FlyBase Allele Report: Dmel\dap[UAS.cdNa]

General Information

Symbol

Dmel\dap^UAS.cdNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of de Nooij

FlyBase ID

FBal0141198

Feature type

allele

Associated gene

Dmel\dap

Associated Insertion(s)

Carried in Construct

P{UAS-dap.dN}

Also Known As

UAS-dap, UAS-dacapo, UAS:Dacapo

Key Links

Transgenic product class

wild_type

(Tseng and Hariharan, 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tseng and Hariharan, 2002)

Mutagen

in vitro construct

(Tseng and Hariharan, 2002)

Progenitor genotype

Carried in construct

P{UAS-dap.dN}

Cytology

Description

UAS regulatory sequences drive expression of dap.

(Tseng and Hariharan, 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

exacerbates tauopathy

modeled by Hsap\MAPT^{S202A.T205A.UAS}, Rbf^UAS.cDa

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{S396A.S404A.UAS}, Rbf^UAS.cDa

(Steinhilb et al., 2007)

modeled by Hsap\MAPT^{T231A.S235A.UAS}, Rbf^UAS.cDa

(Steinhilb et al., 2007)

modeled by CycA^UAS.cWa, Hsap\MAPT^R406W.UAS

(Khurana et al., 2006)

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa, Rbf^UAS.cDa

(Steinhilb et al., 2007)

modeled by Cdk1^E51Q.UAS, Hsap\MAPT^R406W.UAS

(Khurana et al., 2006)

modeled by Rbf^UAS.cDa, Hsap\MAPT^R406W.UAS

(Khurana et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^en.PU

(Zielke et al., 2014)

abnormal mitotic cell cycle | embryonic stage, with Scer\GAL4^en-e16E

(Parker, 2006)

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

abnormal size | third instar larval stage, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

decreased cell death | embryonic stage, with Scer\GAL4^en-e16E

(Parker, 2006)

decreased occurrence of cell division | larval stage, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

lethal, with Scer\GAL4^ap-md544

(Morris et al., 2006)

lethal, with Scer\GAL4^Dll-md23

(Morris et al., 2006)

lethal, with Scer\GAL4^T80

(Morris et al., 2006)

lethal, with Scer\GAL4^αTub84B.PL

(Morris et al., 2006)

partially lethal - majority die, with Scer\GAL4^32B

(Morris et al., 2006)

visible, with Scer\GAL4^en-e16E

(Morris et al., 2006, Tseng and Hariharan, 2002)

visible, with Scer\GAL4^ey.PH

(Morris et al., 2006, Tseng and Hariharan, 2002)

visible, with Scer\GAL4^GMR.PF

(Morris et al., 2006)

Phenotype Manifest In

embryonic segment posterior compartment, with Scer\GAL4^en-e16E

(Parker, 2006)

eye, with Scer\GAL4^ey.PH

(Morris et al., 2006, Tseng and Hariharan, 2002)

eye, with Scer\GAL4^GMR.PF

(Morris et al., 2006)

eye disc, with Scer\GAL4^ey.PH

(Tseng and Hariharan, 2002)

follicle cell & nucleus | somatic clone, with Scer\GAL4^Act5C.PP

(Shcherbata et al., 2004)

follicle stem cell | somatic clone

(Wang and Kalderon, 2009)

mitotic cell cycle, with Scer\GAL4^Act5C.PP

(Reis and Edgar, 2004)

mitotic cell cycle, with Scer\GAL4^unspecified

(Reis and Edgar, 2004)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Tseng and Hariharan, 2002)

wing cell, with Scer\GAL4^en-e16E

(Morris et al., 2006)

Detailed Description

Statement

Reference

Scer\GAL4^en.PU-mediated expression increases the fraction of wing disc cells in G1 phase and concomitantly decreases the fraction in G2.

(Zielke et al., 2014)

Very few ovarioles accumulate more than one follicle stem cell (FSC) expressing Scer\GAL4^tub-driven dap^{Scer\UAS.cdNa}, indicating a modest FSC defect.

(Wang and Kalderon, 2009)

Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^en-e16E in the posterior compartment cells of embryos causes these cells to arrest in G1 and prevents their death through apoptosis.

(Parker, 2006)

Cells expressing dap^{Scer\UAS.cdNa} have longer G1 phases than wild-type, though cell doubling times are not significantly different from wild-type, suggesting their G2 and S phases are shorter.

(Reis and Edgar, 2004)

Ovarian follicle cells in somatic clones of dap^{Scer\UAS.cdNa}; Scer\GAL4^Act5C.PP after stage 6 of oogenesis have small nuclei and do not incorporate BrdU indicating a lack of S phases.

(Shcherbata et al., 2004)

Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^ey.PH results in smaller third larval instar eye discs than normal and eyes which are severely reduced in size. Flies expressing dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^hs.2sev have wild-type eyes. Expression of dap^{Scer\UAS.cdNa} under the control of Scer\GAL4^en-e16E results in reduced cell division in the posterior compartment of the wing.

