FlyBase Allele Report: Dmel\Mmp2[UAS.cPa]

Expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^Mef2.PU does not lead to a significant decrease in the migration velocity of embryonic cardioblasts, nor the number of lamellipodia at the cardioblast leading edge, but the number of filopodia at the leading edge shows a small but significant decrease, the regular cardioblast alignment is disrupted, and multiple ectopic lumens form at the heart proper region, as compared to wild type.

(Raza et al., 2017)

In females with expression of Mmp2^Scer\UAS.cPa driven by Scer\GAL4^GMR47A04, mature eggs rupture and most lack covering follicle cells.

(Deady et al., 2015)

Scer\GAL4^repo.PL + Scer\GAL4^repo.PU-mediated expression of Mmp2^Scer\UAS.cPa, activated at the onset of third instar larval stage via use of Scer\GAL80^ts.αTub84B, causes shape changes in the ventral nerve cord and deformation of the brain lobes 40h later. When induced from first instar onwards, viability is not affected and a more dramatic nervous system phenotype is produced.

Scer\GAL4^c527-mediated expression of Mmp2^Scer\UAS.cPa results an elongated ventral nerve cord. There is no loss of the ECM, but a rather wrinkled appearance.

(Meyer et al., 2014)

Expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^C587 (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to the adult stage) results in fused egg chambers which lack stalks (100% penetrance).

(Wang and Page-McCaw, 2014)

Pan-glial expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^repo.PU strongly increases ISNb and SNa fasciculation.

(Miller et al., 2011)

Flies expressing Mmp2^Scer\UAS.cPa in the female fat body under the control of Scer\GAL4^yolk are semi-lethal and infertile with a significantly shortened life span, dying a few days post eclosion at 18-20[o]C.

(Schmidt et al., 2011)

Scer\GAL4^elav.PU-mediated expression of Mmp2^Scer\UAS.cPa interferes with embryonic motor axon guidance: ISNb and SNa morphology/path-finding is disrupted, and exhibit phenotypes consistent with increased motor axon fasciculation. Expression via Scer\GAL4^repo.PU has similar effects but with higher penetrance; the number and position of repo-positive glia are unaffected. Mis-expression of Mmp2^Scer\UAS.cPa in mesoderm or in hemocytes does not result in motor axon phenotypes.

(Miller et al., 2008)

Mmp2^Scer\UAS.cPa; Scer\GAL4^GMR.PF flies have glassy eyes with flattened ommatidia and the occasional missing or double interommatidial bristles.

(Page-McCaw et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mmp2^UAS.cPa, Scer\GAL4^repo.PU has abnormal neuroanatomy | embryonic stage phenotype, suppressible by frac^Δ1

(Miller et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of decreased size | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

NOT Suppressor of

Statement

Reference

Scer\GAL4^repo.PU/Mmp2^UAS.cPa is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Mmp2^UAS.cPa, Scer\GAL4^C587 has stalk follicle cell phenotype, suppressible by Timp^UAS.cPa, Scer\GAL4^C587

(Wang and Page-McCaw, 2014)

Mmp2^UAS.cPa, Scer\GAL4^repo.PU has larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype, suppressible by frac^Δ1

(Miller et al., 2011)

Mmp2^UAS.cPa, Scer\GAL4^repo.PU has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, suppressible by frac^Δ1

(Miller et al., 2011)

Mmp2^UAS.cPa, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, suppressible | partially by Timp^UAS.cPa, Scer\GAL4^GMR.PF

(Page-McCaw et al., 2003)

Mmp2^UAS.cPa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible | partially by Timp^UAS.cPa, Scer\GAL4^GMR.PF

(Page-McCaw et al., 2003)

NOT Enhancer of

Statement

Reference

Scer\GAL4^C-765/Mmp2^UAS.cPa is a non-enhancer of wing disc | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Suppressor of

Statement

Reference

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of cuboidal/columnar epithelium | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of squamous epithelium | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of basement membrane | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of wing disc | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Scer\GAL4^coro-409/Mmp2^UAS.cPa is a suppressor | partially of wing pouch | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Mmp2^UAS.cPa/Scer\GAL4^GMR44E10 is a suppressor of egg chamber phenotype of Df(3R)BSC141/Oamb^MI12417

(Deady and Sun, 2015)

Scer\GAL80^ts.αTub84B, Mmp2^UAS.cPa, Scer\GAL4^btl.PS is a suppressor of adult tracheal air sac | third instar larval stage | heat sensitive phenotype of Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B, Timp^UAS.cPa

(Wang et al., 2010)

Scer\GAL80^ts.αTub84B, Mmp2^UAS.cPa, Scer\GAL4^btl.PS is a suppressor of dorsal air sac primordium tip cell | third instar larval stage | heat sensitive phenotype of Scer\GAL4^btl.PS, Scer\GAL80^ts.αTub84B, Timp^UAS.cPa

(Wang et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^C-765/Mmp2^UAS.cPa is a non-suppressor of wing disc | larval stage phenotype of trol^JO271.49

(Bonche et al., 2022)

Mmp2^UAS.cPa, Scer\GAL4^C587 is a non-suppressor of stalk follicle cell | increased number phenotype of Scer\GAL4^C587, wg^{UAS.cHa.Tag:HA}

(Wang and Page-McCaw, 2014)

Scer\GAL4^repo.PU/Mmp2^UAS.cPa is a non-suppressor of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Scer\GAL4^repo.PU/Mmp2^UAS.cPa is a non-suppressor of larval segmental nerve branch SNa of A1-7 | embryonic stage phenotype of frac^Δ1

(Miller et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The follicle rupture defects characteristic for Oamb^MI12417 mutant females are rescued by ectopic expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^GMR44E10.

(Deady and Sun, 2015)

Co-expression of Timp^Scer\UAS.cPa suppresses the fused egg chamber phenotype seen in females expressing Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^C587 (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to the adult stage) and restores stalk cell numbers to normal.

Co-expression of Mmp2^Scer\UAS.cPa has no effect on the increase in stalk cell numbers seen in females expressing wg^{Scer\UAS.cHa.T:Ivir\HA1} under the control of Scer\GAL4^C587 (using the temperature sensitive Scer\GAL80^ts.αTub84B allele to limit expression to the adult stage).

(Wang and Page-McCaw, 2014)

A homozygous frac^Δ1 mutant background suppresses the excess fasciculation seen in embryos over-expressing Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^repo.PU. Furthermore, these embryos display diminished motor axon bundling similar to that observed in frac^Δ1 homozygotes.

(Miller et al., 2011)

Expression of Mmp2^Scer\UAS.cPa in third instar larvae expressing Timp^Scer\UAS.cPa under the control of Scer\GAL4^btl.PS (by inactivating Scer\GAL80^{ts.alphaTub84B} through heat-shock), largely reverts the air sac primordium defects observed when Timp^Scer\UAS.cPa is expressed alone.

(Wang et al., 2010)

The eye phenotypes due to Mmp2^Scer\UAS.cPa; Scer\GAL4^GMR.PF are almost completely suppressed by the presence of one copy of Timp^Scer\UAS.cPa.

(Page-McCaw et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Mef2.PU/Mmp2^UAS.cPa partially rescues Mmp2^W307stop

(Raza et al., 2017)

Comments

Expression of Mmp2^Scer\UAS.cPa under the control of Scer\GAL4^Mef2.PU largely rescues the heart development defects (including cardioblast migration velocity, filopodial and lamellipodial activity, and lumen formation) seen in Mmp2^W307stop/Mmp2^W307stop mutant embryos.

(Raza et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (3)

58704

58705

58706