FlyBase Allele Report: Dmel\Rbf[280.GMR]

General Information

Symbol

Dmel\Rbf^280.GMR

Species

D. melanogaster

Name

FlyBase ID

FBal0144636

Feature type

allele

Associated gene

Dmel\Rbf

Associated Insertion(s)

Carried in Construct

P{GMR-Rbf.280}

Also Known As

GMRRBF-280²

Key Links

Transgenic product class

missense_variant

(Xin et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Xin et al., 2002)

Mutagen

in vitro construct

(Xin et al., 2002)

Progenitor genotype

Carried in construct

P{GMR-Rbf.280}

Cytology

Description

GMR regulatory sequences drive expression of a mutated form of Rbf; carries the amino acid replacements T356A, S728A, S760A and S771A.

(Xin et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Rbf

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage

(Xin et al., 2002)

visible

(Xin et al., 2002)

Phenotype Manifest In

eye

(Weng et al., 2003, Xin et al., 2002)

eye disc

(Xin et al., 2002)

interommatidial bristle

(Xin et al., 2002)

ommatidium

(Xin et al., 2002)

Detailed Description

Statement

Reference

Expression of Rbf^280.GMR results in the development of a very rough eye. Many ommatidia are fused and there is an almost complete loss of bristles. The ommatidia are variable in shape. The second mitotic wave is not blocked in flies carrying Rbf^280.GMR, but it is broader than wild type and BrdU staining is weaker. There are average of 125 +/- 13 S phase cells in the broader second mitotic wave, compared to 73 +/- 9 cells S phase cells in the wild-type second mitotic wave. Mitotic cells in the eye disc lose synchrony in animals carrying Rbf^280.GMR, and there are mitotic cells scattered throughout the posterior of the eye disc, in contrast to wild type. There are an average of 32 +/- 5 M phase cells in the mutant eye disc, compared to 37 +/-3 M phase cells in wild type.

(Xin et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^280.GMR has decreased occurrence of cell division | larval stage phenotype, enhanceable by dap^GMR.PdN

(Xin et al., 2002)

Suppressed by

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

Other

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has decreased occurrence of cell division | larval stage phenotype

(Weng et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbf^280.GMR has phenotype, enhanceable by dap^GMR.PdN

(Xin et al., 2002)

Suppressed by

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has eye disc & S phase phenotype, suppressible by E2f1^su89

(Weng et al., 2003)

NOT suppressed by

Statement

Reference

Rbf^280.GMR has phenotype, non-suppressible by dap^unspecified/dap[+]

(Xin et al., 2002)

Other

Statement

Reference

Rbf^280.GMR, dap^GMR.PdN has eye disc & S phase phenotype

(Weng et al., 2003)

Rbf^280.GMR, dap^GMR.PdN has eye disc phenotype

(Xin et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dap^GMR.PdN and Rbf^280.GMR completely inhibits BrdU incorporation in the second mitotic wave in the eye disc.

(Xin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rbf^280.GMR

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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