FlyBase Allele Report: Scer\GAL4[repo.PL]

General Information

Symbol

Scer\GAL4^repo.PL

Species

S. cerevisiae

Name

repo promoter construct of Lee

FlyBase ID

FBal0191674

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{repo-Gal4.L}

Also Known As

repo-GAL4, repoGal4, repo4.3-Gal4, repo4.3Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Lee and Jones, 2005)

Progenitor genotype

Carried in construct

P{repo-Gal4.L}

Cytology

Description

Expression of a GAL4 driver is under the control of 4.2Kb of genomic sequence from immediately upstream of repo.

(Lee and Jones, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

repo

repo_4.3

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

embryonic/larval glial cell

(Lee and Jones, 2005)

larval stage

embryonic/larval glial cell

(Schmidt et al., 2012)

third instar larval stage

embryonic/larval glial cell

(Kerr et al., 2014)

pupal stage

glial cell

(Hilu-Dadia et al., 2018)

adult stage

adult glial cell

(Tanenhaus et al., 2012)

Additional Information

Statement

Reference

Drives expression in glial cells at all stages.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

repo

(Kerr et al., 2014, Schmidt et al., 2012, Tanenhaus et al., 2012, Lee and Jones, 2005)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal locomotor behavior | adult stage, with βTub60D^GD10956, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal locomotor behavior | larval stage, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal locomotor behavior | larval stage, with βTub60D^GD10956, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal neuroanatomy, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal neuroanatomy | adult stage, with Nrg^167.UAS

(Clements et al., 2021)

abnormal neuroanatomy | adult stage, with Nrg^GD82

(Clements et al., 2021)

abnormal neuroanatomy | adult stage, with PlexA^HM05221

(Clements et al., 2021)

abnormal neuroanatomy | adult stage, with Sema1a^HMS01307

(Clements et al., 2021)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo.PU, if^GD1175

(Meyer et al., 2014)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo.PU, mys^RNAi.UAS.cMa

(Meyer et al., 2014)

abnormal neuroanatomy | third instar larval stage | heat sensitive, with Mmp2^UAS.cPa, Scer\GAL4^repo.PU, Scer\GAL80^ts.αTub84B

(Meyer et al., 2014)

abnormal neurophysiology | third instar larval stage, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

abnormal starvation stress response | adult stage, with Nrg^GD82

(Clements et al., 2021)

abnormal starvation stress response | adult stage, with Sema1a^HMS01307

(Clements et al., 2021)

decreased rate of movement | adult stage, with Jra^KK100582, Scer\GAL4^repo

(Schmidt et al., 2012)

flightless, with Alp12^NIG.16771R, Scer\GAL4^repo

(Schmidt et al., 2012)

increased cell number | third instar larval stage, with fray^KK100424

(Matzat et al., 2015)

lethal, with Khc^GD12278, Scer\GAL4^elav-C155, Scer\GAL4^repo

(Schmidt et al., 2012)

lethal, with ogre^KK104770

(Volkenhoff et al., 2018)

lethal - all die before end of larval stage, with Inx2^JF02446

(Volkenhoff et al., 2018)

lethal - all die before end of larval stage, with Inx2^KK111067

(Volkenhoff et al., 2018)

lethal - all die before end of P-stage, with ogre^HMS02764

(Volkenhoff et al., 2018)

lethal - all die during embryonic stage, with Mmp1^f2.UAS, Scer\GAL4^repo.PU

(Meyer et al., 2014)

lethal - all die during embryonic stage, with Mmp2^UAS.cPa, Scer\GAL4^repo.PU

(Meyer et al., 2014)

lethal - all die during larval stage, with svp^GD1546

(Sieglitz et al., 2013)

lethal - all die during larval stage | heat sensitive, with Mmp1^f2.UAS, Scer\GAL4^repo.PU, Scer\GAL80^ts.αTub84B

(Meyer et al., 2014)

long lived, with Inx2^JF02446

(Volkenhoff et al., 2018)

long lived, with ogre^HMS02764

(Volkenhoff et al., 2018)

partially lethal - majority die, with Khc⁸, Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

partially lethal - majority die, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

short lived | nutrition conditional, with Inx2^JF02446

(Volkenhoff et al., 2018)

short lived | nutrition conditional, with ogre^HMS02764

(Volkenhoff et al., 2018)

Phenotype Manifest In

adult insulin secreting cell of pars intercerebralis, with Nrg^167.UAS

(Clements et al., 2021)

adult insulin secreting cell of pars intercerebralis, with Nrg^GD82

(Clements et al., 2021)

adult insulin secreting cell of pars intercerebralis, with PlexA^HM05221

(Clements et al., 2021)

adult insulin secreting cell of pars intercerebralis, with Sema1a^HMS01307

(Clements et al., 2021)

embryonic/larval brain | third instar larval stage | heat sensitive, with Mmp2^UAS.cPa, Scer\GAL4^repo.PU, Scer\GAL80^ts.αTub84B

(Meyer et al., 2014)

embryonic/larval CNS subperineurial glial cell | third instar larval stage, with vn^GD2321

(Matzat et al., 2015)

embryonic/larval glial cell, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

embryonic/larval glial cell, with Miro^KK102189, Scer\GAL4^repo

(Schmidt et al., 2012)

embryonic/larval glial cell, with Scer\GAL4^repo, milt^GD8116

(Schmidt et al., 2012)

embryonic/larval optic stalk surface glial cell | third instar larval stage, with sty^UAS.cHa

(Sieglitz et al., 2013)

embryonic/larval optic stalk surface glial cell | third instar larval stage, with svp^GD1546

