FlyBase Allele Report: Dmel\HGTX[D25]

General Information

Symbol

Dmel\HGTX^D25

Species

D. melanogaster

Name

FlyBase ID

FBal0183335

Feature type

allele

Associated gene

Dmel\HGTX

Associated Insertion(s)

Carried in Construct

Also Known As

nkx6^D25

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Broihier et al., 2004)

Progenitor genotype

HGTX^P-JG

(Broihier et al., 2004)

Caused by aberration

Df(3L)D25

Cytology

Description

A 25kb deletion, generated by imprecise excision of P{lacZ}HGTX^P-JG, that removes the 3' end of HGTX and the complete ORF of CG13479.

(Broihier et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Broihier et al., 2004)

abnormal neuroanatomy | third instar larval stage

(Lacin et al., 2014)

Phenotype Manifest In

interneuron & axon & longitudinal connective

(Broihier et al., 2004)

larval intersegmental nerve | embryonic stage 16

(Broihier et al., 2004)

larval intersegmental nerve | embryonic stage 16 (with Df(3L)fz-D21)

(Broihier et al., 2004)

larval intersegmental nerve | embryonic stage 16 (with HGTX^P-JG)

(Broihier et al., 2004)

RP neuron & axon & embryo

(Broihier et al., 2004)

Detailed Description

Statement

Reference

A lineage 3 HGTX^D25 mutant postembryonic neuroblast clone examined at the third larval instar stage contains the 3id axon bundle, but lacks the 3il bundle normally produced by this lineage.

(Lacin et al., 2014)

In stage 16 HGTX^D25 homozygous embryos, both secondary branches of the intersegmental nerve (anterior fascicle), ISNb and ISNd, are absent in a significant proportion of hemisegments. This phenotype is also seen in HGTX^D25/HGTX^P-JG and HGTX^D25/Df(3L)fz-D21 embryos. exex expressing interneurons largely fail to project axons along the longitudinal fascicles in stage 16 HGTX^D25 homozygous embryos. In HGTX^D25 homozygous embryos, RP motor axons only exit the CNS 39% of hemisegments compared to 86% in wild-type. These axons often appear thinner than wild-type and the morphology of truncated axons is often aberrant - growth cones are frequently enlarged and have a club-like appearance. In 10% of mutant hemisegments, RP motor axons make dramatic guidance errors, often turning back inappropriately and extending toward the midline.

(Broihier et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

HGTX^D25 is a non-enhancer of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav-C155, exex^UAS.cBa

(Lacin et al., 2014)

NOT Suppressor of

Statement

Reference

HGTX^D25 is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav-C155, exex^UAS.cBa

(Lacin et al., 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

HGTX^D25 has larval intersegmental nerve | embryonic stage 16 phenotype, non-enhanceable by exex^KK30

(Broihier et al., 2004)

Enhancer of

Statement

Reference

HGTX^D25 is an enhancer of presumptive embryonic/larval central nervous system phenotype of mid^GA174

(Leal et al., 2009)

NOT Enhancer of

Statement

Reference

HGTX^D25 is a non-enhancer of neuron | embryonic stage | lateral phenotype of Scer\GAL4^elav-C155, exex^UAS.cBa

(Lacin et al., 2014)

NOT Suppressor of

Statement

Reference

HGTX^D25 is a non-suppressor of neuron | lateral | embryonic stage phenotype of Scer\GAL4^elav-C155, exex^UAS.cBa

(Lacin et al., 2014)

Other

Statement

Reference

HGTX^D25, exex^KK30 has embryonic central nervous system & neuron | ectopic phenotype

(Broihier et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

A homozygous HGTX^D25 mutant background does not alter the ectopic lateral fd59A[+] neurons seen when exex^Scer\UAS.cBa is expressed under the control of Scer\GAL4^elav-C155.

(Lacin et al., 2014)

Embryos doubly mutant for mid^GA174 and HGTX^D25 exhibit gross defects in the architecture of the central nervous system.

(Leal et al., 2009)

Stage 15 exex^KK30; HGTX^D25 double mutant embryos have ectopic vnd expressing neurons in the central nervous system. exex^KK30/exex^KK30 does not enhance the expressivity of loss of secondary branches of the intersegmental nerve (anterior fascicle) - ISNb and ISNd - in stage 16 HGTX^D25 homozygous embryos.

(Broihier et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

HGTX^GAL4/HGTX^D25 is partially rescued by Scer\GAL4^HGTX-GAL4/HGTX^UAS.cBa

(Broihier et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

56828

Df(3L)D25, exex^KK30 HGTX^D25/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

HGTX^D25

Nkx6^D25

(Seibert and Urbach, 2010, Seibert et al., 2009)

Nkx6^d25

(Lacin et al., 2009)

nkx6^D25

(Lacin et al., 2014, Santiago et al., 2014, Leal et al., 2009)

nkx6^D26

(Leal et al., 2009)

nkx6^d25

(Lacin et al., 2014)

nkx6^Δ25

(Layden et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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