In Src64B^ko/+ wandering third instar larvae CadN-immunopositive adherens junctions between photoreceptor precursor cells R3 and R4 in row 5 have similar lengths as wild-type controls.

Src64B^ko/+ does not significantly alter baseline neurotransmission at the NMJ of third instar larvae.

Src64B^ko mutant nephrocytes exhibit morphological changes, the most significant of which is a shortening and reduction in numbers of labyrinthine channels with the consequent displacement of vacuoles and organelles towards the periphery of the cell and nephrocyte agglutination. Regions are seen that are practically devoid of filtration diaphragms and more than one diaphragm can be seen closing the entrance of the labyrinthine channels. The formation of ectopic diaphragms is observed at internal protrusions from the membrane, and this is never observed in wild type. Electron-dense material, reminiscent of adherens junctions, is seen at the membrane in regions of nephrocyte apposition.

Homozygous females lay significantly fewer eggs than wild type. 15% of these eggs hatch.

Src64B^D404N/Src64B^ko females lay significantly fewer eggs than wild type. 28% of these eggs hatch.

Src64B^Δ17/Src64B^ko females lay significantly fewer eggs than wild type. 43% of these eggs hatch.

Eggs laid by Src64B^P190L/Src64B^ko, Src64B^G208E/Src64B^ko, Src64B^R217C/Src64B^ko, Src64B^D372N/Src64B^ko or Src64B^R403C/Src64B^ko females hatch at a normal rate.

Ring canal diameter in homozygous egg chambers significantly smaller than wild type. Nurse cell fusion is seen in the mutant egg chambers.

Ring canal diameter in Src64B^D404N/Src64B^ko egg chambers significantly smaller than wild type.

Homozygous embryos derived from homozygous females show defects during cellularisation. The microfilament rings show significantly more deviation from circularity than in wild-type embryos during both early and late cellularisation.

Src64B^ko females display severe fertility defects; these include severely reduced egg lay and hatch rates.

The egg chambers of Src64B^ko females contain aberrantly small ring canals (on average 5.1μm diameter vs 9.1μm in wild-type females). Src64B^ko ring canals exhibit defects in attaching to the cortical membrane.

Src64B^ko egg chambers have aberrant cell numbers. Egg chambers with too many or too few germ cells are occasionally neighbors, and, on rare occasions, there are entirely fused ovarioles. The total number of germ cells is usually a multiple of the wild-type number of 16. In cases where adjacent egg chambers are abnormal, the sum of the two is uaually a multiple of 16 with a 15:1 ratio of nurse cells to egg chambers. For example, egg chambers have been observed with 22 nurse cells and one oocyte next to an egg chamber containing 8 nurse cells and 1 oocyte and egg chambers have also been observed with 24 nurse cells and 2 oocytes next to an egg chamber containing 6 nurse cells. The penetrance of the phenotype increases with age and temperature. Most Src64B^ko egg chambers exhibit severe ring canal attachment defects, including floating ring canals and cortical membrane collapse, making it difficult to accurately score ring canal number in defective egg chambers.

Oocyte specification occurs normally in most mispackaged Src64B^ko egg chambers; in most cases, oocyte absence is correlated with membrane collapse.

Src64B^ko germ cells are indistinguishable from wild-type in terms of size and localization of adhesion molecules in the germarium. However, in many cases nurse cell morphology is subsequently affected by detachment of ring canals from the cortical membrane as early as stage 1. Gaps in the follicle cell layer and follicle cell polarity defects are not observed and polar and stalk cell differentiation is normal in Src64B^ko ovarioles. Src64B^ko egg chambers are always separated by stalks, even when egg chambers are mispackaged.

In Src64B^ko germaria, cysts with aberrant cell numbers are frequently observed and, in rare cases, cells are separated from the rest of the cyst. The follicle cells in these germaria extend projections and migrate to fully surround the germline cysts. Germline cysts in Src64B^ko germaria often fail to flatten in region 2B, resulting in the simultaneous packaging of side-by-side cysts. There is a higher penetrance of packaging defects in germaria versus vitellaria.

Src64B^Δ17/Src64B^ko transheterozygous females show egg chamber packaging defects.

Src64B[+]/Src64B^ko is a suppressor | partially of abnormal neuroanatomy | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor | partially of abnormal cell adhesion | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor | partially of abnormal size | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of fra⁶/fra³

Src64B[+]/Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src64B^ko/Src64B^ko is a suppressor of visible phenotype of ASPP⁸

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has abnormal neurophysiology | third instar larval stage phenotype

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has abnormal neuroanatomy phenotype

Src64B^ko is an enhancer of fascicle phenotype of Src42A^k10108/Src42A^E1

Src64B^ko is an enhancer of symmetrical commissure phenotype of Src42A^k10108/Src42A^E1

Src64B^ko is a non-enhancer of fascicle phenotype of Src42A^E1

Src64B^ko is a non-enhancer of symmetrical commissure phenotype of Src42A^E1

Src64B[+]/Src64B^ko is a suppressor of embryo | maternal effect phenotype of Cul5^GM163/Cul5^GM130

Src64B[+]/Src64B^ko is a suppressor of nucleus | maternal effect | embryonic cycle 6 phenotype of Cul5^GM163/Cul5^GM130

Src64B[+]/Src64B^ko is a suppressor | partially of photoreceptor R3 precursor cell | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor | partially of photoreceptor R4 precursor cell | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor | partially of adherens junction | wandering third instar larval stage phenotype of mbt^P1

Src64B[+]/Src64B^ko is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src64B[+]/Src64B^ko is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B[+], Src64B^ko is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src42A^k10108, Src42A[+], Src64B^ko is a suppressor of symmetrical commissure phenotype of Scer\GAL4^eg-Mz360, fra^{ΔC.UAS.Tag:HA}

Src64B[+]/Src64B^ko is a suppressor of larval EW neuron phenotype of fra⁶/fra³

Src64B[+]/Src64B^ko is a suppressor of larval posterior commissure phenotype of fra⁶/fra³

Src64B[+]/Src64B^ko is a suppressor of larval longitudinal connective phenotype of fra⁶/fra³

Src64B^ko/Src64B^ko is a suppressor of eye phenotype of ASPP⁸

Src64B^ko is a non-suppressor of fascicle phenotype of Src42A^E1

Src64B^ko is a non-suppressor of symmetrical commissure phenotype of Src42A^E1

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has embryonic/larval neuromuscular junction | third instar larval stage phenotype

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has symmetrical commissure phenotype

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has fascicle phenotype