FlyBase Allele Report: Dmel\CycA[UAS.cWa]

General Information

Symbol

Dmel\CycA^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Weiss

FlyBase ID

FBal0086884

Feature type

allele

Associated gene

Dmel\CycA

Associated Insertion(s)

Carried in Construct

P{UAS-CycA.W}

Also Known As

UAS-cycA, UAS-cyclin A

Key Links

Transgenic product class

cDNA

(Weiss et al., 1998)

wild_type

(Weiss et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Weiss et al., 1998)

wild_type

(Weiss et al., 1998)

Mutagen

in vitro construct

(Weiss et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-CycA.W}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA fragment that encodes the complete CycA open reading frame.

(Weiss et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CycA

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by dap^UAS.cdNa, Hsap\MAPT^R406W.UAS

(Khurana et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

Phenotype Manifest In

embryonic/larval salivary gland | embryonic stage, with Scer\GAL4^F4

(Weiss et al., 1998)

eye disc, with Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

Detailed Description

Statement

Reference

The adulthood-only expression of CycA^UAS.cWa under the control of Scer\GAL4^C587 and Gal80[ts] does not affect the tissue homeostasis of adult testes, as compared to controls.

(Zhang et al., 2017)

Expression of CycA^Scer\UAS.cWa driven by Scer\GAL4^nos.PG does not significantly affect size and architecture of the testes and germline stem cell maintenance is normal.

(Kotov et al., 2016)

Expression of CycA^Scer\UAS.cWa in the brain under the control of Scer\GAL4^elav.PLu does not result in a neurodegeneration phenotype.

(Khurana et al., 2006)

Expression of CycA^Scer\UAS.cWa under the control of Scer\GAL4^GMR.PF produces excess mitoses in the eye disc.

(Baonza and Freeman, 2005)

Scer\GAL4^F4-mediated expression causes severe inhibition of endoreplication and growth in occasional, apparently randomly distributed cells of the larval salivary glands.

(Weiss et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^GMR.PF has increased occurrence of cell division | larval stage phenotype, non-enhanceable by Delta^UAS.cJa, Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

NOT suppressed by

Statement

Reference

CycA^UAS.cWa, CycE^UAS.cLa, Scer\GAL4^GMR.PF has increased occurrence of cell division | larval stage phenotype, non-suppressible by Delta^UAS.cJa, Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

Enhancer of

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^elav.PLu is an enhancer of increased cell death | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Suppressor of

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^en-e16E is a suppressor | partially of abnormal size | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

CycA^UAS.cWa, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

CycA^UAS.cWa, Scer\GAL4^prd.RG1 is a suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of Scer\GAL4^prd.RG1, fzr^UAS.cSa

(Reber et al., 2006)

CycA^UAS.cWa, Scer\GAL4^prd.RG1 is a suppressor of abnormal mitotic cell cycle | embryonic stage phenotype of Scer\GAL4^prd.RG1, fzr^psm.UAS

(Reber et al., 2006)

NOT Suppressor of

Statement

Reference

E2f1^UAS.cNa, Dp^UAS.cDa, CycA^UAS.cWa, Scer\GAL4^GMR.PF is a non-suppressor of abnormal mitotic cell cycle | somatic clone phenotype of Delta^RevF10

(Baonza and Freeman, 2005)

Other

Statement

Reference

CycA^UAS.cWa, CycE^UAS.cLa, Scer\GAL4^GMR.PF has increased occurrence of cell division | larval stage phenotype

(Baonza and Freeman, 2005)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^GMR.PF has eye disc phenotype, non-enhanceable by Delta^UAS.cJa, Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

NOT suppressed by

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^GMR.PF has eye disc phenotype, non-suppressible by Delta^UAS.cJa, Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

Enhancer of

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^elav.PLu is an enhancer of adult brain | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

CycA^UAS.cWa, Scer\GAL4^elav.PLu is an enhancer of lamina | conditional phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

