FlyBase Allele Report: Dmel\cyc[01]

General Information

Symbol

Dmel\cyc⁰¹

Species

D. melanogaster

Name

FlyBase ID

FBal0195440

Feature type

allele

Associated gene

Dmel\cyc

Associated Insertion(s)

Carried in Construct

Also Known As

cyc⁰

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Rutila et al., 1998)

Progenitor genotype

Cytology

Description

Amino acid replacement: K159term.

(Rosbash, 2006.8.10, Rutila et al., 1998)

Has a nonsense mutation in the PAS-encoding region.

(Park et al., 2000)

Nucleotide substitution: A586T.

(Rutila et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:19,814,882..19,814,882 [-]

Nucleotide change:

A19814882T

Reported nucleotide change:

A586T

Amino acid change:

K159term | cyc-PA

Reported amino acid change:

K159term

(Park et al., 2000, Rutila et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart

(Gill et al., 2015)

LN period neuron

(Park et al., 2000)

Detailed Description

Statement

Reference

The 5-week old cyc⁰¹ mutant flies subjected to ad libitum feeding (ARF) have a slower heart rate compared to controls. The heart period does not change significantly on 12-hour daytime-restricted feeding (TRF). TRF increases arrhythmia in cyc⁰¹ mutant flies.

(Gill et al., 2015)

cyc⁰¹/cyc⁰¹ flies lose circadian rhythms (0% show rhythmicity) under dark conditions.

(Liu et al., 2015)

Sleep bout duration and total daily sleep are significantly reduced compared to controls in mutant flies, while sleep latency is significantly increased.

(Liu et al., 2014)

1 day memory retention is normal after spaced training in an olfactory conditioning assay.

(Chen et al., 2012)

Triglyceride levels in cyc⁰¹ mutants are similar to wild-type.

(Diangelo et al., 2011)

Naive and trained responses to a magnetic field are not impaired in cyc⁰¹ mutant flies.

(Gegear et al., 2010)

cyc⁰¹ mutants do not display phenotypes related to either survival time under starvation conditions or glycogen storage. Total food consumption is also unchanged in these mutants compared to controls.

(Xu et al., 2008)

The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc⁰¹.

(Fujii et al., 2007)

cyc⁰¹ has no effect on experience-dependent responses in sleep need.

(Ganguly-Fitzgerald et al., 2006)

With 5-HTP treatment, daily sleep and night-time sleep bout length are increase in cyc⁰¹ mutant flies, compared to controls where daily sleep is increased but there is no change in night-time sleep bout length.

(Yuan et al., 2006)

cyc⁰¹ mutant larvae are highly sensitive to light (shown in light avoidance assays), even at low intensities (150 lux).

cyc⁰¹ larvae entrained in LD cycles for 2-3 days and then shifted into DD, that are tested for their ability to avoid light at 3hr intervals on the second day of DD do not show a detectable rhythm, appearing constitutively sensitive.

(Mazzoni et al., 2005)

There is no significant increase in the time spent copulating by pairs of cyc⁰¹ homozygous flies compared to wild-type pairs.

(Beaver and Giebultowicz, 2004)

Long term memory of courtship conditioning (reduction in time spent in courtship behaviour 5 days after a 7 hour conditioning) is normal in cyc⁰ homozygous males.

(Sakai et al., 2004)

The diurnal changes in the mean electroantennogram (EAG) response to ethyl acetate which are seen in wild-type flies maintained in 12 hour light:12 hour dark cycles are abolished in cyc⁰¹ flies.

(Tanoue et al., 2004)

Circadian locomotor rhythms are also absent in cyc⁰¹/cyc⁰¹ flies.

(Peng et al., 2003)

cyc⁰¹ mutant females mated to cyc⁰¹ mutant males lay significantly less eggs and produce significantly less progeny than wild-type flies. The percentage of unfertilised eggs from this mating is not significantly greater than for wild-type.

