FlyBase Allele Report: Dmel\exex[KK30]

General Information

Symbol

Dmel\exex^KK30

Species

D. melanogaster

Name

FlyBase ID

FBal0137268

Feature type

allele

Associated gene

Dmel\exex

Associated Insertion(s)

Carried in Construct

Also Known As

hb9^KK30, dHb9^KK30

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Broihier and Skeath, 2002)

Mutagen

ethyl methanesulfonate

(Broihier and Skeath, 2002)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C646T.

(Broihier and Skeath, 2002)

Amino acid replacement: Q?term.

(Broihier and Skeath, 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:7,949,011..7,949,011 [+]

Nucleotide change:

C7949011T

Reported nucleotide change:

C646T

Amino acid change:

Q216term | exex-PA

Reported amino acid change:

Q?term

(Broihier and Skeath, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Broihier and Skeath, 2002)

abnormal neuroanatomy (with exex^JJ154)

(Miguel-Aliaga et al., 2008)

abnormal neuroanatomy | embryonic stage (with exex^Gal4)

(Oyallon et al., 2012)

Phenotype Manifest In

adult dMP2 Ilp7 neuron (with exex^JJ154)

(Miguel-Aliaga et al., 2008)

embryonic ventral nervous system & neuron

(Broihier and Skeath, 2002)

larval intersegmental nerve

(Broihier and Skeath, 2002)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage (with exex^Gal4)

(Oyallon et al., 2012)

larval transverse nerve | embryonic stage (with exex^Gal4)

(Oyallon et al., 2012)

Detailed Description

Statement

Reference

exex^KK30/exex^Gal4 embryos show motorneuron axon targeting defects. In 34.4% of hemisegments, Fas2-positive ISNb motorneuron axons do not correctly defasciculate from each other, resulting in the stalling of thicker axon bundles between muscles 6 and 13 and a failure to innervate the muscle 12/13 cleft. In 36.2% of hemisegments, ISNb or TN axons extend processes that form abnormal contacts between them.

(Oyallon et al., 2012)

68.3% of exex^KK30/exex^JJ154 mutant anterior dMP2 neurons fail to undergo apoptosis. Other aspects of dMP2 specification appear unaffected; they are able to project neurons posteriorly along the most medial fascicle, and exit the VNC to innervate the hindgut correctly.

(Miguel-Aliaga et al., 2008)

Two ectopic eve-positiver neurons appear per hemisegment, in the vicinity of the aCC/pCC pair, at late stage 11. One remains immediately posterior to aCC/pCC, the other migrates posteriorly. The ectopic neurons arise from the NB1-1 lineage. ISNb projects aberrantly after defasciculating from the ISN, or fails to defasciculate at all.

(Broihier and Skeath, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

exex^Gal4/exex^KK30 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by Oli^Δ9/Oli^Δ9

(Oyallon et al., 2012)

Enhancer of

Statement

Reference

exex^Gal4/exex^KK30 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Oli^Δ9

(Oyallon et al., 2012)

NOT Enhancer of

Statement

Reference

exex[+]/exex^KK30 is a non-enhancer of abnormal neuroanatomy | dominant phenotype of vvl^dfr-B129

(Certel and Thor, 2004)

NOT Suppressor of

Statement

Reference

exex[+]/exex^KK30 is a non-suppressor | somatic clone of abnormal neuroanatomy | somatic clone | adult stage phenotype of pros^IG2227

(Hartl et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

exex^Gal4/exex^KK30 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, enhanceable by Oli^Δ9/Oli^Δ9

(Oyallon et al., 2012)

Enhancer of

Statement

Reference

exex^Gal4/exex^KK30 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Oli^Δ9

(Oyallon et al., 2012)

NOT Enhancer of

Statement

Reference

exex^KK30 is a non-enhancer of larval intersegmental nerve | embryonic stage 16 phenotype of HGTX^D25

(Broihier et al., 2004)

exex[+]/exex^KK30 is a non-enhancer of larval intersegmental nerve | embryonic stage 16 phenotype of vvl^dfr-B129

(Certel and Thor, 2004)

exex[+]/exex^KK30 is a non-enhancer of larval transverse nerve | embryonic stage 16 phenotype of vvl^dfr-B129

(Certel and Thor, 2004)

NOT Suppressor of

Statement

Reference

exex[+]/exex^KK30 is a non-suppressor | somatic clone of carbon dioxide sensitive neuron | ectopic | somatic clone phenotype of pros^IG2227

(Hartl et al., 2011)

Other

Statement

Reference

HGTX^D25, exex^KK30 has embryonic central nervous system & neuron | ectopic phenotype

(Broihier et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the phenotype seen in both Oli^Δ9 (19.3%) and exex^KK30/exex^Gal4 (34.4%) single mutant embryos where ISNb motorneuron axons do not correctly defasciculate from each other and fail to innervate the muscle 12/13 cleft is increased in double mutants to 68.5%.

(Oyallon et al., 2012)

The number of ectopic CO[[2]] neurons on the maxillary palp in animals containing pros^IG2227 clones is unaffected if they are also carrying exex^KK30/+.

(Hartl et al., 2011)

Embryos doubly mutant for H15^nmr-210 or mid^GA174 and exex^KK30 do not show enhancement of the respective single mutant central nervous system phenotypes.

(Leal et al., 2009)

Stage 15 exex^KK30; HGTX^D25 double mutant embryos have ectopic vnd expressing and eve expressing neurons in the central nervous system.

(Broihier et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

exex^JJ154/exex^KK30 is rescued by exex^UAS.cOa/Scer\GAL4^odd-106

(Miguel-Aliaga et al., 2008)

Not rescued by

exex^JJ154/exex^KK30 is not rescued by exex^UAS.cOa/Scer\GAL4^Vap.P0201

(Miguel-Aliaga et al., 2008)

Comments

Expression of exex^Scer\UAS.cOa from embryonic stage 16 onwards under the control of Scer\GAL4^Vap.P0201 fails to rescue the apoptosis defects seen in exex^KK30/exex^JJ154 mutant anterior dMP2 neurons. Expressing exex^Scer\UAS.cOa from stage 10 under the control of Scer\GAL4^odd-106 does rescue this apoptosis.

(Miguel-Aliaga et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9930

w^*; exex^KK30 e^s/TM3, P{ftz-lacZ.ry⁺}TM3, Sb¹ ry^*

56828

Df(3L)D25, exex^KK30 HGTX^D25/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Allele is a protein null.

(Broihier and Skeath, 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Hb9^KK30

(Garces and Thor, 2006)

dHb9^KK30

(Broihier and Skeath, 2002)

dhb9^kk30

(Leal et al., 2009)

exex^KK30

hb9^KK30

(Oyallon et al., 2012, Miguel-Aliaga et al., 2008, Broihier et al., 2004, Certel and Thor, 2004)

hb9^kk30

(Santiago and Bashaw, 2017, Lacin et al., 2014, Santiago et al., 2014, Hartl et al., 2011, Lacin et al., 2009, Leal et al., 2009, Layden et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (13)

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