FlyBase Allele Report: Dmel\Cnx99A[KK108301]

General Information

Symbol

Dmel\Cnx99A^KK108301

Species

D. melanogaster

Name

FlyBase ID

FBal0235485

Feature type

allele

Associated gene

Dmel\Cnx99A

Associated Insertion(s)

Carried in Construct

P{KK108301}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108301}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108301

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

terminal bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Xiao et al., 2017)

Detailed Description

Statement

Reference

The expression of Cnx99A^KK108301 under the combined control of Scer\GAL4^elav.PU and of the Dicer-2 transgene (to enhance RNAi efficiency), but not under the control of Scer\GAL4^elav.PU alone, leads to a significant decrease in locomotor capacity in negative geotaxis assays, as compared to controls.

The expression of Cnx99A^KK108301 results in defects at the neuromuscular junction of third instar larval abdominal muscles 6 and 7: expression under the control of Scer\GAL4^elav.PU alone results in a significant increase in the number of >=5micron synaptic boutons, despite an insignificant change in the total number of boutons, and insignificant changes in the number of 'ghost' boutons and in the number of branches, as compared to controls; expression under the control of Scer\GAL4^elav.PU results in a significant decrease in the frequency, but not amplitude, of miniature excitatory junction potentials and results in a significant increase in the latency period to stimuli, without affecting the amplitude of the corresponding excitatory junction potentials, as compared to controls.

(Xiao et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cnx99A^KK108301 has abnormal neurophysiology | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^elav.PU/para^GD3392

(Xiao et al., 2017)

Suppressed by

Statement

Reference

Cnx99A^KK108301 has abnormal neurophysiology | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^elav.PU/para^GD3392

(Xiao et al., 2017)

Enhancer of

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU is an enhancer | female limited of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^elav.PU, para^GD3392

(Xiao et al., 2017)

Scer\GAL4^sca.PU/Cnx99A^KK108301 is an enhancer of paralytic | adult stage | temperature conditional phenotype of para^ts1

(Xiao et al., 2017)

NOT Enhancer of

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^elav.PU, para^GD3392

(Xiao et al., 2017)

Other

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU, para^GD3392 has lethal | male limited phenotype

(Xiao et al., 2017)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by para^GD3392, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Suppressed by

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by para^GD3392, Scer\GAL4^elav.PU

(Xiao et al., 2017)

Cnx99A^KK108301, Scer\GAL4^elav.PU has terminal bouton | third instar larval stage phenotype, suppressible | partially by para^GD3392, Scer\GAL4^elav.PU

(Xiao et al., 2017)

NOT Enhancer of

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^elav.PU, para^GD3392

(Xiao et al., 2017)

Suppressor of

Statement

Reference

Cnx99A^KK108301, Scer\GAL4^elav.PU is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^elav.PU, para^GD3392

(Xiao et al., 2017)

Cnx99A^KK108301, Scer\GAL4^elav.PU is a suppressor | partially of terminal bouton | third instar larval stage phenotype of Scer\GAL4^elav.PU, para^GD3392

(Xiao et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Cnx99A^KK108301 under the control of Scer\GAL4^sca.PU significantly enhances the paralysis phenotype of para^ts1 homozygotes induced by temperature shift to 38[o]C, as shown by a slower recovery from paralysis upon return to room temperature.

At the neuromuscular junction of third instar larval abdominal muscles 6 and 7, the co-expression of Cnx99A^KK108301 and para^GD3392 under the control of Scer\GAL4^elav.PU partially suppresses the increased total number of boutons induced by the expression of Cnx99A^KK108301 alone, and suppresses the increased 'ghost' bouton number, but not the increase in the number of synaptic boutons of >=5micron diameter, the decrease in the total number of boutons or the decrease in the number of branches, induced by the expression of para^GD3392 alone; the co-expression of Cnx99A^KK108301 and para^GD3392 under the control of Scer\GAL4^elav.PU does not significantly enhance the increased latency period to stimuli induced by the expression of either Cnx99A^KK108301 alone or para^GD3392 alone, but partially suppresses the decreased miniature excitatory junction potentials induced by the expression of either Cnx99A^KK108301 alone.

(Xiao et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Cnx99A^KK108301 is partially rescued by Scer\GAL4^elav.PU/Cnx99A^UAS.Tag:FLAG

(Xiao et al., 2017)

Comments

The adult locomotion defects resulting from the expression of Cnx99A^KK108301 under the combined control of Scer\GAL4^elav.PU and Dicer-2 transgene are partially rescued by the co-expression of Cnx99A^{Scer\UAS.T:Zzzz\FLAG}.

(Xiao et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v100740

P{KK108301}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cnx99A^KK108301

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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