FlyBase Allele Report: Dmel\Zip89B[GD3013]

General Information

Symbol

Dmel\Zip89B^GD3013

Species

D. melanogaster

Name

FlyBase ID

FBal0209026

Feature type

allele

Associated gene

Dmel\Zip89B

Associated Insertion(s)

Carried in Construct

P{GD3013}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3013}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3013

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

viable, with Scer\GAL4^Tub.PU

(Lye et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Zip89B^GD3013 under the control of Scer\GAL4^GMR.PFa does not result in a mutant phenotype.

Expression of Zip89B^GD3013 under the control of Scer\GAL4^pnr-MD237 does not result in a mutant phenotype.

(Lye et al., 2012)

Expression via P{GAL4-tim.E}27 or P{cry-GAL4.Z}17 (in the presence of Dcr-2^Scer\UAS.cDa) does not significantly affect circadian rhythmicity in locomotor activity assays in constant darkness.

(Mandilaras and Missirlis, 2012)

Adults expressing Zip89B^GD3013 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Zip89B^GD3013 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3013

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^pnr-MD237 is a non-enhancer of visible phenotype of Scer\GAL4^pnr-MD237, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

Suppressor of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^GMR.PFa is a suppressor of visible phenotype of Scer\GAL4^GMR.PFa, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

NOT Suppressor of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zip71B^UAS.Tag:FLAG

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.long, Zip71B^UAS.Tag:FLAG

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^pnr-MD237 is a non-suppressor of visible phenotype of Scer\GAL4^pnr-MD237, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^pnr-MD237 is a non-enhancer of adult thorax phenotype of Scer\GAL4^pnr-MD237, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

Suppressor of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^GMR.PFa is a suppressor of eye phenotype of Scer\GAL4^GMR.PFa, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

NOT Suppressor of

Statement

Reference

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, Zip71B^UAS.Tag:FLAG

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.cDa

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, ZnT86D^UAS.EGFP

(Dechen et al., 2015)

Zip89B^GD3013/Scer\GAL4^pnr-MD237 is a non-suppressor of adult thorax phenotype of Scer\GAL4^pnr-MD237, Zip42C.1^UAS.EGFP, ZnT63C^VDRC.cUa

(Lye et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86D^Scer\UAS.cDa under the control of Scer\GAL4^GMR.long do not change upon co-expression of Zip89B^GD3013 RNAi.

The morphological and pigmentation defects in the adult eye seen upon expression of ZnT86D^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.long do not change upon co-expression of Zip89B^GD3013 RNAi.

The morphological and pigmentation defects in the adult eye seen upon expression of Zip71B^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.long do not change upon co-expression of Zip89B^GD3013 RNAi.

(Dechen et al., 2015)

The mutant eye phenotype seen in flies co-expressing both ZnT63C^VDRC.cUa and Zip42C.1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PFa is suppressed if the flies are also co-expressing Zip89B^GD3013.

Co-expression of Zip89B^GD3013 has no effect on the mutant thorax phenotype seen in flies co-expressing both ZnT63C^VDRC.cUa and Zip42C.1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^pnr-MD237.

(Lye et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v37358

w¹¹¹⁸; P{GD3013}v37358

v37357

w¹¹¹⁸; P{GD3013}v37357/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

3013

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Zip3^GD3013

Zip89B^GD3013

dZip89B^RNAi

(Dechen et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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