Clones generated in the larval eye discs expressing Csk^unspecified and Ras85D^V12.Scer\UAS show limited tissue overgrowth.

Expression of Nek2^{Scer\UAS.T:Avic\GFP} enhances the tissue overgrowth seen in larval eye disc clones expressing Csk^unspecified and Ras85D^V12.Scer\UAS, and leads to the production of secondary tumours in the body of the larvae. This phenotype can be suppressed by feeding flies with the Akt1 inhibitor MZ2206; significantly smaller primary tumours are seen and there is a complete absence of secondary tumours.

Injection of dissociated cells from Nek2^{Scer\UAS.T:Avic\GFP} Csk^unspecified Ras85D^V12.Scer\UAS mutant eye discs (generated using ey-FLP MARCM) into the dorsal notum region of wild type adult flies results in detection of T:Avic\GFP positive cells in various parts of the adult body. Dispersed Avic\GFP-positive cells are not seen when flies are injected with eye disc cells that express Nek2^{Scer\UAS.T:Avic\GFP} alone.

Mutation of Csk partially suppresses the ring canal size defects of Src64B^Δ17 ring canals. Csk^unspecified, Src64B^Δ17 double homozygotes show larger ring canals than Csk^unspecified/+, Src64B^Δ17 females.

The penetrance of the egg chamber packaging defects of Src64B^Δ17 females is significantly reduced by removal of one copy of Csk (genotype: Src64B^Δ17/Src64B^Δ17, Csk^unspecified/+). The penetrance of egg chamber packaging defects of Src64B^Δ17/Src64B^ko transheterozygotes is somewhat reduced in Src64B^Δ17/Src64B^ko, Csk^unspecified/+ females, while the penetrance of the phenotype is not significantly reduced in Src64B^ko females.