FlyBase Allele Report: Dmel\Src42A[E1]

General Information

Symbol

Dmel\Src42A^E1

Species

D. melanogaster

Name

FlyBase ID

FBal0103956

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tateno et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?483term.

(Tateno et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:6,011,505..6,011,505 [+]

Nucleotide change:

G6011505A

Reported nucleotide change:

G?A

Amino acid change:

W483term | Src42A-PA; W1563term | Src42A-PB

Reported amino acid change:

?483term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Tateno et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(O'Donnell and Bashaw, 2013)

abnormal wound healing | embryonic stage

(Campbell et al., 2021)

lethal - all die before end of first instar larval stage

(Matusek et al., 2006)

lethal - all die during embryonic stage | recessive

(Tateno et al., 2000)

some die during embryonic stage | recessive

(Tateno et al., 2000)

some die during first instar larval stage

(Matusek et al., 2006)

Phenotype Manifest In

cephalopharyngeal skeleton

(Tateno et al., 2000)

embryonic/larval tracheal section

(Matusek et al., 2006)

fascicle

(O'Donnell and Bashaw, 2013)

presumptive embryonic salivary gland

(Harris and Beckendorf, 2007)

symmetrical commissure

(O'Donnell and Bashaw, 2013)

taenidial fold

(Matusek et al., 2006)

Detailed Description

Statement

Reference

In the tracheal dorsal trunk of stage 17 Src42A^E1 heterozygoous and homozygous embryos, tanedial folds are similar to controls.

(Öztürk-Çolak et al., 2016)

Src42A^E1 mutants display occasional wandering/defasciculation in commissural axon pathfinding but remain ipsilateral in the embryonic central nervous system.

(O'Donnell and Bashaw, 2013)

Average Citrate Synthase activity is increased by 7% in heterozygous males and by 33% in Src42A^KG02515/Src42A^E1 transheterozygotes.

(Chen et al., 2008)

Mutant embryos show salivary gland guidance defects; they show ventral curving at the tip and they also show more generalised curving defects.

(Harris and Beckendorf, 2007)

Some Src42A^E1 larvae display a tracheal necrosis phenotype, while others display a superficially normal-looking tracheal tree, where cuticle secretion has been completed. These larvae also show an abnormal taenidial fold pattern.

(Matusek et al., 2006)

Src42A^E1 embryos show no salivary gland invagination defects.

(Chandrasekaran and Beckendorf, 2005)

75.7 +/- 3.6% of homozygotes die as embryos. Homozygous embryos have malformed mouth parts.

(Tateno et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Src42A^k10108/Src42A^E1 has abnormal neuroanatomy phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

NOT Enhanced by

Statement

Reference

Src42A^E1 has abnormal neuroanatomy phenotype, non-enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

NOT suppressed by

Statement

Reference

Src42A^E1 has abnormal neuroanatomy phenotype, non-suppressible by Src64B^ko

(O'Donnell and Bashaw, 2013)

Enhancer of

Statement

Reference

Src42A^E1, Src42A[+], Src64B[+], Src64B^PI is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Fak^K24/Fak^N30

(Tsai et al., 2008)

Src42A^E1 is an enhancer of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Src42A^E1 is an enhancer of partially lethal - majority die phenotype of DAAM^Ex1

(Matusek et al., 2006)

Suppressor of

Statement

Reference

Src42A^E1 is a suppressor of increased size | embryonic stage phenotype of emp^e3d1

(Pinheiro et al., 2023)

Other

Statement

Reference

Pez^CB-5380-3, Src42A^E1 has abnormal wound healing | embryonic stage phenotype

(Campbell et al., 2021)

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has abnormal neuroanatomy phenotype

(O'Donnell and Bashaw, 2013)

Src42A^E1/Src42A[+], Src64B^PI has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src42A^E1/Src42A[+] has abnormal neuroanatomy | third instar larval stage phenotype

(Tsai et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Src42A^k10108/Src42A^E1 has fascicle phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A^E1 has symmetrical commissure phenotype, enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

NOT Enhanced by

Statement

Reference

Src42A^E1 has fascicle phenotype, non-enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

Src42A^E1 has symmetrical commissure phenotype, non-enhanceable by Src64B^ko

(O'Donnell and Bashaw, 2013)

NOT suppressed by

Statement

Reference

Src42A^E1 has fascicle phenotype, non-suppressible by Src64B^ko

(O'Donnell and Bashaw, 2013)

Src42A^E1 has symmetrical commissure phenotype, non-suppressible by Src64B^ko

(O'Donnell and Bashaw, 2013)

Enhancer of

Statement

Reference

Src42A^E1, Src42A[+], Src64B[+], Src64B^PI is an enhancer of neuromuscular junction | third instar larval stage phenotype of Fak^K24/Fak^N30

(Tsai et al., 2008)

Src42A^E1 is an enhancer of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Src42A^E1 is an enhancer of embryonic/larval tracheal section phenotype of DAAM^Ex1

(Matusek et al., 2006)

