FlyBase Allele Report: Dmel\sals[f07849]

General Information

Symbol

Dmel\sals^f07849

Species

D. melanogaster

Name

FlyBase ID

FBal0161776

Feature type

allele

Associated gene

Dmel\sals

Associated Insertion(s)

PBac{WH}sals^f07849

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bai et al., 2007)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}sals^f07849

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:11,439,150..11,439,150 [-]

(Farkas et al., 2024, Bai et al., 2007, Gene Disruption Project members and Exelixis, 2005, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | embryonic stage

(Bai et al., 2007)

lethal - all die during embryonic stage (with Df(3R)M-Kx1)

(Bai et al., 2007)

lethal - all die during embryonic stage | recessive

(Bai et al., 2007)

some die during embryonic stage (with Df(3R)M-Kx1)

(Bai et al., 2007)

some die during embryonic stage | recessive

(Bai et al., 2007)

Phenotype Manifest In

myofibril

(Bai et al., 2007)

sarcomere

(Bai et al., 2007)

striated muscle thin filament

(Bai et al., 2007)

Detailed Description

Statement

Reference

Homozygous and sals^f07849/Df(3R)M-Kx1 embryos fail to hatch from the egg case (100% penetrance) and show similar muscle defects.

Primary muscle cultures derived from myoblasts dissociated from homozygous mutant embryos have a reduced sarcomere length and myofibril width, but normal myofibril length compared to control cultures.

Stage 16 homozygous embryos have normal overall muscle morphology, and the number of nuclei in mutant muscles is comparable to that in wild type.

Muscles in late stage 17 mutant embryos have myofibrils with numerous short sarcomeres (they show an approximately 27% reduction in length compared to wild type). In addition, the mutant myofibrils are thin and split and are distributed along the lateral side of muscles. The thin filaments are significantly shorter in length than normal. The nuclei of the mutant muscles are localised more towards the muscle ends (wild-type muscles have even distributed nuclei).

The muscles of mutant larvae at 28-30 hours after egg laying contain many more sarcomeres than those of control larvae, but they are much shorter in length longitudinally compared to controls. Some of the mutant muscles show a complete disruption of myofibril morphology.

Peristaltic movement is detectable in later stage 17 mutant embryos, however, the mutant embryos have less frequent backward peristalsis than controls, and the vigour of their contraction is much reduced. At 28-30 hours after egg laying (the expected hatch time), the mutants show weak movement. They eventually die in the egg case within a day. If mutant animals are manually removed from the egg case, they are flaccid and immobile.

(Bai et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sals^f07849 is an enhancer of lethal | embryonic stage phenotype of Scer\GAL4^Mef2.PR, tmod^UAS.cBa

(Bai et al., 2007)

Phenotype Manifest In

Enhancer of

Statement

Reference

sals^f07849 is an enhancer of striated muscle thin filament phenotype of Scer\GAL4^Mef2.PR, tmod^UAS.cBa

(Bai et al., 2007)

NOT Enhancer of

Statement

Reference

sals^f07849/sals[+] is a non-enhancer of indirect flight muscle cell phenotype of DAAM^Ex1

(Molnár et al., 2014)

NOT Suppressor of

Statement

Reference

sals^f07849/sals[+] is a non-suppressor of indirect flight muscle cell phenotype of DAAM^Ex1

(Molnár et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The mild indirect muscle phenotype seen in DAAM^Ex1 mutants is not altered by sals^f07849/+.

(Molnár et al., 2014)

sals^f07849 enhances the lethality cause by expression of tmod^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.PR, such that a greater fraction of the animals die as embryos.

The reduction in thin filament length seen in primary cultured muscle cells isolated from embryos expressing tmod^Scer\UAS.cBa under the control of Scer\GAL4^Mef2.PR is increased if the animals are also carrying sals^f07849.

(Bai et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

sals^f07849 is rescued by sals^UAS.cBa/Scer\GAL4^Mef2.PR

(Bai et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19113

w¹¹¹⁸; PBac{WH}sals^f07849 CG17230^f07849/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Mobilisation of the insertion can fully revert the lethal phenotype.

(Bai et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG31374^f07849

sals^f07849

(Farkas et al., 2024, Molnár et al., 2014, Bai et al., 2007)

sals^null

(Farkas et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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