(Tseng and Hariharan, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of abnormal size phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of increased cell death | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

dap^UAS.cdNa, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of increased cell death | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Cdk1^E51Q.UAS, Scer\GAL4^elav.PLu is a suppressor of increased cell death | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa, Scer\GAL4^αTub84B.PL is a suppressor of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^αTub84B.PL, scrib¹

(Brumby and Richardson, 2003)

dap^UAS.cdNa is a suppressor of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^αTub84B.PL, scrib¹

(Brumby and Richardson, 2003)

NOT Suppressor of

Statement

Reference

dap^UAS.cdNa, Scer\GAL4^Act88F.PD is a non-suppressor of visible phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2006)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, dap^UAS.cdNa has abnormal DNA repair | larval stage | conditional phenotype

(Dekanty et al., 2015)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has abnormal mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

dap^UAS.cdNa/Scer\GAL4^Tub.PU is an enhancer of follicle stem cell | somatic clone phenotype of CycE^WX

(Wang and Kalderon, 2009)

Suppressor of

Statement

Reference

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of eye disc | posterior | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of photoreceptor cell R7 | third instar larval stage phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa/Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, p53^GUS.PB

(Fan et al., 2010)

dap^UAS.cdNa, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

dap^UAS.cdNa, Scer\GAL4^repo.PU is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S202A.T205A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{T231A.S235A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^{S396A.S404A.UAS}, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Steinhilb et al., 2007)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa/Cdk1^E51Q.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Scer\GAL4^GMR.PF, dap^UAS.cdNa/Rbf^UAS.cDa is a suppressor of eye phenotype of Hsap\MAPT^V337M.UAS, Rheb^AV4, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa, Scer\GAL4^GMR.PF, Rbf^UAS.cDa is a suppressor of eye | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^E14.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of adult brain | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^elav.PLu is a suppressor of lamina | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

dap^UAS.cdNa/Cdk1^E51Q.UAS, Scer\GAL4^elav.PLu is a suppressor of adult brain | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

NOT Suppressor of

Statement

Reference

dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

dap^UAS.cdNa, Scer\GAL4^Act88F.PD is a non-suppressor of wing blade phenotype of E2f1^UAS.cNa, Scer\GAL4^Act88F.PD

(Morris et al., 2006)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, dap^UAS.cdNa has wing disc posterior compartment | conditional phenotype

(Dekanty et al., 2015)

Dp^a1/Dp^a2, Scer\GAL4^Act5C.PP, dap^UAS.cdNa has mitotic cell cycle phenotype

(Reis and Edgar, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dap^{Scer\UAS.cdNa} completely suppresses the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU. The gross neural morphogenesis defects are also suppressed.

(Read et al., 2009)

Expression of Scer\GAL4^tub-driven dap^{Scer\UAS.cdNa} completely suppresses the formation of large transient CycE^WX follicle cell clones.

(Wang and Kalderon, 2009)

Cells expressing dap^{Scer\UAS.cdNa} driven by Scer\GAL4^Act5C.PP in a Dp^a1/Dp^a2 background, divide more slowly than controls.

(Reis and Edgar, 2004)

The addition of dap^{Scer\UAS.cdNa} suppresses the overproliferation phenotype seen in scrib¹, Ras85D^V12.Scer\UAS, Scer\GAL4^αTub84B.PL clones.

(Brumby and Richardson, 2003)

Xenogenetic Interactions

Statement

Reference

The Scer\GAL4^en.PU-driven co-expression of BacA\p35^UAS.cHa and dap^UAS.cdNa leads to a longer sustained DNA damage response to ionizing ration (assessed by phospho-H2Av immunostaining) in the wing disc posterior compartment as compared to the BacA\p35^UAS.cHa single expression or wild-type backgrounds.

(Dekanty et al., 2015)

Expression of dap^{Scer\UAS.cdNa} partially rescues the cell death and R7 photoreceptor loss seen when p53^GUS.PB is expressed in third instar larval eye discs under the control of Scer\GAL4^GMR.PU. The adult eye ablation phenotype is also partially rescued.

(Fan et al., 2010)

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{S396A.S404A.Scer\UAS}-induced eye phenotype.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{T231A.S235A.Scer\UAS}-induced eye phenotype.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^{S202A.T205A.Scer\UAS}-induced eye phenotype.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa substantially suppresses the Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa-induced eye phenotype.

(Steinhilb et al., 2007)

The rough-eye phenotype observed in animals expressing Hsap\MAPT^E14.Scer\UAS under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

Co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu significantly reduces the number of dying cells observed. Vacuolar degeneration of the lamina is rescued in these animals.

Co-expression of dap^{Scer\UAS.cdNa} and cdc2^{E51Q.Scer\UAS} in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu significantly reduces the number of dying cells observed.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and cdc2^{E51Q.Scer\UAS}.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Rheb^AV4. This enhancement is blocked by co-expressing dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye under the control of Scer\GAL4^GMR.PF is not modified by co-expression of dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

(Khurana et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

J. de Nooij M.G. Wang I.K. Hariharan

(Tseng and Hariharan, 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

dap^{Scer\UAS.cdNa}

dap^UAS.cdNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Nooij

(Tseng and Hariharan, 2002)

Secondary FlyBase IDs

References (18)

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