CycA^UAS.cWa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

CycA^UAS.cWa/Scer\GAL4^GMR.PF is an enhancer of eye | conditional phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PLu

(Khurana et al., 2006)

Suppressor of

Statement

Reference

CycA^UAS.cWa, Scer\GAL4^en-e16E is a suppressor | partially of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^en-e16E, dome^ΔCYT.UAS

(Recasens-Alvarez et al., 2017)

CycA^UAS.cWa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of E2f1^RNAi.UAS.cJa, Scer\GAL4^GMR.PU

(Ji et al., 2012)

CycA^UAS.cWa, Scer\GAL4^prd.RG1 is a suppressor of embryonic epidermis phenotype of Scer\GAL4^prd.RG1, fzr^UAS.cSa

(Reber et al., 2006)

CycA^UAS.cWa, Scer\GAL4^prd.RG1 is a suppressor of embryonic epidermis phenotype of Scer\GAL4^prd.RG1, fzr^psm.UAS

(Reber et al., 2006)

NOT Suppressor of

Statement

Reference

E2f1^UAS.cNa, Dp^UAS.cDa, CycA^UAS.cWa, Scer\GAL4^GMR.PF is a non-suppressor of eye disc | somatic clone phenotype of Delta^RevF10

(Baonza and Freeman, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of CycA^Scer\UAS.cWa partially suppresses the severe decrease in size induced in the third instar wing disc posterior compartment by the expression of dome^{ΔCYT.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Recasens-Alvarez et al., 2017)

The strong rough eye phenotype seen in flies expressing multiple copies of E2f^{dsRNA.Scer\UAS.cJa} under the control of Scer\GAL4^GMR.PU is partially suppressed by co-expression of CycA^Scer\UAS.cWa.

(Ji et al., 2012)

Coexpression of CycA^Scer\UAS.cWa with rap^Scer\UAS.cSa, under the control of Scer\GAL4^prd.RG1, suppresses the inhibition of embryonic mitosis 16 seen when rap^Scer\UAS.cSa is expressed alone. Scer\GAL4^prd.RG1>CycA^Scer\UAS.cWa expression also suppresses the same phenotype in rap^psm.Scer\UAS mutants.

(Reber et al., 2006)

Co-expression of Dl^Scer\UAS.cJa does not significantly modify the excess mitoses phenotype caused by Scer\GAL4^GMR.PF-driven expression of CycA^Scer\UAS.cWa.

Co-overexpression of CycA^Scer\UAS.cWa, E2f^Scer\UAS.cNa and Dp^Scer\UAS.cDa (using Scer\GAL4^GMR.PF) does not significantly rescue the S phase phenotype seen in Dl^RevF10 clones in a Minute/+ background.

(Baonza and Freeman, 2005)

Xenogenetic Interactions

Statement

Reference

Expression of CycA^Scer\UAS.cWa in adult brains expressing Hsap\MAPT^{R406W.Scer\UAS} under the control of Scer\GAL4^elav.PLu significantly increases the number of dying cells observed. Vacuolar degeneration of the lamina is enhanced in these animals.

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of CycA^Scer\UAS.cWa.

(Khurana et al., 2006)

Complementation and Rescue Data

Partially rescues

Scer\GAL4^prd.RG1/CycA^UAS.cWa partially rescues CycA^C8LR1

(Reber et al., 2006)

Comments

Expression of CycA^Scer\UAS.cWa driven by Scer\GAL4^prd.RG1 rescues the mitosis 16 in epidermal cells in CycA^C8LR1 embryos in the segments where CycA^Scer\UAS.cWa is expressed. The intervening segments show the CycA^C8LR1 mitotic phenotype.

(Reber et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6633

w^*; P{UAS-CycA.W}II.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CycA^Scer\UAS.cWa

CycA^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Weiss

(Weiss et al., 1998)

Secondary FlyBase IDs

References (15)

Report Sections

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