FlyBase Allele Report: Dmel\E2f2[76Q1]

General Information

Symbol

Dmel\E2f2^76Q1

Species

D. melanogaster

Name

FlyBase ID

FBal0126597

Feature type

allele

Associated gene

Dmel\E2f2

Associated Insertion(s)

Carried in Construct

Also Known As

de2f2^76Q1, E2f2^76Q.1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Frolov et al., 2001)

Progenitor genotype

Df(2L)Q3.1

(Frolov et al., 2001)

Cytology

Description

A deletion extends from 378 bp upstream of the E2f2 transcription start site to amino acid 5 of the coding sequence.

(Frolov et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:21,156,029..21,156,623 [-]

Comment:

Approximate endpoints of excision deletion reported as extending from a position 378bp upstream of the E2f2 transcription start to amino acid 5 of E2f2.

(Frolov et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal

(Frolov et al., 2001)

partially lethal - majority live (with Df(2L)pr-A14)

(Frolov et al., 2001)

partially lethal - majority live (with Df(2L)TW1)

(Frolov et al., 2001)

semi-fertile

(Frolov et al., 2001)

Phenotype Manifest In

egg chorion

(Frolov et al., 2001)

salivary gland & nucleus (with E2f2^p111-5)

(Weng et al., 2003)

Detailed Description

Statement

Reference

E2f2^76Q1/E2f2³²⁹ Mpp6^+t4 animals have a pattern of irradiation induced apoptosis in the late third instar wing disc which is identical to that seen in wild-type animals. (Mpp6^+t4 is present to rescued loss of Mpp6 in Df(2L)E2f2³²⁹.

(Moon et al., 2005)

20% to 35% of homozygotes survive to adulthood. These flies have reduced fertility. Only 50% of eggs laid by homozygous mothers hatch, and about 5% of eggs laid by homozygous females have fragile, thin chorions.

(Frolov et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

E2f2[+]/E2f2^76Q1 is a suppressor of visible | dominant phenotype of gcm^Pyx

(Popkova et al., 2012)

Df(2L)G5.1/E2f2^76Q1 is a suppressor of abnormal developmental rate phenotype of E2f1⁹¹/E2f1^rM729

(Frolov et al., 2001)

Other

Statement

Reference

E2f1⁹¹/E2f1^rM729, E2f2^g5/E2f2^76Q1 has decreased cell death | wandering third instar larval stage phenotype

(Moon et al., 2005)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has lethal phenotype

(Weng et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

E2f2^p111-5/E2f2^76Q1 has salivary gland & nucleus phenotype, enhanceable by E2f1^su89

(Weng et al., 2003)

Suppressed by

Statement

Reference

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has macrochaeta & mesothoracic tergum phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & G1 phase phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & nucleus phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland phenotype, suppressible by CycE^AR95

(Weng et al., 2003)

Suppressor of

Statement

Reference

E2f2[+]/E2f2^76Q1 is a suppressor of chaeta | increased number phenotype of gcm^Pyx

(Popkova et al., 2012)

Df(2L)G5.1/E2f2^76Q1 is a suppressor of imaginal disc phenotype of E2f1⁹¹/E2f1^rM729

(Frolov et al., 2001)

Other

Statement

Reference

E2f1⁹¹/E2f1^rM729, E2f2^c03344/E2f2^76Q1 has larval fat cell of larval fat body | third instar larval stage phenotype

(Zappia et al., 2021)

E2f1⁹¹/E2f1^rM729, E2f2^c03344/E2f2^76Q1 has nucleus | increased number | third instar larval stage phenotype

(Zappia et al., 2021)

E2f1⁹¹/E2f1^rM729, E2f2^g5/E2f2^76Q1 has wing disc | wandering third instar larval stage phenotype

(Moon et al., 2005)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has trichogen cell phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has cell cycle & scutellum phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has macrochaeta & mesothoracic tergum phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & G1 phase phenotype

(Weng et al., 2003)

E2f1^su89, E2f2^p111-5/E2f2^76Q1 has salivary gland & nucleus phenotype

(Weng et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

E2f2^76Q1/E2f2^c03344, E2f1⁹¹/E2f1^rM729 third instar larvae exhibit binucleated cells in the fat body.

(Zappia et al., 2021)

The addition of E2f2^76Q1/E2f2^p111-5 to homozygous E2f^su89 leads to lethality in the majority of mutants - 75% of the mutants die during the larvae stage, 24% during the pupal stage, with only 1% surviving to adulthood. Larval development is asynchronous: while most larvae are smaller, some healthy larvae can reach sizes similar to wild-type, and their development is only delayed about a day. The surviving adults do not show gross defects in their adult structures, though 88% display extremely short macrochaetae on the notum. These macrochaetae form the proper structure but fail to grow to its normal size. This seems to be a result of a defect in endoreplication in those cells. Mutants also exhibit defects in maintaining cell cycle arrest in the scutella. Third instar larval salivary glands are also smaller in these animals. The number of cells is wild-type, but the DNA content of those cells is about 40% of wild-type, probably due to fewer rounds of endocycles. The length of each endocycle is correspondingly longer due to an increased length of the gap phase. The addition of CycE^AR95 to E2f2^76Q1/E2f2^p111-5, E2f^su89, partially suppresses the salivary gland endoreplication defect. Salivary gland nuclei in these animals have about 70% of the DNA content of wild-type. The lethality is also partially suppressed - 52% of animals survive to the pupal stage (compared to 25%). Finally the macrochaetae phenotype is strongly suppressed. - 99% of flies show normal macrochaetae (as opposed to 12%).

(Weng et al., 2003)

The addition of E2f2^76Q1/Df(2L)G5.1 to E2f⁹¹/E2f^rM729 flies almost completely suppresses the E2f⁹¹/E2f^rM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The pattern of DNA synthesis in eye discs is largely normal.

(Frolov et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7436

w^*; E2f2^76Q1 cn¹ bw¹/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

This allele was made by a duplication event caused by the excision of P{lacW}Mpp6^k16403 that replaces the 5' UTR sequences removed in Df(2L)Q3.1.

(Frolov et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

E2f2^76Q.1

(Weng et al., 2003)

E2f2^76Q1

(Zappia et al., 2021, Santana et al., 2020, Zappia and Frolov, 2016, Baumgardt et al., 2014)

de2f2^76Q1

(Moon et al., 2005, Dimova et al., 2003, Frolov et al., 2003)

e2f2^76Q.1

(Miles et al., 2014)

e2f2^76Q1

(Zhang et al., 2021)

e2f2^76Q

(Stevaux et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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