-enhancer of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-enhancer of dendrite | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

NOT Suppressor of

Statement

Reference

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-suppressor of larval dorsal multidendritic neuron ddaC | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

kat-60L1^GD7435, Scer\GAL4^ppk.PU is a non-suppressor of dendrite | pupal stage phenotype of Patronin^KK100898, Scer\GAL4^ppk.PU

(Wang et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Nocifensive escape locomotion in response to blue-light stimulation is unaffected in Crei\ChR2^{Scer\UAS.cHa.T:Avic\GFP-EYFP} flies expressing kat-60L1^GD7435 under the control of Scer\GAL4^ppk.1.9.

(Stewart et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v31598

w¹¹¹⁸; P{GD7435}v31598

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

7435

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG1193^GD7435

kat-60L1^GD7435

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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