(Sieglitz et al., 2013)

embryonic/larval wrapping glial cell | third instar larval stage, with Egfr^DN.UAS.cBa

(Matzat et al., 2015)

embryonic/larval wrapping glial cell | third instar larval stage, with fray^KK100424

(Matzat et al., 2015)

embryonic/larval wrapping glial cell | third instar larval stage, with spi^s.UAS

(Matzat et al., 2015)

embryonic/larval wrapping glial cell | third instar larval stage, with vn^GD2321

(Matzat et al., 2015)

fascicle | third instar larval stage, with sty^GD1156

(Sieglitz et al., 2013)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^repo.PU, if^GD1175

(Meyer et al., 2014)

larval ventral nerve cord | third instar larval stage, with Scer\GAL4^repo.PU, mys^RNAi.UAS.cMa

(Meyer et al., 2014)

larval ventral nerve cord | third instar larval stage | heat sensitive, with Mmp2^UAS.cPa, Scer\GAL4^repo.PU, Scer\GAL80^ts.αTub84B

(Meyer et al., 2014)

mitochondrion, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

mitochondrion, with Miro^KK102189, Scer\GAL4^repo

(Schmidt et al., 2012)

mitochondrion, with Scer\GAL4^repo, milt^GD8116

(Schmidt et al., 2012)

nerve | third instar larval stage, with fray^KK100424

(Matzat et al., 2015)

neurite | adult stage, with Nrg^167.UAS

(Clements et al., 2021)

neurite | adult stage | ectopic, with Nrg^GD82

(Clements et al., 2021)

neurite | adult stage | ectopic, with PlexA^HM05221

(Clements et al., 2021)

neurite | adult stage | ectopic, with Sema1a^HMS01307

(Clements et al., 2021)

peripheral nervous system, with Khc^GD12278, Scer\GAL4^repo

(Schmidt et al., 2012)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^repo.PL, svp^GD1546 has lethal - all die during larval stage phenotype, suppressible by sty^GD1156/Scer\GAL4^repo.PL

(Sieglitz et al., 2013)

Khc^GD12278, Scer\GAL4^repo.PL, Scer\GAL4^repo has abnormal locomotor behavior | adult stage phenotype, suppressible by Dana\Khc^UAS.RR, Scer\GAL4^repo.PL, Scer\GAL4^repo

(Schmidt et al., 2012)

Khc^GD12278, Scer\GAL4^repo.PL, Scer\GAL4^repo has abnormal locomotor behavior | larval stage phenotype, suppressible by Dana\Khc^UAS.RR, Scer\GAL4^repo.PL, Scer\GAL4^repo

(Schmidt et al., 2012)

Suppressor of

Statement

Reference

Mmus\Slc5a2^UAS.cYa/Scer\GAL4^repo.PL is a suppressor of female sterile phenotype of bumpel², kumpel²

(Yildirim et al., 2022)

sty^GD1156/Scer\GAL4^repo.PL is a suppressor of lethal - all die during larval stage phenotype of Scer\GAL4^repo.PL, svp^GD1546

(Sieglitz et al., 2013)

Dana\Khc^UAS.RR, Scer\GAL4^repo.PL, Scer\GAL4^repo is a suppressor of abnormal locomotor behavior | adult stage phenotype of Khc^GD12278, Scer\GAL4^repo.PL, Scer\GAL4^repo

(Schmidt et al., 2012)

Dana\Khc^UAS.RR, Scer\GAL4^repo.PL, Scer\GAL4^repo is a suppressor of abnormal locomotor behavior | larval stage phenotype of Khc^GD12278, Scer\GAL4^repo.PL, Scer\GAL4^repo

(Schmidt et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^repo.PL, sty^UAS.cHa has embryonic/larval optic stalk surface glial cell | third instar larval stage phenotype, enhanceable by rau^GD14083/Scer\GAL4^repo.PL

(Sieglitz et al., 2013)

Enhancer of

Statement

Reference

rau^GD14083/Scer\GAL4^repo.PL is an enhancer of embryonic/larval optic stalk surface glial cell | third instar larval stage phenotype of Scer\GAL4^repo.PL, sty^UAS.cHa

(Sieglitz et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Nrg^167.UAS/Scer\GAL4^repo.PL rescues Nrg⁸⁴⁹

(Clements et al., 2021)

Scer\GAL4^repo.PL/lace^{WT.UAS.Tag:HA} rescues lace²/lace^k05305

(Goyal et al., 2019)

Partially rescues

repo^UAS.cLa/Scer\GAL4^repo.PL partially rescues repo⁶⁴

(Lee and Jones, 2005)

Fails to rescue

Scer\GAL4^repo.PL/ND-23^UAS.cLa fails to rescue ND-23⁶⁰¹¹⁴/Df(3R)Exel8162

(Loewen and Ganetzky, 2018)

Scer\GAL4^repo, Scer\GAL4^repo.PL, Khc^UAS.cSa fails to rescue Khc⁸/Df(2R)Jp6

(Schmidt et al., 2012)

Scer\GAL4^repo.PL/GlcAT-P^UAS.cPa fails to rescue GlcAT-P^brv1

(Pandey et al., 2011)

Scer\GAL4^repo.PL/β-Spec^UAS.cHa fails to rescue β-Spec^unspecified

(Hülsmeier et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

602908

w¹¹¹⁸; P{repo-Gal4.L}Oatp30B^repoF3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^repo.PL

Name Synonyms

repo promoter construct of Lee

(Lee and Jones, 2005)

Secondary FlyBase IDs

References (34)

Report Sections

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