Src42A^E1 is an enhancer of presumptive embryonic salivary gland phenotype of Btk^k00206

(Chandrasekaran and Beckendorf, 2005)

Suppressor of

Statement

Reference

Src42A^E1 is a suppressor of tracheal dorsal trunk primordium | embryonic stage phenotype of emp^e3d1

(Pinheiro et al., 2023)

Src42A^E1/Src42A^E1 is a suppressor of taenidial fold | embryonic stage 17 phenotype of Blimp-1^KG09531

(Öztürk-Çolak et al., 2016)

Src42A^E1 is a suppressor of embryonic/larval tracheal section phenotype of DAAM^C.UASp, Scer\GAL4^btl.PS

(Matusek et al., 2006)

Other

Statement

Reference

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has symmetrical commissure phenotype

(O'Donnell and Bashaw, 2013)

Src42A^k10108/Src42A^E1, Src64B[+]/Src64B^ko has fascicle phenotype

(O'Donnell and Bashaw, 2013)

Src42A^E1, p130CAS¹ has embryonic head phenotype

(Tikhmyanova et al., 2010)

Src42A^E1, p130CAS¹ has embryo | late embryonic stage phenotype

(Tikhmyanova et al., 2010)

Src42A^E1, p130CAS¹ has larva | first instar larval stage phenotype

(Tikhmyanova et al., 2010)

Src42A^E1/Src42A[+], p130CAS¹ has wing phenotype

(Tikhmyanova et al., 2010)

Src42A^E1/Src42A[+], Src64B^PI has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src42A^E1/Src42A[+] has neuromuscular junction | third instar larval stage phenotype

(Tsai et al., 2008)

Fak^N30/Fak^KG00304, Src42A^E1/Src42A[+] has NMJ bouton | increased number | third instar larval stage phenotype

(Tsai et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

In the tracheal dorsal trunk of stage 17 Src42A^E1 Src64B^ko double homozygous embryos, tanedial folds are similar to controls.

(Öztürk-Çolak et al., 2016)

Src42A^E1/Src42A^k10108 ; Src64B^ko/+ mutants display occasional wandering/defasciculation in commissural axon pathfinding but remain ipsilateral in the embryonic central nervous system.

Src42A^E1/Src42A^k10108 ; Src64B^ko/Src64B^ko double mutants exhibit severe defects in Fas2-positive axons, with Fas2-positive ipsilateral axons often crossing the midline inappropriately. eg-positive commissural neurons do not exhibit defects in these mutants.

Src42A^E1+; Src64B^ko mutants display occasional wandering/defasciculation in commissural axon pathfinding but remain ipsilateral in the embryonic central nervous system.

(O'Donnell and Bashaw, 2013)

The low percentage of Src42A^E1/+; p130CAS¹/p130CAS¹ adult escapers manifest wing blister defects.

Approximately 90% of Src42A^E1; p130CAS¹ double mutants have holes in or completely absent head cuticles and 10% of these embryos exhibit germ band retraction cuticle defects.

(Tikhmyanova et al., 2010)

Significant overgrowth of the neuromuscular junction (NMJ) (increased bouton number per muscle area and increased NMJ length per muscle area) is seen in Src42A^E1/+ Fak^N30/Fak^KG00304 third instar larvae.

Significant overgrowth of the NMJ (increased bouton number per muscle area) is seen in Src42A^E1/+ ; Src64B^PI/Src64B^PI third instar larvae.

The overgrowth at the NMJ (increased bouton number per muscle area) seen in Fak^N30/Fak^K24 third instar larvae is enhanced if they are also carrying both Src42A^E1/+ and Src64B^PI/+.

(Tsai et al., 2008)

The cuticle and semi-lethal phenotypes of DAAM^Ex1 larvae is enhanced by Src42A^E1.

The tracheal cuticle defects of larvae that express DAAM^{C.Scer\UAS.P\T} under the control of Scer\GAL4^btl.PS are dominantly suppressed by Src42A^E1.

(Matusek et al., 2006)

Btk29A^k00206; Src42A^E1 embryos show an enhanced salivary gland invagination phenotype compared to Btk29A^k00206 single mutants. More salivary glands show premature endoreplication in the double mutants. The double mutants also show the disorganised actin phenotype although it is not clear whether this is enhanced compared to Btk29A^k00206 embryos.

(Chandrasekaran and Beckendorf, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6408

w¹¹¹⁸; Src42A^E1/CyO

78533

w^*; Src42A^E1/CyO; TI{TI}Src64B^ko/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

(Kaltenbach et al., 2007)

Src42^E1

(Harris and Beckendorf, 2007)

Src42A^E1

(Tsarouhas et al., 2019, Öztürk-Çolak et al., 2016, O'Donnell and Bashaw, 2013, Tikhmyanova et al., 2010, Tsai et al., 2008, Matusek et al., 2006)

Src^E1

(Chen et al., 2008)

src42A^E1

(Pinheiro et al., 2023, Campbell et al., 2021, Tsarouhas et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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