(Beaver et al., 2002)

Mutant females show a disproportionately large sleep rebound after sleep deprivation (in constant darkness). Increasing the length of sleep deprivation to more than 3 hours results in the flies showing large increases in sleep that persists as long as the flies are recorded (up to 16 days). A second period of sleep deprivation results in further increases in sleep. The flies begin to die if deprived of sleep for more than 10 hours (mortality is 33 +/- 4% for 12 hours of sleep deprivation). Mutant flies that are deprived of sleep for 30 minutes each hour for 24 hours do not die, indicating that it is not the stimulus used for sleep deprivation that causes the flies to die after 12 hours of constant sleep deprivation. The genetic background and age of the flies does not influence the sleep phenotype. Mutant males recover 100% of lost sleep and die after 12 hours of sleep deprivation. Exposure of mutant females to heat before 12 hours of sleep deprivation protects them from the lethal effects of sleep deprivation and reduces homeostatic drive (sleep rebound). cyc⁰¹/Df(3L)kto2 flies also show an exaggerated homeostatic response (sleep rebound) and deaths after 12 hours of sleep deprivation.

(Shaw et al., 2002)

About a quarter of cyc⁰ adult brains exhibit an abnormal dorsal projection from the l-LN_v cells. In about 30% of these mutant specimens the projections are asymmetric within a single individual: one hemisphere might contain a bundle of dorsally projected axons; but in the contralateral hemisphere, only one or two axons might project into the dorsal brain. In other cyc⁰ adults, axons stemming from the region project irregularly into a median brain region.

(Park et al., 2000)

Homozygotes are arrhythmic with respect to locomotor activity and adult eclosion under constant darkness conditions. This phenotype is not altered if the flies are also mutant for either per^L1 or per^s. Only 64% of homozygotes have discernible locomotor rhythms under 12 hour light:12 hour dark conditions. Heterozygotes have robust locomotor rhythms with no hint of arrhythmicity, but they have altered periods with rhythms 1 hour longer than normal. Homozygotes behave normally in an optomotor behaviour test.

(Rutila et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Gprk2^Scer\UAS.cTa under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} induces a robust electroantennogram response in a cyc⁰¹ background.

(Tanoue et al., 2008)

Expression of Clk^DN.Scer\UAS in the fat body, under the control of Scer\GAL4^to.PD in a cyc⁰¹ background fails to affect starvation sensitivity. Glycogen levesl are lower than those of cyc⁰¹ flies, but the decrease is less than that seen in heterozygous cyc⁰¹ flies with a disrupted fat body clock.

(Xu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

cyc⁰²

(Park et al., 2000)

Rescued by

cyc⁰¹ is rescued by cyc^7.2.Tag:FLAG

(Abruzzi et al., 2011)

cyc⁰¹ is rescued by Scer\GAL4^Or22a.8197/cyc^UAS.Tag:MYC

(Tanoue et al., 2004)

cyc⁰¹ is rescued by Scer\GAL4^{Orco.2.642.Hsim\VP22}/cyc^UAS.Tag:MYC

(Tanoue et al., 2004)

Partially rescued by

cyc⁰¹ is partially rescued by cyc^EGFP

(Liu et al., 2015)

cyc⁰¹ is partially rescued by cyc^UAS.Tag:HA/Scer\GAL4^P2.4.Pdf

(Peng et al., 2003)

Not rescued by

cyc⁰¹ is not rescued by Scer\GAL4^{Orco.2.642.Hsim\VP22}/cyc^UAS.Tag:MYC

(Fujii et al., 2007)

Comments

cyc^{T:Avic\GFP-EGFP} significantly rescues circadian rhythms (84% of flies show rhythmicity with a 25.9hr period) in cyc⁰¹/cyc⁰¹ flies under dark conditions.

(Liu et al., 2015)

The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc⁰¹. This phenotype is not rescued by cyc^{Scer\UAS.T:Hsap\MYC}; Scer\GAL4^{Or83b.2.642.T:Hsim\VP22}.

(Fujii et al., 2007)

cyc⁰¹ and cyc⁰² are non complementing both for behaviour and for a severe reduction in levels of per protein.

(Park et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80929

cyc⁰¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cyc⁰

(Glossop et al., 1999)

cyc01

(Rosbash, 2006.8.10)

cyc⁰

(Parasram et al., 2018, Chen et al., 2012, Ruben et al., 2012, Blanchard et al., 2010, Gegear et al., 2010, Rosbash, 2006.8.10, Yuan et al., 2006, Mazzoni et al., 2005, Yuan et al., 2005, Yuan et al., 2005, Park et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBal0105034
FBal0090449

References (64)

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