FlyBase Allele Report: Dmel\E2f1[su89]

General Information

Symbol

Dmel\E2f1^su89

Species

D. melanogaster

Name

FlyBase ID

FBal0141831

Feature type

allele

Associated gene

Dmel\E2f1

Associated Insertion(s)

Carried in Construct

Also Known As

de2f1^su89

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Single point mutation in the Rbf protein binding domain.

(Du et al., 2003)

Amino acid replacement: L786Q.

(Weng et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,621,630..21,621,630 [-]

Nucleotide change:

T21621630A

Amino acid change:

Reported amino acid change:

L786Q

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Weng et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Du et al., 2003, Weng et al., 2003)

viable

(Du et al., 2003, Weng et al., 2003)

visible

(Du et al., 2003)

Phenotype Manifest In

eye

(Du et al., 2003, Weng et al., 2003)

interommatidial bristle | ectopic

(Weng et al., 2003)

wing disc | wandering third instar larval stage

(Moon et al., 2005)

Detailed Description

Statement

Reference

In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2f^su89 homozygotes, some apoptotic cells are present in both prospective vein and DV boundary regions.

(Moon et al., 2005)

Mutant flies have minor defects in the eye.

(Du et al., 2003)

Homozygotes are viable, fertile with normal macrochaetae on the notum. Mutants do have a very slight eye phenotype with extra bristles around some of the ommatidia.

(Weng et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

E2f1^su89 is a non-enhancer of increased cell number | pupal stage | somatic clone phenotype of rno³

(Steele et al., 2009)

Suppressor of

Statement

Reference

E2f1^su89 is a suppressor of decreased occurrence of cell division | larval stage phenotype of Rbf^280.GMR, dap^GMR.PdN

(Weng et al., 2003)

E2f1^su89 is a suppressor of decreased occurrence of cell division | larval stage phenotype of Rbf^GMR.PD, dap^GMR.PdN

(Weng et al., 2003)

Other

Statement

Reference

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has lethal phenotype

(Weng et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has macrochaeta & mesothoracic tergum phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & G1 phase phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & nucleus phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

Enhancer of

Statement

Reference

E2f1^su89 is an enhancer of salivary gland & nucleus phenotype of E2f2^p111-5/E2f2^76Q1

(Weng et al., 2003)

NOT Enhancer of

Statement

Reference

E2f1^su89 is a non-enhancer of photoreceptor cell R8 | third instar larval stage | somatic clone phenotype of rno¹

(Steele et al., 2009)

Suppressor of

Statement

Reference

E2f1^su89 is a suppressor of eye disc & S phase phenotype of Rbf^280.GMR, dap^GMR.PdN

(Weng et al., 2003)

E2f1^su89 is a suppressor of anterior crossvein phenotype of Rbf^UAS.cDa, Scer\GAL4^dpp.blk1

(Weng et al., 2003)

E2f1^su89 is a suppressor of submarginal cell phenotype of Rbf^UAS.cDa, Scer\GAL4^dpp.blk1

(Weng et al., 2003)

E2f1^su89 is a suppressor of interommatidial bristle phenotype of Rbf^GMR.PD

(Weng et al., 2003)

E2f1^su89 is a suppressor of ommatidium phenotype of Rbf^GMR.PD

(Weng et al., 2003)

E2f1^su89 is a suppressor of eye disc & S phase phenotype of Rbf^GMR.PD, dap^GMR.PdN

(Weng et al., 2003)

Other

Statement

Reference

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has trichogen cell phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has cell cycle & scutellum phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has macrochaeta & mesothoracic tergum phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & G1 phase phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & nucleus phenotype

(Weng et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The shiny-eye phenotype and multiple photoreceptor-R8 phenotype observed in rno¹ somatic clones in the eye is not enhanced in a E2f^su89 background.

(Steele et al., 2009)

Rbf^280.GMR when combined with dap^GMR.PdN can inhibit or delay S phase entry during the second mitotic wave in developing eye disc. The introduction of E2f^su89 restores normal S phase entry in the second wave. dap^GMR.PdN enhances the eye phenotype seen in dap^GMR.PdN animals. These phenotypes are also suppressed by the addition of E2f^su89. The addition of E2f2^76Q1/E2f2^p111-5 to homozygous E2f^su89 leads to lethality in the majority of mutants - 75% of the mutants die during the larvae stage, 24% during the pupal stage, with only 1% surviving to adulthood. Larval development is asynchronous: while most larvae are smaller, some healthy larvae can reach sizes similar to wild-type, and their development is only delayed about a day. The surviving adults do not show gross defects in their adult structures, though 88% display extremely short macrochaetae on the notum. These macrochaetae form the proper structure but fail to grow to its normal size. This seems to be a result of a defect in endoreplication in those cells. Mutants also exhibit defects in maintaining cell cycle arrest in the scutella. Third instar larval salivary glands are also smaller in these animals. The number of cells is wild-type, but the DNA content of those cells is about 40% of wild-type, probably due to fewer rounds of endocycles. The length of each endocycle is correspondingly longer due to an increased length of the gap phase. The addition of CycE^AR95 to E2f2^76Q1/E2f2^p111-5, E2f^su89, partially suppresses the salivary gland endoreplication defect. Salivary gland nuclei in these animals have about 70% of the DNA content of wild-type. The lethality is also partially suppressed - 52% of animals survive to the pupal stage (compared to 25%). Finally the macrochaetae phenotype is strongly suppressed. - 99% of flies show normal macrochaetae (as opposed to 12%).

(Weng et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E2f1^su89

E2f^su89

dE2F1^su89

(Steele et al., 2009, Du et al., 2003)

de2f1^su89

(Moon et al., 2005)

e2f1^su89

(Zhang et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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